http://2012.igem.org/wiki/index.php?title=Team:USP-UNESP-Brazil/Associative_Memory/Experiments&feed=atom&action=historyTeam:USP-UNESP-Brazil/Associative Memory/Experiments - Revision history2024-03-28T10:17:56ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:USP-UNESP-Brazil/Associative_Memory/Experiments&diff=237843&oldid=prevOtto at 03:49, 27 September 20122012-09-27T03:49:08Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">=Planned Experiments=</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>At first, before doing assembles, we confirmed all parts that were to be used, by verifying the lengths of the inserts in the plasmids using agarose gel and digestion by enzymes that cut in the prefixes and suffixes of BioBricks(EcoRI and PstI).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>At first, before doing assembles, we confirmed all parts that were to be used, by verifying the lengths of the inserts in the plasmids using agarose gel and digestion by enzymes that cut in the prefixes and suffixes of BioBricks(EcoRI and PstI).</div></td></tr>
</table>Ottohttp://2012.igem.org/wiki/index.php?title=Team:USP-UNESP-Brazil/Associative_Memory/Experiments&diff=237075&oldid=prevChico at 03:37, 27 September 20122012-09-27T03:37:27Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">In order to build the genic design (shown on the introductory part), we used the BioBricks listed on Appendix 1.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>At first, before doing <ins class="diffchange diffchange-inline">assembles</ins>, we confirmed all parts that were to be used, by verifying the lengths of the inserts in the plasmids using agarose gel and digestion by enzymes that cut in the prefixes and suffixes of BioBricks(EcoRI and PstI).</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>At first, before doing <del class="diffchange diffchange-inline">assemblies</del>, we confirmed all parts that were to be used, by verifying the lengths of the inserts in the plasmids using agarose gel and digestion by enzymes that cut in the prefixes and suffixes of BioBricks(EcoRI and PstI).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Later, we assembled three constructions, each one for an experiment (a test) regarding the genetic device to be used in later constructions.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Later, we assembled three constructions, each one for an experiment (a test) regarding the genetic device to be used in later constructions.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Chicohttp://2012.igem.org/wiki/index.php?title=Team:USP-UNESP-Brazil/Associative_Memory/Experiments&diff=236260&oldid=prevChico at 03:23, 27 September 20122012-09-27T03:23:56Z<p></p>
<a href="http://2012.igem.org/wiki/index.php?title=Team:USP-UNESP-Brazil/Associative_Memory/Experiments&diff=236260&oldid=235119">Show changes</a>Chicohttp://2012.igem.org/wiki/index.php?title=Team:USP-UNESP-Brazil/Associative_Memory/Experiments&diff=235119&oldid=prevOtto at 03:04, 27 September 20122012-09-27T03:04:09Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">=Planned Experiments=</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In order to construct the genic design (shown on the introductory part), we Will use bio bricks listed on appendix 1.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In order to construct the genic design (shown on the introductory part), we Will use bio bricks listed on appendix 1.</div></td></tr>
</table>Ottohttp://2012.igem.org/wiki/index.php?title=Team:USP-UNESP-Brazil/Associative_Memory/Experiments&diff=184470&oldid=prevOtto at 01:30, 26 September 20122012-09-26T01:30:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We intend to assembly all biobricks using the 3A assembly method, except the smaller parts, like RBS and terminal sequences in which will be used the Standard Assembly Method. Depending on the method of assembly, a different scheme of digestion will be used. The different types of digestion are on the assembly fluxogram. Alternatively to the initial plan using the light switch, IPTG will be used as input to induce the initial production of GFP.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We intend to assembly all biobricks using the 3A assembly method, except the smaller parts, like RBS and terminal sequences in which will be used the Standard Assembly Method. Depending on the method of assembly, a different scheme of digestion will be used. The different types of digestion are on the assembly fluxogram. Alternatively to the initial plan using the light switch, IPTG will be used as input to induce the initial production of GFP.</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>{{:Team:USP-UNESP-Brazil/Templates/<del class="diffchange diffchange-inline">Header</del>}}</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>{{:Team:USP-UNESP-Brazil/Templates/<ins class="diffchange diffchange-inline">Foot</ins>}}</div></td></tr>
