Team:Uppsala University/Team

From 2012.igem.org

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<div id="headertext">Team</div>
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<div id="headertext">New standard backbones</div>
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<a href="#supervisors">Supervisors</a> | <a href="#instructors">Instructors</a> | <a href="#students">Team members</a> | <a href="#advisors">Advisors</a> | <a href="#contact">Contact us</a>
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<a href="#standard">Standard</a> | <a href="#laciq">LaqIq</a> | <a href="#flp">Flp</a>
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<img src="https://static.igem.org/mediawiki/2012/5/53/Group.jpg" width="60%" alt="Team Uppsala University"><br>
 
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<i>Team Uppsala University 2012 (some members missing)</i>
 
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<tr><td class="subtext"><h2>Supervisors</h2></td>
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<td class="subtext"><h2>Standard backbones</h2></td>
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<td valign="bottom"><a id="top" href="#top">Back to top</a></td></tr>
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<img src="https://static.igem.org/mediawiki/2012/6/69/AnthonyForster.png" alt="Tony"><br>
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<b>Prof. <br> Anthony Forster</b>
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<b>Prof. <br> Anders Virtanen</b>
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Anthony Forster is a professor at the Department of Cell and Molecular Biology and a member of the Uppsala RNA Research Centre (URRC). He is researching synthetic biology, protein synthesis and drug discovery, and is currently working towards the synthesis of a minimal cell. <a href="http://openwetware.org/wiki/Forster_Lab/">Read more...</a>
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Anders Virtanen is a professor at the Department of Cell and Molecular Biology and a member of the Uppsala RNA Research Centre (URRC). Anders studies primarily enzymes involved in regulating the fate of mRNA. One of his goals is to identify previously unrecognized small non-coding RNAs (ncRNA) and to determine their functions. <a href="http://www2.icm.uu.se/molbio/virtanen/">Read more...</a>
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<td width="100"><b>Registry ID</b></td><td width="100"><b>Name</b></td> <td width="100"><b>Ori<b> </td> <td width="100"><b>Resistance</b></td> <td width="100"><b>Insert</b></td> <td width="100"><b>Status</b></td>
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<td></td><td>pSB4A15</td> <td>pSC101</td><td>Amp</td><td>pUC-red</td><td>Finished</td>
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<td></td><td>pSB4C15</td> <td>pSC101</td><td>Cm</td><td>pUC-red</td><td>Finished</td>
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<td></td><td>pSB4K15</td> <td>pSC101</td><td>Kan</td><td>pUC-red</td><td>Planning</td>
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<td></td><td>pSB4S15</td> <td>pSC101</td><td>Spec</td><td>pUC-red</td><td>Finished</td>
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<td></td><td>pSB4T15</td> <td>pSC101</td><td>Tet</td><td>pUC-red</td><td>Finished</td>
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<tr><td class="subtext"><h2>Instructors</h2></td>
 
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<td valign="bottom"><a id="top" href="#top">Back to top</a></td></tr>
 
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<img src="https://static.igem.org/mediawiki/2012/1/13/ErikGullberg.jpg" alt="Gullan"><br>
 
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<b>Erik Gullberg</b><br>
 
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<img src="https://static.igem.org/mediawiki/2012/f/f9/MickeNissbeck.jpg" alt="Micke"><br>
 
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<b>Mikael Nissbeck</b>
 
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PhD student in Medical Bacteriology at the Department of Medical Biochemistry and Microbiology at Uppsala University since 2009 and is currently working on antibiotic resistance in bacteria in the Dan Andersson group at the Uppsala Biomedical Center. So far he has published articles in PLoS Pathogens and Molecular Microbiology.
 
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PhD student at Uppsala RNA Research Center (URRC), Department of Cell and Molecular biology, at Uppsala University since 2012 and is currently working on the human enzyme Poly(A)-specific ribonuclease (PARN) in bacteria in the Anders Virtanen group at the Uppsala Biomedical Center.
 
