Team:University College London/Protocols/T4dnaligase

From 2012.igem.org

Revision as of 20:15, 26 September 2012 by Aurelija (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Transformation Protocol 2

Nuclease positive cells

COMPONENT 20 μl REACTION)
10X T4 DNA Ligase Buffer 2 μl
Vector DNA (3 kb) 50 ng (0.025 pmol)
Insert DNA (1 kb 50 ng (0.076 pmol)
Nuclease-free water to 20 μl
T4 DNA Ligase 1 μl