Team:UT-Tokyo/Project/H2 E.coli/Results

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== Result ==
== Result ==
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We measured the amount of H₂ produced by E.coli transformed with either “pLac-RBS-fhlA-d.term”(fhlA) or “pLac-RBS-fhlA(E363G)-d.term”(E363G)  by the method written in the page [[Team:UT-Tokyo/LabWork/AssayMethods|AssayMethods]]. In this page, we describe the data.
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We measured the amount of H<sub>2</sub> produced by ''E.coli'' transformed with either “pLac-RBS-fhlA-d.term”(''fhlA'') or “pLac-RBS-fhlA(E363G)-d.term”(E363G)  by the method written in the page [[Team:UT-Tokyo/LabWork/AssayMethods|AssayMethods]]. In this page, we describe the data.
The result is  the following (Fig.1)<br />
The result is  the following (Fig.1)<br />
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[[file:UT Tokyo Fig diagram 640 480.png]]
[[file:UT Tokyo Fig diagram 640 480.png]]
   
   
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Fig.1:H₂production in liquid LB medium containing the labeled glucose concentration.<br />
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Fig.1:H<sub>2</sub>production in liquid LB medium containing the labeled glucose concentration.<br />
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The vertical axis expresses v/v% of H₂ in the gaseous phase after we cultivate each E.coli for 8 hours in a container with 1mL each of LB medium and the gaseous phase .The horizontal axis shows the kinds of E.coli and the concentrations of glucose in each medium(“LB+x%” means the glucose w/v concentrations of liquid LB medium is x% more than the usual medium.<br />
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The vertical axis expresses v/v% of H<sub>2</sub> in the gaseous phase after we cultivate each ''E.coli'' for 8 hours in a container with 1mL each of LB medium and the gaseous phase .The horizontal axis shows the kinds of ''E.coli'' and the concentrations of glucose in each medium(“LB+x%” means the glucose w/v concentrations of liquid LB medium is x% more than the usual medium.<br />
The results shown by the Fig.1 is statistically sufficient to conclude the followings.<br />
The results shown by the Fig.1 is statistically sufficient to conclude the followings.<br />
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*The amount of H₂ production is fhlA>fhlA(E363G)>wild type in these conditions.
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*The amount of H<sub>2</sub> production is ''fhlA'' > ''fhlA''(E363G) > wild type in these conditions.
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*There is a positive correlation between the amount of H₂ produced by fhlA and the glucose concentration in the liquid LB.
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*There is a positive correlation between the amount of H<sub>2</sub> produced by ''fhlA'' and the glucose concentration in the liquid LB.
   
   
The detailed interpretations of this result are done in the [[Team:UT-Tokyo/Project/H2_E.coli/Discussion|Discussion]].
The detailed interpretations of this result are done in the [[Team:UT-Tokyo/Project/H2_E.coli/Discussion|Discussion]].

Revision as of 22:54, 26 September 2012

H2 E.coli: Results

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このページの概要を、簡単に記述。

Result

We measured the amount of H2 produced by E.coli transformed with either “pLac-RBS-fhlA-d.term”(fhlA) or “pLac-RBS-fhlA(E363G)-d.term”(E363G) by the method written in the page AssayMethods. In this page, we describe the data.

The result is the following (Fig.1)

UT Tokyo Fig diagram 640 480.png

Fig.1:H2production in liquid LB medium containing the labeled glucose concentration.
The vertical axis expresses v/v% of H2 in the gaseous phase after we cultivate each E.coli for 8 hours in a container with 1mL each of LB medium and the gaseous phase .The horizontal axis shows the kinds of E.coli and the concentrations of glucose in each medium(“LB+x%” means the glucose w/v concentrations of liquid LB medium is x% more than the usual medium.

The results shown by the Fig.1 is statistically sufficient to conclude the followings.

  • The amount of H2 production is fhlA > fhlA(E363G) > wild type in these conditions.
  • There is a positive correlation between the amount of H2 produced by fhlA and the glucose concentration in the liquid LB.

The detailed interpretations of this result are done in the Discussion.