</table>Ottohttp://2012.igem.org/wiki/index.php?title=Team:USP-UNESP-Brazil/Associative_Memory/Experiments&diff=184466&oldid=prevOtto at 01:30, 26 September 20122012-09-26T01:30:17Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:USP-UNESP-Brazil/Templates/Header}}</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>{{:Team:USP-UNESP-Brazil/Templates/Header}}</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In order to construct the genic design (shown on the introductory part), we Will use bio bricks listed on appendix 1.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In order to construct the genic design (shown on the introductory part), we Will use bio bricks listed on appendix 1.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>At first, before doing assemblies, we intend to confirm all parts that will be used. We will verify the lengths of the inserts in the plasmids using agarose gel and digestion by enzymes that cut in the prefixes and suffixes of BioBricks(EcoRI and PstI).</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>At first, before doing assemblies, we intend to confirm all parts that will be used. We will verify the lengths of the inserts in the plasmids using agarose gel and digestion by enzymes that cut in the prefixes and suffixes of BioBricks(EcoRI and PstI).</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We intend to assembly all biobricks using the 3A assembly method, except the smaller parts, like RBS and terminal sequences in which will be used the Standard Assembly Method. Depending on the method of assembly, a different scheme of digestion will be used. The different types of digestion are on the assembly fluxogram. Alternatively to the initial plan using the light switch, IPTG will be used as input to induce the initial production of GFP.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We intend to assembly all biobricks using the 3A assembly method, except the smaller parts, like RBS and terminal sequences in which will be used the Standard Assembly Method. Depending on the method of assembly, a different scheme of digestion will be used. The different types of digestion are on the assembly fluxogram. Alternatively to the initial plan using the light switch, IPTG will be used as input to induce the initial production of GFP.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">{{:Team:USP-UNESP-Brazil/Templates/Header}}</ins></div></td></tr>
</table>Ottohttp://2012.igem.org/wiki/index.php?title=Team:USP-UNESP-Brazil/Associative_Memory/Experiments&diff=150417&oldid=prevPepeks: /* Assembly diagrams */2012-09-23T20:02:02Z<p><span class="autocomment">Assembly diagrams</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Previously, it was intent to use a light receptor (Red Light Suit) as input system. The strategy was to put the light responsive promoter in sequence to the Prm promoter, making the genic regulation of the following ORF. In order to simplify the Project, this part was removed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Previously, it was intent to use a light receptor (Red Light Suit) as input system. The strategy was to put the light responsive promoter in sequence to the Prm promoter, making the genic regulation of the following ORF. In order to simplify the Project, this part was removed.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>To illustrate all constructions used in all experiments, the Venn diagram ([https://static.igem.org/mediawiki/2012/2/2a/Assembly_Map.png HERE]) was created, gathering all partial constructions in the main construction. <del class="diffchange diffchange-inline">In this LINK, there </del>is the assembly fluxogram.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>To illustrate all constructions used in all experiments, the Venn diagram ([https://static.igem.org/mediawiki/2012/2/2a/Assembly_Map.png HERE]) was created, gathering all partial constructions in the main construction. <ins class="diffchange diffchange-inline">[https://static.igem.org/mediawiki/2012/b/b3/Assemblys_in_english_full.jpg HERE] </ins>is the assembly fluxogram.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We intend to assembly all biobricks using the 3A assembly method, except the smaller parts, like RBS and terminal sequences in which will be used the Standard Assembly Method. Depending on the method of assembly, a different scheme of digestion will be used. The different types of digestion are on the assembly fluxogram. Alternatively to the initial plan using the light switch, IPTG will be used as input to induce the initial production of GFP.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We intend to assembly all biobricks using the 3A assembly method, except the smaller parts, like RBS and terminal sequences in which will be used the Standard Assembly Method. Depending on the method of assembly, a different scheme of digestion will be used. The different types of digestion are on the assembly fluxogram. Alternatively to the initial plan using the light switch, IPTG will be used as input to induce the initial production of GFP.</div></td></tr>
