http://2012.igem.org/wiki/index.php?title=Team:Valencia/Engineering&feed=atom&action=historyTeam:Valencia/Engineering - Revision history2024-03-29T05:31:33ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Valencia/Engineering&diff=237398&oldid=prevGonzalo at 03:42, 27 September 20122012-09-27T03:42:08Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:42, 27 September 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 53:</td>
<td colspan="2" class="diff-lineno">Line 53:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Air Pump</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Air Pump</h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><del class="diffchange diffchange-inline"><br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This <i>Synechococcus</i> bioreactor was built in a 2l Kitasatos flask with all openings thoroughly sealed with parafilm. Through this controlled interfaces we bored in sterile input and output tubing. The input tube opening falls below the liquid level and is in charge of bubbling air pumped from the environment to remove CO<sub>2</sub> used in photosynthesis. It differs from the previous bioreactor in this aspect, as it focuses on removing environmental CO<sub>2</sub> instead of boosting the culture with high carbonation.<br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>This <i>Synechococcus</i> bioreactor was built in a 2l Kitasatos flask with all openings thoroughly sealed with parafilm. Through this controlled interfaces we bored in sterile input and output tubing. The input tube opening falls below the liquid level and is in charge of bubbling air pumped from the environment to remove CO<sub>2</sub> used in photosynthesis. It differs from the previous bioreactor in this aspect, as it focuses on removing environmental CO<sub>2</sub> instead of boosting the culture with high carbonation.<br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To avoid the contamination problems that happened at the previous design, we attached a clarification filter of 0.45micron pore size to retain the microorganisms from the incoming air flow.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To avoid the contamination problems that happened at the previous design, we attached a clarification filter of 0.45micron pore size to retain the microorganisms from the incoming air flow.<br><br></div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 75:</td>
<td colspan="2" class="diff-lineno">Line 74:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Design:</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>Design:</h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><br></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Set 2 separate culture modules, a flat wide one for <i>S. elongatus cscB</i> as a solar module and a smaller compact one for <i>A. fischeri</i> as a biobulb. Prepare an open system so that there can be gas exchange from the cultures with the atmosphere (to let the system at as a CO<sub>2</sub> sink), but with a Pasteurian design opening to avoid contamination from deposition.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Set 2 separate culture modules, a flat wide one for <i>S. elongatus cscB</i> as a solar module and a smaller compact one for <i>A. fischeri</i> as a biobulb. Prepare an open system so that there can be gas exchange from the cultures with the atmosphere (to let the system at as a CO<sub>2</sub> sink), but with a Pasteurian design opening to avoid contamination from deposition.</div></td></tr>
</table>Gonzalohttp://2012.igem.org/wiki/index.php?title=Team:Valencia/Engineering&diff=237337&oldid=prevGonzalo at 03:41, 27 September 20122012-09-27T03:41:12Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:41, 27 September 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 13:</td>
<td colspan="2" class="diff-lineno">Line 13:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2><b>Simple Batch Bioreactors</b></h2><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2><b>Simple Batch Bioreactors</b></h2><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>CO<sub>2</sub> Pump</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>CO<sub>2</sub> Pump</h3></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><br></del><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To enhance a faster growth rate in our Synechococcus broth culture and taking advantage of the bacterium being photosynthetic, we decided to make a device with a CO2 pump in order to make the transformations quickly. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To enhance a faster growth rate in our Synechococcus broth culture and taking advantage of the bacterium being photosynthetic, we decided to make a device with a CO2 pump in order to make the transformations quickly. </div></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 74:</td>
<td colspan="2" class="diff-lineno">Line 74:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our furthest goal is to build a continuous culture system with spatial and temporal decoupling from the photosynthetic and the bioluminescent module, totally autonomous. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Our furthest goal is to build a continuous culture system with spatial and temporal decoupling from the photosynthetic and the bioluminescent module, totally autonomous. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">b><u</del>>Design:</<del class="diffchange diffchange-inline">u></b</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">h3</ins>>Design:</<ins class="diffchange diffchange-inline">h3</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
