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Team:XMU-China/protocolsindex
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| - | <table width="900" border="3"> | + | <table width="900" border="3" id="contents"> |
<tr> | <tr> | ||
| - | <th>Contents<span id="hide"><a href="#" onClick="MM_changeProp('indextd4','','display','none','TD');MM_changeProp('indextd1','','display','none','TD');MM_changeProp('indextd2','','display','none','TD');MM_changeProp('indextd3','','display','none','TD');MM_changeProp('hide','','display','none','SPAN');MM_changeProp('indextd5','','display','none','TD');MM_changeProp('indextd6','','display','none','TD');MM_changeProp('indextd7','','display','none','TD');MM_changeProp('indextd8','','display','none','TD');MM_changeProp('indextd9','','display','none','TD');MM_changeProp('indextd10','','display','none','TD');MM_changeProp('indextd11','','display','none','TD');MM_changeProp('indextd12','','display','none','TD');MM_changeProp('indextd13','','display','none','TD');MM_changeProp('indextd14','','display','none','TD');MM_changeProp('indextd15','','display','none','TD');MM_changeProp('show','','display','block','SPAN');MM_changeProp('indextd16','','display','none','TD')">[hide]</a></span> | + | <th>Contents<span id="hide"><a href="#" onClick="MM_changeProp('indextd4','','display','none','TD');MM_changeProp('indextd1','','display','none','TD');MM_changeProp('indextd2','','display','none','TD');MM_changeProp('indextd3','','display','none','TD');MM_changeProp('hide','','display','none','SPAN');MM_changeProp('indextd5','','display','none','TD');MM_changeProp('indextd6','','display','none','TD');MM_changeProp('indextd7','','display','none','TD');MM_changeProp('indextd8','','display','none','TD');MM_changeProp('indextd9','','display','none','TD');MM_changeProp('indextd10','','display','none','TD');MM_changeProp('indextd11','','display','none','TD');MM_changeProp('indextd12','','display','none','TD');MM_changeProp('indextd13','','display','none','TD');MM_changeProp('indextd14','','display','none','TD');MM_changeProp('indextd15','','display','none','TD');MM_changeProp('show','','display','block','SPAN');MM_changeProp('indextd16','','display','none','TD');MM_changeProp('indextd17','','display','none','TD');MM_changeProp('indextd18','','display','none','TD');MM_changeProp('indextd19','','display','none','TD');MM_changeProp('indextd20','','display','none','TD');MM_changeProp('indextd21','','display','none','TD');MM_changeProp('indextd22','','display','none','TD');MM_changeProp('indextd23','','display','none','TD');MM_changeProp('indextd24','','display','none','TD');MM_changeProp('indextd25','','display','none','TD')">[hide]</a></span> |
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| - | <td id="indextd1"><li id="indexli"><a id="indexa" href="#_Toc01"><strong>1 | + | <td id="indextd1"><li id="indexli"><a id="indexa" href="#_Toc01"><strong>1 General protocols</strong></a></li> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td id="indextd2"><li id="indexli"><a id="indexa" href="#_Toc02"><strong> | + | <td id="indextd2"><li id="indexli"><a id="indexa" href="#_Toc02"><strong> 1.1 Stock solution</strong></a></li></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td id="indextd3"><li id="indexli"><a id="indexa" href="#_Toc03"><strong> | + | <td id="indextd3"><li id="indexli"><a id="indexa" href="#_Toc03"><strong> 1.2 Preparation of Competent BL21</strong></a><a href="#_Toc03"> </a></li></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td id="indextd4"><li id="indexli"><a id="indexa" href="#_Toc04"><strong> | + | <td id="indextd4"><li id="indexli"><a id="indexa" href="#_Toc04"><strong> 1.3 Transformation</strong></a></li> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td id="indextd5"><li id="indexli"><a id="indexa" href="#_Toc05"><strong> | + | <td id="indextd5"><li id="indexli"><a id="indexa" href="#_Toc05"><strong> 1.4 Plasmid Purification</strong></a></li> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td id="indextd6"><li id="indexli"><a id="indexa" href="#_Toc06"><strong> | + | <td id="indextd6"><li id="indexli"><a id="indexa" href="#_Toc06"><strong> 1.5 Reaction system of restriction endonuclease</strong></a></li> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td