Team:Warsaw/Safety
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not hazardous. Bacillus strain carrying a plasmid with listeriolysin | not hazardous. Bacillus strain carrying a plasmid with listeriolysin | ||
might be hazardous, so great care must be taken. But even if one does | might be hazardous, so great care must be taken. But even if one does | ||
- | become infected, | + | become infected, these bacteria are unable of replicating and surviving |
long inside mammalian cells. They are also incapable of spreading from | long inside mammalian cells. They are also incapable of spreading from | ||
cell to cell, meaning that infection could not spread. | cell to cell, meaning that infection could not spread. | ||
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None of our project's ideas can cause public safety hazard. We are not | None of our project's ideas can cause public safety hazard. We are not | ||
going to transform our multihost eucaryotic vector into invasive | going to transform our multihost eucaryotic vector into invasive | ||
- | Bacillus strain, to ensure | + | Bacillus strain, to ensure that no danger is caused. Listeriolysin |
might raise some issues, that is why we adopted rigorous safety | might raise some issues, that is why we adopted rigorous safety | ||
protocols concerning disposal of biological waste, to prevent any GMO | protocols concerning disposal of biological waste, to prevent any GMO | ||
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None of the Biobricks that we use this year may cause environmental | None of the Biobricks that we use this year may cause environmental | ||
hazard. Plasmids containing genes coding resistance for antibiotics | hazard. Plasmids containing genes coding resistance for antibiotics | ||
- | such as | + | such as ampicilin, chloramfenicol and kanamicin might be hazardous if |
- | released into environment, enlarging population of | + | released into environment, enlarging population of antibiotics resistant |
bacteria. To ensure that this does not happen we adopted safety | bacteria. To ensure that this does not happen we adopted safety | ||
protocols, as mentioned above. | protocols, as mentioned above. | ||
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<u>2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?<br/></u> | <u>2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?<br/></u> | ||
- | In our invasion device we use previously created listeriolysin BioBrick(BBa_...), which could be potentially harmful if someone was careless enough to get infected by a bacteria strain carrying this device. Even in this case the danger is limited as strains we use are unable to spread from cell to cell and it is impossible for them to last long in mammalian cells as it was mentioned before. We minimalized the risk by thorough application of our safety protocols.<br /><br /> | + | In our invasion device we use previously created listeriolysin BioBrick (BBa_...), which could be potentially harmful if someone was careless enough to get infected by a bacteria strain carrying this device. Even in this case the danger is limited as strains we use are unable to spread from cell to cell and it is impossible for them to last long in mammalian cells as it was mentioned before. We minimalized the risk by thorough application of our safety protocols.<br /><br /> |
<u>3. Is there a local biosafety group, committee, or review board at your institution?<br /></u> | <u>3. Is there a local biosafety group, committee, or review board at your institution?<br /></u> |
Revision as of 11:04, 7 September 2012
1. Would any of your project ideas raise safety issues in terms of: researcher safety, public safety, or environmental safety? Laboratory strains of E. coli and B. subtilis are not pathogenic, thus
not hazardous. Bacillus strain carrying a plasmid with listeriolysin
might be hazardous, so great care must be taken. But even if one does
become infected, these bacteria are unable of replicating and surviving
long inside mammalian cells. They are also incapable of spreading from
cell to cell, meaning that infection could not spread.
2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? 3. Is there a local biosafety group, committee, or review board at your institution?
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