Team:USP-UNESP-Brazil/Plasmid Plug n Play/Introduction

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We intended to test three different plasmids: a low copy number plasmid (pSB4A5), a high copy number plasmid (pSB1C3) and a commercial high copy number plasmid (pET15b). The comparison of these three plasmids will identify the best system for the developing of this technology.  
We intended to test three different plasmids: a low copy number plasmid (pSB4A5), a high copy number plasmid (pSB1C3) and a commercial high copy number plasmid (pET15b). The comparison of these three plasmids will identify the best system for the developing of this technology.  
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Since the Cre expression levels are critical for the system performance, we created two different versions for each of the three plasmids, A (Cre-Lox71) and B (Lox71-Cre). We want to test if inserting the target gene either upstream or downstream the Cre gene will affect the expression efficiency.
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Since the Cre expression levels are critical for the system performance, we created two different versions for each of the three plasmids, A (lox71-Cre) and B (Cre-lox71). We want to test if inserting the target gene either upstream or downstream the Cre gene will affect the expression efficiency.

Revision as of 22:22, 24 September 2012