</table>Gonzalohttp://2012.igem.org/wiki/index.php?title=Team:Valencia/Engineering&diff=237245&oldid=prevGonzalo at 03:39, 27 September 20122012-09-27T03:39:44Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:39, 27 September 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 12:</td>
<td colspan="2" class="diff-lineno">Line 12:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2><b>Simple Batch Bioreactors</b></h2><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2><b>Simple Batch Bioreactors</b></h2><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">b</del>>CO<sub>2</sub> Pump</<del class="diffchange diffchange-inline">b</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">h3</ins>>CO<sub>2</sub> Pump</<ins class="diffchange diffchange-inline">h3</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 65:</td>
<td colspan="2" class="diff-lineno">Line 65:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">b</del>>Simple biphasic coculture bioreactor:</<del class="diffchange diffchange-inline">b</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">h3</ins>>Simple biphasic coculture bioreactor:</<ins class="diffchange diffchange-inline">h3</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><br><img align="center" src="https://static.igem.org/mediawiki/2012/2/20/IMG_0399.JPG" width="220" higth="170" border="10"></a></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><br><img align="center" src="https://static.igem.org/mediawiki/2012/2/20/IMG_0399.JPG" width="220" higth="170" border="10"></a></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
</table>Gonzalohttp://2012.igem.org/wiki/index.php?title=Team:Valencia/Engineering&diff=237024&oldid=prevGonzalo at 03:36, 27 September 20122012-09-27T03:36:43Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:36, 27 September 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 33:</td>
<td colspan="2" class="diff-lineno">Line 33:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href:"https://static.igem.org/mediawiki/2012/6/6b/Air_Pump_Cris.jpg"><img src="https://static.igem.org/mediawiki/2012/6/6b/Air_Pump_Cris.jpg" widht="420" height="380"></a></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href:"https://static.igem.org/mediawiki/2012/6/6b/Air_Pump_Cris.jpg"><img src="https://static.igem.org/mediawiki/2012/6/6b/Air_Pump_Cris.jpg" widht="420" height="380"></a></center></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><center><b>Figure 1:</b> Diagram of the Co<sub>2</sub> pump.</center></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 42:</td>
<td colspan="2" class="diff-lineno">Line 43:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Fresh yeast is a living organism, a micro fungus able to perform fermentation: in absence of oxygen converts the sugar into alcohol and liberates CO<sub>2</sub>.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Fresh yeast is a living organism, a micro fungus able to perform fermentation: in absence of oxygen converts the sugar into alcohol and liberates CO<sub>2</sub>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>At last, pour 1 litre of Methylene blue into another bottle, used as a contamination control device, in order to purify the air that got into our culture. And finally we connect the air pumps to keep <del class="diffchange diffchange-inline">shaking </del>the <del class="diffchange diffchange-inline">coccus</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>At last, pour 1 litre of Methylene blue into another bottle, used as a contamination control device, in order to purify the air that got into our culture. And finally we connect the air pumps to keep <ins class="diffchange diffchange-inline">a turbulence regine inside </ins>the <ins class="diffchange diffchange-inline">bioreactor</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href:"https://static.igem.org/mediawiki/2012/3/3f/Air_Pump1_Cris.jpg"><img src="https://static.igem.org/mediawiki/2012/3/3f/Air_Pump1_Cris.jpg" width="420" height="380"></a></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href:"https://static.igem.org/mediawiki/2012/3/3f/Air_Pump1_Cris.jpg"><img src="https://static.igem.org/mediawiki/2012/3/3f/Air_Pump1_Cris.jpg" width="420" height="380"></a></center></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><center><b>Figure 2:</b>Photograph of the actual CO<sub>2</sub> pump we build.</center></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The results of our experiment were not the expected, having proved that our cyanobacteria grew up faster if we provided an extra input of CO<sub>2</sub>,bu the culture didn’t develop well due to contamination problems. We do not know if the yeast caused the problem or the bottle just wasn’t properly sealed.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The results of our experiment were not the expected, having proved that our cyanobacteria grew up faster if we provided an extra input of CO<sub>2</sub>,bu the culture didn’t develop well due to contamination problems. We do not know if the yeast caused the problem or the bottle just wasn’t properly sealed.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">b</del>>Air Pump</<del class="diffchange diffchange-inline">b</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">h3</ins>>Air Pump</<ins class="diffchange diffchange-inline">h3</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td colspan="2" class="diff-lineno">Line 56:</td>
