"
Team:Valencia/protocols
From 2012.igem.org
(Difference between revisions)
| Line 18: | Line 18: | ||
<ul style="list-style-type: square"> | <ul style="list-style-type: square"> | ||
<li>LB Agar Plates</li> | <li>LB Agar Plates</li> | ||
| - | <li>BG-11 | + | <li>BG-11 for Wildtype Liquid</li> |
| - | <li>BG-11 | + | <li>BG-11 for Wildtype Solid</li> |
| - | <li>BG-11 | + | <li>BG-11 for Harvard/Golden Liquid</li> |
| - | <li>BG-11 | + | <li>BG-11 for Harvard/Golden Solid</li> |
| - | <li> | + | <li>Compo Plus Agar</li> |
</ul> | </ul> | ||
<br><br> | <br><br> | ||
Revision as of 01:22, 27 September 2012
Protocols
Culture medium
- LB Agar Plates
- BG-11 for Wildtype Liquid
- BG-11 for Wildtype Solid
- BG-11 for Harvard/Golden Liquid
- BG-11 for Harvard/Golden Solid
- Compo Plus Agar
Culture
- Culturing E.Coli
- Culturing S. elongatus in liquid medium
- Culturing S. elongatus in solid medium
Molecular Tools
- Transforming Chemically Competent Cells E.Coli DH5α-T1R
- PLASMID MINIPREP
- DNA Digestion
- Ligations
- Agarose Gel Electrophoresis
- Transforming: S. elongatus PCC7942
