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Team:UT-Tokyo/Project/H2 E.coli/Results
From 2012.igem.org
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| - | == | + | == Result == |
We measured the amount of H₂ produced by each E.coli transducted “pLac-RBS-fhlA-d.term”(fhlA) or “pLac-RBS-fhlA(E363G)-d.term”(E363G) with wild type by the method written in the page [[Team:UT-Tokyo/LabWork/AssayMethods|AssayMethods]].<br /> | We measured the amount of H₂ produced by each E.coli transducted “pLac-RBS-fhlA-d.term”(fhlA) or “pLac-RBS-fhlA(E363G)-d.term”(E363G) with wild type by the method written in the page [[Team:UT-Tokyo/LabWork/AssayMethods|AssayMethods]].<br /> | ||
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The results shown by the Fig.1 is statistically sufficient to conclude the followings.<br /> | The results shown by the Fig.1 is statistically sufficient to conclude the followings.<br /> | ||
| - | + | *The amount of H₂ production is fhlA>fhlA(E363G)>wild type in the same situations.<br /> | |
| - | + | *There is a positive correlation between the amount of H₂ produced by fhlA and the glucose concentration of the liquid LB concentration. | |
The detailed interpretations of this result are done in the [[Team:UT-Tokyo/Project/H2_E.coli/Discussion|Discussion]]. | The detailed interpretations of this result are done in the [[Team:UT-Tokyo/Project/H2_E.coli/Discussion|Discussion]]. | ||
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Revision as of 22:36, 26 September 2012
H2 E.coli: Results
このページの概要を、簡単に記述。
Result
We measured the amount of H₂ produced by each E.coli transducted “pLac-RBS-fhlA-d.term”(fhlA) or “pLac-RBS-fhlA(E363G)-d.term”(E363G) with wild type by the method written in the page AssayMethods.
In this page, we describe the date.
The result is as the following graph(Fig.1)
Fig.1:H₂production in liquid LB medium of each glucose concentration.
This vertical axis expresses v/v% of H₂ per the gaseous phase after we cultivate each E.coli for 8 hour in the situation the liquid LB medium and the gaseous phase is 1mL.And this horizontal one shows that the kinds of E.coli and the concentrations of glucose of each medium(“LB+x%” means the glucose w/v concentrations of liquid LB medium is x% more than the usual medium.
The results shown by the Fig.1 is statistically sufficient to conclude the followings.
- The amount of H₂ production is fhlA>fhlA(E363G)>wild type in the same situations.
- There is a positive correlation between the amount of H₂ produced by fhlA and the glucose concentration of the liquid LB concentration.
The detailed interpretations of this result are done in the Discussion.