</table>Pepekshttp://2012.igem.org/wiki/index.php?title=Team:USP-UNESP-Brazil/Associative_Memory/Experiments&diff=150379&oldid=prevPepeks: /* Assembly diagrams */2012-09-23T19:58:20Z<p><span class="autocomment">Assembly diagrams</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Previously, it was intent to use a light receptor (Red Light Suit) as input system. The strategy was to put the light responsive promoter in sequence to the Prm promoter, making the genic regulation of the following ORF. In order to simplify the Project, this part was removed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Previously, it was intent to use a light receptor (Red Light Suit) as input system. The strategy was to put the light responsive promoter in sequence to the Prm promoter, making the genic regulation of the following ORF. In order to simplify the Project, this part was removed.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>To illustrate all constructions used in all experiments, the Venn diagram ([https://static.igem.org/mediawiki/2012/2/2a/Assembly_Map.png<del class="diffchange diffchange-inline">/ </del>HERE]) was created, gathering all partial constructions in the main construction. In this LINK, there is the assembly fluxogram.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>To illustrate all constructions used in all experiments, the Venn diagram ([https://static.igem.org/mediawiki/2012/2/2a/Assembly_Map.png HERE]) was created, gathering all partial constructions in the main construction. In this LINK, there is the assembly fluxogram.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We intend to assembly all biobricks using the 3A assembly method, except the smaller parts, like RBS and terminal sequences in which will be used the Standard Assembly Method. Depending on the method of assembly, a different scheme of digestion will be used. The different types of digestion are on the assembly fluxogram. Alternatively to the initial plan using the light switch, IPTG will be used as input to induce the initial production of GFP.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We intend to assembly all biobricks using the 3A assembly method, except the smaller parts, like RBS and terminal sequences in which will be used the Standard Assembly Method. Depending on the method of assembly, a different scheme of digestion will be used. The different types of digestion are on the assembly fluxogram. Alternatively to the initial plan using the light switch, IPTG will be used as input to induce the initial production of GFP.</div></td></tr>
</table>Pepekshttp://2012.igem.org/wiki/index.php?title=Team:USP-UNESP-Brazil/Associative_Memory/Experiments&diff=150375&oldid=prevPepeks: /* Assembly diagrams */2012-09-23T19:57:56Z<p><span class="autocomment">Assembly diagrams</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 19:57, 23 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Previously, it was intent to use a light receptor (Red Light Suit) as input system. The strategy was to put the light responsive promoter in sequence to the Prm promoter, making the genic regulation of the following ORF. In order to simplify the Project, this part was removed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Previously, it was intent to use a light receptor (Red Light Suit) as input system. The strategy was to put the light responsive promoter in sequence to the Prm promoter, making the genic regulation of the following ORF. In order to simplify the Project, this part was removed.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>To illustrate all constructions used in all experiments, the Venn diagram (HERE<del class="diffchange diffchange-inline">(link)</del>) was created, gathering all partial constructions in the main construction. In this LINK, there is the assembly fluxogram.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>To illustrate all constructions used in all experiments, the Venn diagram (<ins class="diffchange diffchange-inline">[https://static.igem.org/mediawiki/2012/2/2a/Assembly_Map.png/ </ins>HERE<ins class="diffchange diffchange-inline">]</ins>) was created, gathering all partial constructions in the main construction. In this LINK, there is the assembly fluxogram.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We intend to assembly all biobricks using the 3A assembly method, except the smaller parts, like RBS and terminal sequences in which will be used the Standard Assembly Method. Depending on the method of assembly, a different scheme of digestion will be used. The different types of digestion are on the assembly fluxogram. Alternatively to the initial plan using the light switch, IPTG will be used as input to induce the initial production of GFP.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We intend to assembly all biobricks using the 3A assembly method, except the smaller parts, like RBS and terminal sequences in which will be used the Standard Assembly Method. Depending on the method of assembly, a different scheme of digestion will be used. The different types of digestion are on the assembly fluxogram. Alternatively to the initial plan using the light switch, IPTG will be used as input to induce the initial production of GFP.</div></td></tr>