<td colspan="2" class="diff-lineno">Line 58:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To avoid the contamination problems that happened at the previous design, we attached a clarification filter of 0.45micron pore size to retain the microorganisms from the incoming air flow.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To avoid the contamination problems that happened at the previous design, we attached a clarification filter of 0.45micron pore size to retain the microorganisms from the incoming air flow.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2012/a/a5/Imagenairpump.JPG"><img src="https://static.igem.org/mediawiki/2012/a/a5/Imagenairpump.JPG" width="450" height="400"></a></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2012/a/a5/Imagenairpump.JPG"><img src="https://static.igem.org/mediawiki/2012/a/a5/Imagenairpump.JPG" width="450" height="400"></a></center></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><center><b>Figure 3:</b> <i>Synechococcus</i> culture growing with the air pump</center></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To enhance better gas diffusion from the bubbled air, we built a bubble-diffusor stone rod at the end of the input tube, see below.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To enhance better gas diffusion from the bubbled air, we built a bubble-diffusor stone rod at the end of the input tube, see below.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2012/4/43/Piedraaaa.png"><img src="https://static.igem.org/mediawiki/2012/4/43/Piedraaaa.png"width="410" height="420"></a></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2012/4/43/Piedraaaa.png"><img src="https://static.igem.org/mediawiki/2012/4/43/Piedraaaa.png"width="410" height="420"></a></center></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><center><b>Figure 4:</b> Bubble-diffusor stone rod working.</center></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
</table>Gonzalohttp://2012.igem.org/wiki/index.php?title=Team:Valencia/Engineering&diff=236748&oldid=prevIrewija at 03:31, 27 September 20122012-09-27T03:31:33Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:31, 27 September 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 89:</td>
<td colspan="2" class="diff-lineno">Line 89:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Routine analyses of the system would be carried out on sucrose, oxygen and AHL levels in the liquid medium. Luminescence would be measured at night to acquire an experimental Light/Time curve.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Routine analyses of the system would be carried out on sucrose, oxygen and AHL levels in the liquid medium. Luminescence would be measured at night to acquire an experimental Light/Time curve.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2012/8/8b/Botella.jpg"> <img src="https://static.igem.org/mediawiki/2012/b/bd/IMG_0414.JPG"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><img src="https://static.igem.org/mediawiki/2012/8/8b/Botella.jpg<ins class="diffchange diffchange-inline">" width="120" heigth="100</ins>"> <img src="https://static.igem.org/mediawiki/2012/b/bd/IMG_0414.JPG<ins class="diffchange diffchange-inline">" width="200" height="200</ins>"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></center></div></td></tr>
</table>Irewijahttp://2012.igem.org/wiki/index.php?title=Team:Valencia/Engineering&diff=235986&oldid=prevIrewija at 03:19, 27 September 20122012-09-27T03:19:44Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 03:19, 27 September 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 88:</td>
<td colspan="2" class="diff-lineno">Line 88:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Routine analyses of the system would be carried out on sucrose, oxygen and AHL levels in the liquid medium. Luminescence would be measured at night to acquire an experimental Light/Time curve.<br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Routine analyses of the system would be carried out on sucrose, oxygen and AHL levels in the liquid medium. Luminescence would be measured at night to acquire an experimental Light/Time curve.<br><br></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><center></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><img src="https://static.igem.org/mediawiki/2012/8/8b/Botella.jpg"> <img src="https://static.igem.org/mediawiki/2012/b/bd/IMG_0414.JPG"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></center></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In our dry lab we tried different pumping and membrane systems which would ensure fast fluid exchange between the individual culture volumes without fast saturation of the membrane with bacterial mass. We ended up building a balanced flow circulation setting with relatively large-size pore membrane (0.45micron cellulose clarification filter modules) which still had a high saturation rate, near 1 minute. This gives us an estimate of the required rate of flow direction reversal we have to program in the microcontroller.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In our dry lab we tried different pumping and membrane systems which would ensure fast fluid exchange between the individual culture volumes without fast saturation of the membrane with bacterial mass. We ended up building a balanced flow circulation setting with relatively large-size pore membrane (0.45micron cellulose clarification filter modules) which still had a high saturation rate, near 1 minute. This gives us an estimate of the required rate of flow direction reversal we have to program in the microcontroller.</div></td></tr>
</table>Irewijahttp://2012.igem.org/wiki/index.php?title=Team:Valencia/Engineering&diff=234223&oldid=prevGonzalo at 02:47, 27 September 20122012-09-27T02:47:39Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:47, 27 September 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 57:</td>
