Team:Utah State/Results

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         GFP Expression
         GFP Expression
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Green fluorescent protein (GFP) has been used by various iGEM teams to demonstrate expression and functionality of a BioBrick system. In order to demonstrate that the expression of a BioBrick spider silk gene (F1) is possible in E.coli a single spider silk gene was tagged with GFP at the C terminus to demonstrate silk protein protein expression. The GFP that was chosen for this study was taken from Utah State iGEM 2009 (BBa_K208000, http://partsregistry.org/wiki/index.php?title=Part:BBa_K208000) as it demonstrated high levels of GFP expression. This GFP protein has an excitation wavelength of 395nm and an emission wavelength of 509nm. The lac promoter and ribosome binding site used in this system was also taken from Utah State iGEM 2009 (BBa_K208010, http://partsregistry.org/wiki/index.php?title=Part:BBa_K208010). A plasmid map demonstrating this construct is shown below and this plasmid was transformed into DH5α.
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Green fluorescent protein (GFP) has been used by various iGEM teams to demonstrate expression and functionality of a BioBrick system. In order to demonstrate that the expression of a BioBrick spider silk gene (F1) is possible in E.coli a single spider silk gene was tagged with GFP at the C terminus to demonstrate silk protein protein expression. The GFP that was chosen for this study was taken from Utah State iGEM 2009 (<a href= "http://partsregistry.org/wiki/index.php?title=Part:BBa_K208000"> BBa_K208000 </a>) as it demonstrated high levels of GFP expression. This GFP protein has an excitation wavelength of 395nm and an emission wavelength of 509nm. The lac promoter and ribosome binding site used in this system was also taken from Utah State iGEM 2009 (BBa_K208010, http://partsregistry.org/wiki/index.php?title=Part:BBa_K208010). A plasmid map demonstrating this construct is shown below and this plasmid was transformed into DH5α.

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