Team:XMU-China/protocolsindex

From 2012.igem.org

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       <th>Contents<span id="hide"><a href="#" onClick="MM_changeProp('indextd4','','display','none','TD');MM_changeProp('indextd1','','display','none','TD');MM_changeProp('indextd2','','display','none','TD');MM_changeProp('indextd3','','display','none','TD');MM_changeProp('hide','','display','none','SPAN');MM_changeProp('indextd5','','display','none','TD');MM_changeProp('indextd6','','display','none','TD');MM_changeProp('indextd7','','display','none','TD');MM_changeProp('indextd8','','display','none','TD');MM_changeProp('indextd9','','display','none','TD');MM_changeProp('indextd10','','display','none','TD');MM_changeProp('indextd11','','display','none','TD');MM_changeProp('indextd12','','display','none','TD');MM_changeProp('indextd13','','display','none','TD');MM_changeProp('indextd14','','display','none','TD');MM_changeProp('indextd15','','display','none','TD');MM_changeProp('show','','display','block','SPAN');MM_changeProp('indextd16','','display','none','TD')">[hide]</a></span>
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       <th>Contents<span id="hide"><a href="#" onClick="MM_changeProp('indextd4','','display','none','TD');MM_changeProp('indextd1','','display','none','TD');MM_changeProp('indextd2','','display','none','TD');MM_changeProp('indextd3','','display','none','TD');MM_changeProp('hide','','display','none','SPAN');MM_changeProp('indextd5','','display','none','TD');MM_changeProp('indextd6','','display','none','TD');MM_changeProp('indextd7','','display','none','TD');MM_changeProp('indextd8','','display','none','TD');MM_changeProp('indextd9','','display','none','TD');MM_changeProp('indextd10','','display','none','TD');MM_changeProp('indextd11','','display','none','TD');MM_changeProp('indextd12','','display','none','TD');MM_changeProp('indextd13','','display','none','TD');MM_changeProp('indextd14','','display','none','TD');MM_changeProp('indextd15','','display','none','TD');MM_changeProp('show','','display','block','SPAN');MM_changeProp('indextd16','','display','none','TD');MM_changeProp('indextd17','','display','none','TD');MM_changeProp('indextd18','','display','none','TD');MM_changeProp('indextd19','','display','none','TD');MM_changeProp('indextd20','','display','none','TD');MM_changeProp('indextd21','','display','none','TD');MM_changeProp('indextd22','','display','none','TD');MM_changeProp('indextd23','','display','none','TD');MM_changeProp('indextd24','','display','none','TD');MM_changeProp('indextd25','','display','none','TD')">[hide]</a></span>
          
