Team:Utah State/Results

From 2012.igem.org

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<div style="position: absolute; top: 3520px;"><img src="https://static.igem.org/mediawiki/2012/8/88/Wheresspidey2.png" onMouseOver="this.src='https://static.igem.org/mediawiki/2012/9/92/SpiderFacts7.png'" onMouseOut="this.src='https://static.igem.org/mediawiki/2012/8/88/Wheresspidey2.png'"></div>
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</h1></a>
</h1></a>
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<p align="center"><img src="https://static.igem.org/mediawiki/2012/2/28/USU_HowOurSystemWorks.PNG"></p>
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<p align="center"><img src="https://static.igem.org/mediawiki/2012/b/b6/USU_HowOurSystemWorks2.PNG"></p>
<br><a name="FavoriteParts"><h1 style="width: 100%; margin-left: 0px; margin-right: 20px; text-align: left; font-family:Arial, Helvetica, sans-serif; font-weight: normal; border-bottom: 2px solid #C0C0C0;" class="table-main">
<br><a name="FavoriteParts"><h1 style="width: 100%; margin-left: 0px; margin-right: 20px; text-align: left; font-family:Arial, Helvetica, sans-serif; font-weight: normal; border-bottom: 2px solid #C0C0C0;" class="table-main">
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<p class="textProfile">
<p class="textProfile">
<ol>
<ol>
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<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K844000">Main page</a> - <b>10x Histidine (10x His), Tag with double stop codon, BBa_K844000</b>: This part is fully functional as spider silk can be purified using a Nickel column.
 
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K844016">Main page</a> - <b>Spider Silk Generator, BBa_K844016</b>: Contains a lac/IPTG promoter and 4x spider silk unit and a His-tag for purification. This part is fully functional (proved by protein gel) and is able to generate spider silk.
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K844016">Main page</a> - <b>Spider Silk Generator, BBa_K844016</b>: Contains a lac/IPTG promoter and 4x spider silk unit and a His-tag for purification. This part is fully functional (proved by protein gel) and is able to generate spider silk.
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<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K844015">Main page</a> - <b>lac/IPTG inducible Spider Silk 1x “F” Subunit fused GFP, BBa_K844015</b>: This part is a fully functional spider silk-GFP fusion.
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<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K844000">Main page</a> - <b>10x Histidine (10x His)-Tag with double stop codon, BBa_K844000</b>: This part is fully functional as spider silk can be purified using a nickel column.
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<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K844015">Main page</a> - <b>lac/IPTG inducible Spider Silk 1x “F” Subunit fused GFP, BBa_K844015</b>: This part is a fully functional spider silk-GFP fusion protein as demonstrated by GFP flourescence.
</ol>
</ol>
</p>
</p>
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<ol>
<ol>
<li><a href="http://partsregistry.org/Part:BBa_K208010:Experience#User_Reviews
<li><a href="http://partsregistry.org/Part:BBa_K208010:Experience#User_Reviews
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">Experience</a> – <b>BBa_K208010, Lac Promoter and RBS</b> (Utah State iGEM 2009). This part was successfully able express of spider silk and spider silk-GFP fusion protein.
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">Experience</a> – <b>BBa_K208010, Lac Promoter and RBS</b> (Utah State iGEM 2009): This part was successfully able express of spider silk and spider silk-GFP fusion protein.
<li><a href="http://partsregistry.org/Part:BBa_K208000:Experience#User_Reviews  
<li><a href="http://partsregistry.org/Part:BBa_K208000:Experience#User_Reviews  
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">Experience</a> – <b>BBa_K208000, GFP</b>. This part was successfully fused to a “F” spider silk subunit and was also successfully expressed.
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">Experience</a> – <b>BBa_K208000, GFP</b> (Utah State iGEM 2009): This part was successfully fused to a “F” spider silk subunit and was also successfully expressed.
</ol>
</ol>
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<p class="textProfile">
<p class="textProfile">
<ol>
<ol>
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<li><a href="http://partsregistry.org/Part:BBa_K208008">Experience</a> - <b>Spider Silk 1x Subunit “B” (Balanced tRNA codon optimized) with Met (ATG) added, BBa_K844008</b>. Part works in Bba_K844016.
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<li><a href="http://partsregistry.org/Part:BBa_K844008">Main page</a> - <b>Spider Silk 1x Subunit “B” (Balanced tRNA codon optimized) with Met (ATG) added, BBa_K844008</b> : Part works in BBa_K844016.
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<li><a href="http://partsregistry.org/Part:BBa_K208004">Experience</a> - <b>Spider silk 3x Subunit “B” (Balanced tRNA codon optimized), Bba_K844004. Works in BBa_K844016</b>. This part is optimized based on the native “W” construct, BBa_K844002.
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<li><a href="http://partsregistry.org/Part:BBa_K844004">Main page</a> - <b>Spider silk 3x Subunit “B” (Balanced tRNA codon optimized), BBa_K844004:</b> Works in BBa_K844016. This part is optimized based on the native “W” construct, BBa_K844002.
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<li><a href=http://partsregistry.org/Part:BBa_K20807">Experience</a> – <b>Spider silk 1x Subunit “F” (Fewest tRNA codon optimized) with Met (ATG) added, BBa_K844007</b>. Works in BBa_K844015. This part is optimized based on native “W” construct, BBa_K844002.
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<li><a href="http://partsregistry.org/Part:BBa_K844007">Main page</a> – <b>Spider silk 1x Subunit “F” (Fewest tRNA codon optimized) with Met (ATG) added, BBa_K844007</b> : Works in BBa_K844015. This part is optimized based on native “W” construct, BBa_K844002.
</ol>
</ol>
</p>
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<a name="Cloning"><h1 style="width: 100%; margin-left: 0px; margin-right: 20px; text-align: left; font-family:Arial, Helvetica, sans-serif; font-weight: normal; border-bottom: 2px solid #000000;" class="table-main">
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         Cloning
         Cloning
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<p align="center"><img src="https://static.igem.org/mediawiki/2012/a/ae/Patgb4ht_silk_sample.PNG" alt="usu_silk" width="500" height="287"></p>
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<div style="display: inline-block;"><p style="float: left;"><img src="https://static.igem.org/mediawiki/2012/a/ae/Patgb4ht_silk_sample.PNG" alt="usu_silk" width="500px" height="287px"></p><p style="float: right;"><img src="https://static.igem.org/mediawiki/2012/2/2c/Newsilk.jpg" alt="usu_silk" width="500px" height="373px"></p></div>

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