</table>Pepekshttp://2012.igem.org/wiki/index.php?title=Team:USP-UNESP-Brazil/Associative_Memory/Experiments&diff=143478&oldid=prevPepeks: /* Test of Repression by cl434 */2012-09-22T21:32:05Z<p><span class="autocomment">Test of Repression by cl434</span></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 21:32, 22 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Test of Repression by cl434=== </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>===Test of Repression by cl434=== </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The lineage 2 of E.coli Will be created and induced, by means of IPTG, to produce cl434 and, consequently, inhibit the production of tetR repressor, which represses PtetR promoter, the controller of GFP production. By this way, the transcription of GFP by PtetR will be stimulated. By this way, like in the previous experiment, it is expected to observe the emerging of fluorescence. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The lineage 2 of E.coli Will be created and induced, by means of IPTG, to produce cl434 and, consequently, inhibit the production of tetR repressor, which represses PtetR promoter, the controller of GFP production. By this way, the transcription of GFP by PtetR will be stimulated. By this way, like in the previous experiment, it is expected to observe the emerging of fluorescence. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There is a possibility that the basal levels of Prm transcription be too high or low to maintain a proper feedback in the QS system. In an ideal situation, the Prm would have transcription rates similar to the QS system promoters (PcinRandPrlhR), emulating the natural feedback of the QS systems- not enough to activate the system. A way to do it would be create mutant Prm promoters with a set of different transcription rates. This would be a good alternative, but probably will not make part in this Project due the deadlines and time required to do it. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>There is a possibility that the basal levels of Prm transcription be too high or low to maintain a proper feedback in the QS system. In an ideal situation, the Prm would have transcription rates similar to the QS system promoters (PcinRandPrlhR), emulating the natural feedback of the QS systems- not enough to activate the system. A way to do it would be create mutant Prm promoters with a set of different transcription rates. This would be a good alternative, but probably will not make part in this Project due the deadlines and time required to do it. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Assembly diagrams</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">===</ins>Assembly diagrams<ins class="diffchange diffchange-inline">===</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Previously, it was intent to use a light receptor (Red Light Suit) as input system. The strategy was to put the light responsive promoter in sequence to the Prm promoter, making the genic regulation of the following ORF. In order to simplify the Project, this part was removed.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Previously, it was intent to use a light receptor (Red Light Suit) as input system. The strategy was to put the light responsive promoter in sequence to the Prm promoter, making the genic regulation of the following ORF. In order to simplify the Project, this part was removed.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To illustrate all constructions used in all experiments, the Venn diagram (HERE(link)) was created, gathering all partial constructions in the main construction. In this LINK, there is the assembly fluxogram.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To illustrate all constructions used in all experiments, the Venn diagram (HERE(link)) was created, gathering all partial constructions in the main construction. In this LINK, there is the assembly fluxogram.</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We intend to assembly all biobricks using the 3A assembly method, except the smaller parts, like RBS and terminal sequences in which will be used the Standard Assembly Method. Depending on the method of assembly, a different scheme of digestion will be used. The different types of digestion are on the assembly fluxogram. Alternatively to the initial plan using the light switch, IPTG will be used as input to induce the initial production of GFP.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We intend to assembly all biobricks using the 3A assembly method, except the smaller parts, like RBS and terminal sequences in which will be used the Standard Assembly Method. Depending on the method of assembly, a different scheme of digestion will be used. The different types of digestion are on the assembly fluxogram. Alternatively to the initial plan using the light switch, IPTG will be used as input to induce the initial production of GFP.</div></td></tr>
</table>Pepeks