Team:USP-UNESP-Brazil/Associative Memory/Experiments

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(Assembly diagrams)
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In order to construct the genic design (shown on the introductory part), we Will use bio bricks listed on appendix 1.
In order to construct the genic design (shown on the introductory part), we Will use bio bricks listed on appendix 1.
At first, before doing assemblies, we intend to confirm all parts that will be used. We will verify the lengths of the inserts in the plasmids using agarose gel and digestion by enzymes that cut in the prefixes and suffixes of BioBricks(EcoRI and PstI).
At first, before doing assemblies, we intend to confirm all parts that will be used. We will verify the lengths of the inserts in the plasmids using agarose gel and digestion by enzymes that cut in the prefixes and suffixes of BioBricks(EcoRI and PstI).
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We intend to assembly all biobricks using the 3A assembly method, except the smaller parts, like RBS and terminal sequences in which will be used the Standard Assembly Method. Depending on the method of assembly, a different scheme of digestion will be used. The different types of digestion are on the assembly fluxogram.  Alternatively to the initial plan using the light switch, IPTG will be used as input to induce the initial production of GFP.
We intend to assembly all biobricks using the 3A assembly method, except the smaller parts, like RBS and terminal sequences in which will be used the Standard Assembly Method. Depending on the method of assembly, a different scheme of digestion will be used. The different types of digestion are on the assembly fluxogram.  Alternatively to the initial plan using the light switch, IPTG will be used as input to induce the initial production of GFP.
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Revision as of 01:30, 26 September 2012