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<td class="subtext"><h2>LaqIq backbones</h2></td>
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<tr><td class="subtext"><h2>Team members</h2></td>
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<td valign="bottom"><a id="top" href="#top">Back to top</a></td></tr>
<td valign="bottom"><a id="top" href="#top">Back to top</a></td></tr>
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<h3>Headquarter</h3>
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</td>
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For expression of toxic genes, or simply genes where you want to be able to tune the expression level, we constructed a series of lacIq bacbones. Including the lacIq casette on the plasmid ensures that the copy number of the repression always follows that of your inserted genes, providing guranteed strong repression without inducing unneccessary metabolic load.  
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<img src="https://static.igem.org/mediawiki/2012/4/44/ErikLundin.jpg" alt="Teamleader"><br>
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<b>Erik - Team leader</b>
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<img src="https://static.igem.org/mediawiki/2012/d/d4/PikkeiYuen.jpg" alt="Assistant"><br>
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<b>Pikkei - assistant</b>
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<td width="100"><b>Registry ID</b></td><td width="100"><b>Name</b></td> <td width="100"><b>Ori<b> </td> <td width="100"><b>Resistance</b></td> <td width="100"><b>Insert</b></td> <td width="100"><b>Status</b></td>
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<h3>Reporter- and screeningsystem group</h3>
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</tr><tr>
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</td>
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<td></td><td>pSB4A15Iq</td> <td>pSC101</td><td>Amp</td><td>pUC-red</td><td>Planning</td>
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</tr><tr>
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<td></td><td>pSB4C15Iq</td> <td>pSC101</td><td>Cm</td><td>pUC-red</td><td>Planning</td>
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</tr><tr>
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<td></td><td>pSB4K15Iq</td> <td>pSC101</td><td>Kan</td><td>pUC-red</td><td>Planning</td>
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</tr><tr>
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<td></td><td>pSB4S15Iq</td> <td>pSC101</td><td>Spec</td><td>pUC-red</td><td>Planning</td>
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</tr><tr>
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<td></td><td>pSB4T15Iq</td> <td>pSC101</td><td>Tet</td><td>pUC-red</td><td>Planning</td>
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<td colspan="2" id="group">
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<img src="https://static.igem.org/mediawiki/2012/0/0b/GroupA.jpg" width="500" alt="Group A"><br>
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<b>Joel, Caroline, Emy, Katarina</b>
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<h3>Gene networks group</h3>
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<td colspan="2" id="group">
 
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<img src="https://static.igem.org/mediawiki/2012/2/28/GruppB.jpg" width="500" alt="Group B"><br>
 
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<b>Anders, Josefin & Niklas</b>
 
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<h3>TALEN group</h3>
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<img src="https://static.igem.org/mediawiki/2012/3/37/GroupC.jpg" width="500" alt="Group C"><br>
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<b>Joakim, Arvid, Anders & Anton</b>
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</td>
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<h3>Conjugative vector design group</h3>
 
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<td colspan="2" id="group">
 
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<img src="https://static.igem.org/mediawiki/2012/3/3a/GroupD.jpg" width="500" alt="Group D"><br>
 
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<b>Fredrik, Anna & Joakim</b>
 
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</td>
 
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</tr>
 
<tr>
<tr>
<td colspan="2">
<td colspan="2">
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<h3>Randomized sRNA group</h3>
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<a name="flp"></a>
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</td>
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<table>
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</tr>
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<td colspan="2" id="group">
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<img src="https://static.igem.org/mediawiki/2012/a/ad/GroupE.jpg" width="500" alt="Group E"><br>
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<b>Tobias, Sabri, Hampus, Julia, Lisa & Christofer</b>
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</td>
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<td colspan="2">
 