id="indextd7"><li id="indexli"><a id="indexa" href="#_Toc07"><strong> | + | <td id="indextd7"><li id="indexli"><a id="indexa" href="#_Toc07"><strong> 1.6 Standard BioBrick Assembly</strong></a></li> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td id="indextd8"><li id="indexli"><a id="indexa" href="#_Toc08"><strong> | + | <td id="indextd8"><li id="indexli"><a id="indexa" href="#_Toc08"><strong> 1.7 Suffix Insertion</strong></a></li> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td id="indextd9"><li id="indexli"><a id="indexa" href="#_Toc09"><strong> | + | <td id="indextd9"><li id="indexli"><a id="indexa" href="#_Toc09"><strong> 1.8 Ligation</strong></a></li> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td id="indextd10"><li id="indexli"><a id="indexa" href="#_Toc10"><strong> | + | <td id="indextd10"><li id="indexli"><a id="indexa" href="#_Toc10"><strong> 1.9 Restriction analysis</strong></a></li> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td id="indextd11"><li id="indexli"><a id="indexa" href="#_Toc11"><strong> | + | <td id="indextd11"><li id="indexli"><a id="indexa" href="#_Toc11"><strong> 1.10 Gel Extraction</strong></a></li> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td id="indextd12"><li id="indexli"><a id="indexa" href="#_Toc12"><strong>2 | + | <td id="indextd12"><li id="indexli"><a id="indexa" href="#_Toc12"><strong>2 Characterization</strong></a></li> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td id="indextd13"><li id="indexli"><a id="indexa" href="#_Toc13"><strong> | + | <td id="indextd13"><li id="indexli"><a id="indexa" href="#_Toc13"><strong> 2.1 Fluorescence Measurements </strong></a></li> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td id="indextd14"><li id="indexli"><a id="indexa" href="#_Toc14"><strong> | + | <td id="indextd14"><li id="indexli"><a id="indexa" href="#_Toc14"><strong> 2.2 Protein electrophoresis</strong></a></li> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td id="indextd15"><li id="indexli"><a id="indexa" href="#_Toc15"><strong> | + | <td id="indextd15"><li id="indexli"><a id="indexa" href="#_Toc15"><strong> 2.2.1 Preparation of Samples</strong></a></li> </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc16"><strong> | + | <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc16"><strong> 2.2.2 Manufacture Albumen Gel</strong></a></li> </td> |
</tr> | </tr> | ||
| + | <tr> | ||
| + | <td id="indextd17"><li id="indexli"><a id="indexa" href="#_Toc17"><strong> 2.2.3 Electrophoresis</strong></a></li> </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td id="indextd18"><li id="indexli"><a id="indexa" href="#_Toc18"><strong> 2.2.4 Dyeing (Colloidal Coomassie Brilliant Blue)</strong></a></li> </td> | ||
| + | </tr> | ||
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| + | <td id="indextd19"><li id="indexli"><a id="indexa" href="#_Toc19"><strong> 2.2.5 Scanning</strong></a></li> </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td id="indextd20"><li id="indexli"><a id="indexa" href="#_Toc20"><strong>3 Immobilization</strong></a></li> </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td id="indextd21"><li id="indexli"><a id="indexa" href="#_Toc21"><strong> 3.1 Prepare Sodiumcellu-losesulfate (NaCS)</strong></a></li> </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td id="indextd22"><li id="indexli"><a id="indexa" href="#_Toc22"><strong> 3.2 Prepare microcapsules</strong></a></li> </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td id="indextd23"><li id="indexli"><a id="indexa" href="#_Toc23"><strong> 3.3 Immobilize cells into calcium alginate beads</strong></a></li> </td> | ||
| + | </tr> | ||
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| + | <tr> | ||
| + | <td id="indextd24"><li id="indexli"><a id="indexa" href="#_Toc24"><strong> 3.4 Immobilize cells into intra-hollow Ca-alginate capsules</strong></a></li> </td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td id="indextd25"><li id="indexli"><a id="indexa" href="#_Toc25"><strong> 3.5 Immobilize cells into NaCS-PDMDAAC microcapsules</strong></a></li> </td> | ||
| + | </tr> | ||
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</table> | </table> | ||
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Latest revision as of 19:36, 26 September 2012