          
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   <span id="show"><a href="#" onClick="MM_changeProp('hide','','display','block','SPAN');MM_changeProp('show','','display','none','SPAN');MM_changeProp('indextd1','','display','block','TD');MM_changeProp('indextd2','','display','block','TD');MM_changeProp('indextd3','','display','block','TD');MM_changeProp('indextd4','','display','block','TD');MM_changeProp('indextd5','','display','block','TD');MM_changeProp('indextd6','','display','block','TD');MM_changeProp('indextd7','','display','block','TD');MM_changeProp('indextd8','','display','block','TD');MM_changeProp('indextd9','','display','block','TD');MM_changeProp('indextd10','','display','block','TD');MM_changeProp('indextd11','','display','block','TD');MM_changeProp('indextd12','','display','block','TD');MM_changeProp('indextd13','','display','block','TD');MM_changeProp('indextd14','','display','block','TD');MM_changeProp('indextd15','','display','block','TD');MM_changeProp('indextd16','','display','block','TD')">[show]</a></span></th>
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   <span id="show"><a href="#" onClick="MM_changeProp('hide','','display','block','SPAN');MM_changeProp('show','','display','none','SPAN');MM_changeProp('indextd1','','display','block','TD');MM_changeProp('indextd2','','display','block','TD');MM_changeProp('indextd3','','display','block','TD');MM_changeProp('indextd4','','display','block','TD');MM_changeProp('indextd5','','display','block','TD');MM_changeProp('indextd6','','display','block','TD');MM_changeProp('indextd7','','display','block','TD');MM_changeProp('indextd8','','display','block','TD');MM_changeProp('indextd9','','display','block','TD');MM_changeProp('indextd10','','display','block','TD');MM_changeProp('indextd11','','display','block','TD');MM_changeProp('indextd12','','display','block','TD');MM_changeProp('indextd13','','display','block','TD');MM_changeProp('indextd14','','display','block','TD');MM_changeProp('indextd15','','display','block','TD');MM_changeProp('indextd16','','display','block','TD');MM_changeProp('indextd17','','display','block','TD');MM_changeProp('indextd18','','display','block','TD');MM_changeProp('indextd19','','display','block','TD');MM_changeProp('indextd20','','display','block','TD');MM_changeProp('indextd21','','display','block','TD');MM_changeProp('indextd22','','display','block','TD');MM_changeProp('indextd23','','display','block','TD');MM_changeProp('indextd24','','display','block','TD');MM_changeProp('indextd25','','display','block','TD')">[show]</a></span></th>
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       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc17"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.3&nbsp;Electrophoresis</strong></a></li> </td>
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       <td id="indextd17"><li id="indexli"><a id="indexa" href="#_Toc17"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.3&nbsp;Electrophoresis</strong></a></li> </td>
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       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc18"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.4&nbsp;Dyeing (Colloidal Coomassie Brilliant Blue)</strong></a></li> </td>
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       <td id="indextd18"><li id="indexli"><a id="indexa" href="#_Toc18"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.4&nbsp;Dyeing (Colloidal Coomassie Brilliant Blue)</strong></a></li> </td>
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       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc19"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.5&nbsp;Scanning</strong></a></li> </td>
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       <td id="indextd19"><li id="indexli"><a id="indexa" href="#_Toc19"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;2.2.5&nbsp;Scanning</strong></a></li> </td>
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       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc20"><strong>3 Immobilization</strong></a></li> </td>
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       <td id="indextd20"><li id="indexli"><a id="indexa" href="#_Toc20"><strong>3 Immobilization</strong></a></li> </td>
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       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc21"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.1&nbsp;Prepare Sodiumcellu-losesulfate (NaCS)</strong></a></li> </td>
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       <td id="indextd21"><li id="indexli"><a id="indexa" href="#_Toc21"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.1&nbsp;Prepare Sodiumcellu-losesulfate (NaCS)</strong></a></li> </td>
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       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc22"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.2&nbsp;Prepare microcapsules</strong></a></li> </td>
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       <td id="indextd22"><li id="indexli"><a id="indexa" href="#_Toc22"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.2&nbsp;Prepare microcapsules</strong></a></li> </td>
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       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc23"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.3&nbsp;Immobilize cells into calcium alginate beads</strong></a></li> </td>
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       <td id="indextd23"><li id="indexli"><a id="indexa" href="#_Toc23"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.3&nbsp;Immobilize cells into calcium alginate beads</strong></a></li> </td>
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       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc24"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.4&nbsp;Immobilize cells into intra-hollow Ca-alginate capsules</strong></a></li> </td>
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       <td id="indextd24"><li id="indexli"><a id="indexa" href="#_Toc24"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.4&nbsp;Immobilize cells into intra-hollow Ca-alginate capsules</strong></a></li> </td>
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       <td id="indextd16"><li id="indexli"><a id="indexa" href="#_Toc25"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.5&nbsp;Immobilize cells into NaCS-PDMDAAC microcapsules</strong></a></li> </td>
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       <td id="indextd25"><li id="indexli"><a id="indexa" href="#_Toc25"><strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;3.5&nbsp;Immobilize cells into NaCS-PDMDAAC microcapsules</strong></a></li> </td>
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Revision as of 14:43, 23 September 2012

safetyindex

Contents[hide] [show]
  • 1 General protocols
  •        1.1 Stock solution
  •        1.2 Preparation of Competent BL21
  •        1.3 Transformation
  •        1.4 Plasmid Purification
  •        1.5 Reaction system of restriction endonuclease
  •        1.6 Standard BioBrick Assembly
  •        1.7 Suffix Insertion
  •        1.8 Ligation
  •        1.9 Restriction analysis
  •        1.10 Gel Extraction
  • 2 Characterization
  •        2.1 Fluorescence Measurements
  •        2.2 Protein electrophoresis
  •               2.2.1 Preparation of Samples
  •               2.2.2 Manufacture Albumen Gel
  •               2.2.3 Electrophoresis
  •               2.2.4 Dyeing (Colloidal Coomassie Brilliant Blue)
  •               2.2.5 Scanning
  • 3 Immobilization
  •        3.1 Prepare Sodiumcellu-losesulfate (NaCS)
  •        3.2 Prepare microcapsules
  •        3.3 Immobilize cells into calcium alginate beads
  •        3.4 Immobilize cells into intra-hollow Ca-alginate capsules
  •        3.5 Immobilize cells into NaCS-PDMDAAC microcapsules