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<a name="advisors"></a>
 
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<td class="subtext"><h2>LaqIq backbones</h2></td>
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<td colspan="2">
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<table>
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<tr><td class="subtext"><h2>Advisors</h2></td>
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<td valign="bottom"><a id="top" href="#top">Back to top</a></td></tr>
<td valign="bottom"><a id="top" href="#top">Back to top</a></td></tr>
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We have made special low copy backbones for recombination onto the bacterial chromosome. The antibiotic resistance casette is flanked by Flp recombinase recognition target sites (FRT sites), meaning that the casette can be flipped out of the chromosome after integration and selection. Since low copy plasmids still have a higher copy number than one, it can be also useful to to repress toxic genes during cloning work with the lacIq system.
<table>
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<td width="100"><b>Registry ID</b></td><td width="100"><b>Name</b></td> <td width="100"><b>Ori<b> </td> <td width="100"><b>Resistance</b></td> <td width="100"><b>Insert</b></td> <td width="100"><b>Status</b></td>
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<img src="https://static.igem.org/mediawiki/2012/3/38/Erik_H_bild.jpg" alt="Erik H"><br>
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<b>Erik Holmqvist</b>
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<td></td><td>pSB4A15Flp</td> <td>pSC101</td><td>Amp</td><td>pUC-red</td><td>Finished</td>
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<td></td><td>pSB4C15Flp</td> <td>pSC101</td><td>Cm</td><td>pUC-red</td><td>Finished</td>
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<img src="https://static.igem.org/mediawiki/2012/4/4b/HerveNicoloff.png" alt="Hervé"><br>
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<b>Hervé Nicoloff</b>
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<td></td><td>pSB4K15Flp</td> <td>pSC101</td><td>Kan</td><td>pUC-red</td><td>Finished</td>
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</td>
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<td></td><td>pSB4S15Flp</td> <td>pSC101</td><td>Spec</td><td>pUC-red</td><td>Finished</td>
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<img src="" alt="Lars"><br>
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<b>Lars Arvidsson</b>
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<td></td><td>pSB4T15Flp</td> <td>pSC101</td><td>Tet</td><td>pUC-red</td><td>Finished</td>
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<td></td><td>pSB4A15FlpIq</td> <td>pSC101</td><td>Amp</td><td>pUC-red</td><td>Planning</td>
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<img src="https://static.igem.org/mediawiki/2012/5/5f/DanielCamsund.png" alt="Daniel"><br>
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<b>Daniel Camsund</b>
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<td></td><td>pSB4C15FlpIq</td> <td>pSC101</td><td>Cm</td><td>pUC-red</td><td>Planning</td>
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<td></td><td>pSB4K15FlpIq</td> <td>pSC101</td><td>Kan</td><td>pUC-red</td><td>Planning</td>
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<td></td><td>pSB4S15FlpIq</td> <td>pSC101</td><td>Spec</td><td>pUC-red</td><td>Planning</td>
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<td></td><td>pSB4T15FlpIq</td> <td>pSC101</td><td>Tet</td><td>pUC-red</td><td>Planning</td>
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<td valign="bottom"><a id="top" href="#top">Back to top</a></td></tr>
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<tr><td colspan="2" style="padding: 0px 50px; width: 500px; text-align: center; white-space: normal;">
 
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Correspondance should be addressed electronically to <b>igemuppsala@gmail.com</b> by using the form below. Alternatively, you can click <a href="mailto:igemuppsala@gmail.com">here</a> to use your personal mail client.
 
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<input name="subject" type="hidden" id="subject" value="Message sent from Team UU website" />
 
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<input name="reset" type="reset" value="Reset"  />
 
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<tr><td colspan="2" style="text-align:center; font-size: 10px"><a href="http://www.mycontactform.com" target="_blank">Powered by myContactForm.com</a></td></tr>
 
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Revision as of 14:40, 10 September 2012

Team Uppsala University – iGEM 2012


Standard backbones

 Back to top

Registry IDName Ori Resistance Insert Status
pSB4A15 pSC101AmppUC-redFinished
pSB4C15 pSC101CmpUC-redFinished
pSB4K15 pSC101KanpUC-redPlanning
pSB4S15 pSC101SpecpUC-redFinished
pSB4T15 pSC101TetpUC-redFinished

LaqIq backbones

 Back to top

For expression of toxic genes, or simply genes where you want to be able to tune the expression level, we constructed a series of lacIq bacbones. Including the lacIq casette on the plasmid ensures that the copy number of the repression always follows that of your inserted genes, providing guranteed strong repression without inducing unneccessary metabolic load.

Registry IDName Ori Resistance Insert Status
pSB4A15Iq pSC101AmppUC-redPlanning
pSB4C15Iq pSC101CmpUC-redPlanning
pSB4K15Iq pSC101KanpUC-redPlanning
pSB4S15Iq pSC101SpecpUC-redPlanning
pSB4T15Iq pSC101TetpUC-redPlanning

LaqIq backbones

 Back to top

We have made special low copy backbones for recombination onto the bacterial chromosome. The antibiotic resistance casette is flanked by Flp recombinase recognition target sites (FRT sites), meaning that the casette can be flipped out of the chromosome after integration and selection. Since low copy plasmids still have a higher copy number than one, it can be also useful to to repress toxic genes during cloning work with the lacIq system.

Registry IDName Ori Resistance Insert Status
pSB4A15Flp pSC101AmppUC-redFinished
pSB4C15Flp pSC101CmpUC-redFinished
pSB4K15Flp pSC101KanpUC-redFinished
pSB4S15Flp pSC101SpecpUC-redFinished
pSB4T15Flp pSC101TetpUC-redFinished
pSB4A15FlpIq pSC101AmppUC-redPlanning
pSB4C15FlpIq pSC101CmpUC-redPlanning
pSB4K15FlpIq pSC101KanpUC-redPlanning
pSB4S15FlpIq pSC101SpecpUC-redPlanning
pSB4T15FlpIq pSC101TetpUC-redPlanning



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