Team:Valencia/protocols

From 2012.igem.org

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<h4>Culture medium</h4>
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<li>LB Agar Plates</li>
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<li>BG-11 FOR WILDTYPE Liquid<</li>
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<li>BG-11 FOR WILDTYPE Solid</li>
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<li>BG-11 FOR HARVARD/GOLDEN Liquid</li>
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<li>BG-11 FOR HARVARD/GOLDEN Solid</li>
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<li>COMPO PLUS AGAR</li>
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<h4>Culture</h4>
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<li>Culturing <i>E.Coli</i></li>
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<li>Culturing <i>S. elongatus</i> in liquid medium</li>
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<li>Culturing <i>S. elongatus</i> in solid medium</li>
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<h4>Molecular Tools</h4>
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<li>Transforming Chemically Competent Cells <i>E.Coli</i> DH5α-T1<sup>R</sup></li>
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<li>PLASMID MINIPREP</li>
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<li>DNA Digestion</li>
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<li>Ligations</li>
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<li>Agarose Gel Electrophoresis</li>
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<li>Transforming: <i>S. elongatus</i> PCC7942</li>
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Revision as of 01:20, 27 September 2012



Protocols


Culture medium

  • LB Agar Plates
  • BG-11 FOR WILDTYPE Liquid<
  • BG-11 FOR WILDTYPE Solid
  • BG-11 FOR HARVARD/GOLDEN Liquid
  • BG-11 FOR HARVARD/GOLDEN Solid
  • COMPO PLUS AGAR


Culture

  • Culturing E.Coli
  • Culturing S. elongatus in liquid medium
  • Culturing S. elongatus in solid medium


Molecular Tools

  • Transforming Chemically Competent Cells E.Coli DH5α-T1R
  • PLASMID MINIPREP
  • DNA Digestion
  • Ligations
  • Agarose Gel Electrophoresis
  • Transforming: S. elongatus PCC7942

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