<td colspan="2" class="diff-lineno">Line 57:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2012/a/a5/Imagenairpump.JPG"><img src="https://static.igem.org/mediawiki/2012/a/a5/Imagenairpump.JPG" width="450" height="400"></a></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2012/a/a5/Imagenairpump.JPG"><img src="https://static.igem.org/mediawiki/2012/a/a5/Imagenairpump.JPG" width="450" height="400"></a></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>To enhance better gas diffusion from the bubbled air, we built a bubble-diffusor stone rod at the end of the input tube <del class="diffchange diffchange-inline">(Fig 2)</del>.<br><br></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>To enhance better gas diffusion from the bubbled air, we built a bubble-diffusor stone rod at the end of the input tube<ins class="diffchange diffchange-inline">, see below</ins>.<br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2012/4/43/Piedraaaa.png"><img src="https://static.igem.org/mediawiki/2012/4/43/Piedraaaa.png"width="410" height="420"></a></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><a href="https://static.igem.org/mediawiki/2012/4/43/Piedraaaa.png"><img src="https://static.igem.org/mediawiki/2012/4/43/Piedraaaa.png"width="410" height="420"></a></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
</table>Gonzalohttp://2012.igem.org/wiki/index.php?title=Team:Valencia/Engineering&diff=232772&oldid=prevGonzalo at 02:20, 27 September 20122012-09-27T02:20:02Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:20, 27 September 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 11:</td>
<td colspan="2" class="diff-lineno">Line 11:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h2><b>Simple Batch Bioreactors</b></h2></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h2><b>Simple Batch Bioreactors</b></h2<ins class="diffchange diffchange-inline">><br><br</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b><del class="diffchange diffchange-inline">CO2 </del>Pump</b></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b><ins class="diffchange diffchange-inline">CO<sub>2</sub> </ins>Pump</b></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Gonzalohttp://2012.igem.org/wiki/index.php?title=Team:Valencia/Engineering&diff=232644&oldid=prevGonzalo at 02:17, 27 September 20122012-09-27T02:17:49Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:17, 27 September 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 101:</td>
<td colspan="2" class="diff-lineno">Line 101:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></center></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><h2><b>Measuring sucrose of the cscB</b></h2><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The cscB Synechococcus strain is capable to export sucrose at a concentration of 36mg/lh (Ducat et al. 2012). In order to confirm this in the cscB strain we were growing in the lab we designed two different methods:<br><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><ol></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><li>Measuring glucose with Fehling reactive.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><li>Measuring glucose with a standard glucometer.</li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ol></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">For the transformation of saccharose into glucose an invertase enzyme was needed which we couldn´t obtain. We will try to get it before the Jamboree to confirm the data of the Ducat et al. 2012 paper.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></html></div></td></tr>
</table>Gonzalohttp://2012.igem.org/wiki/index.php?title=Team:Valencia/Engineering&diff=231693&oldid=prevGonzalo at 01:58, 27 September 20122012-09-27T01:58:01Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:58, 27 September 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 64:</td>
<td colspan="2" class="diff-lineno">Line 64:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><br><img align="center" src="https://static.igem.org/mediawiki/2012/2/20/IMG_0399.JPG" width="220" higth="170" border="10"></a></center></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><center><br><img align="center" src="https://static.igem.org/mediawiki/2012/2/20/IMG_0399.JPG" width="220" higth="170" border="10"></a></center></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><br><br<del class="diffchange diffchange-inline">><br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><div id="HomeCenterCenter"</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We designed and built a simple biphasic coculture bioreactor prototype, easy to transport and operate, though not stable for long-term functioning. The construction is composed of 2 flasks of 10ml connected through a 0.45micron pore membrane from a clarification syringe filter. All joints were sealed with parafilm. The bioreactor is airtight, so oxygen and carbon dioxide levels would deplete with growing biomass if the proportions of each culture (autotrophic/heterotrophic) are not in metabolic equilibrium.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We designed and built a simple biphasic coculture bioreactor prototype, easy to transport and operate, though not stable for long-term functioning. The construction is composed of 2 flasks of 10ml connected through a 0.45micron pore membrane from a clarification syringe filter. All joints were sealed with parafilm. The bioreactor is airtight, so oxygen and carbon dioxide levels would deplete with growing biomass if the proportions of each culture (autotrophic/heterotrophic) are not in metabolic equilibrium.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br><br></div></td></tr>
</table>Gonzalo