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Team:UT-Tokyo/LabWork/RegularMethods
From 2012.igem.org
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====Protocol==== | ====Protocol==== | ||
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===Ligation=== | ===Ligation=== | ||
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*Insert DNA | *Insert DNA | ||
=====2.Incubation at 16 °C for 15-30 min.===== | =====2.Incubation at 16 °C for 15-30 min.===== | ||
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| + | ==Transformation== | ||
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| + | ==Purification of DNA== | ||
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| + | ==Reagents== | ||
<!-- 以上自由記述 --> | <!-- 以上自由記述 --> | ||
Revision as of 21:44, 26 September 2012
Regular Methods
Assembly parts
Digest
Materials
Protocol
Ligation
Materials
- Vector DNA
- Insert DNA
- 2x Ligation Mix
Protocol
1.Make reaction liquid
- MilliQ up to 20uL
- 10uL 2x Ligation Mix
- Vector DNA
- Insert DNA
2.Incubation at 16 °C for 15-30 min.
Transformation
Purification of DNA
Reagents
