Team:USP-UNESP-Brazil/Associative Memory/Experiments

From 2012.igem.org

(Difference between revisions)
(Test of Repression by cl434)
(Assembly diagrams)
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Previously, it was intent to use a light receptor (Red Light Suit) as input system. The strategy was to put the light responsive promoter in sequence to the Prm promoter, making the genic regulation of the following ORF. In order to simplify the Project, this part was removed.
Previously, it was intent to use a light receptor (Red Light Suit) as input system. The strategy was to put the light responsive promoter in sequence to the Prm promoter, making the genic regulation of the following ORF. In order to simplify the Project, this part was removed.
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To illustrate all constructions used in all experiments, the Venn diagram (HERE(link)) was created, gathering all partial constructions in the main construction. In this LINK, there is the assembly fluxogram.
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To illustrate all constructions used in all experiments, the Venn diagram ([https://static.igem.org/mediawiki/2012/2/2a/Assembly_Map.png/ HERE]) was created, gathering all partial constructions in the main construction. In this LINK, there is the assembly fluxogram.
We intend to assembly all biobricks using the 3A assembly method, except the smaller parts, like RBS and terminal sequences in which will be used the Standard Assembly Method. Depending on the method of assembly, a different scheme of digestion will be used. The different types of digestion are on the assembly fluxogram.  Alternatively to the initial plan using the light switch, IPTG will be used as input to induce the initial production of GFP.
We intend to assembly all biobricks using the 3A assembly method, except the smaller parts, like RBS and terminal sequences in which will be used the Standard Assembly Method. Depending on the method of assembly, a different scheme of digestion will be used. The different types of digestion are on the assembly fluxogram.  Alternatively to the initial plan using the light switch, IPTG will be used as input to induce the initial production of GFP.

Revision as of 19:57, 23 September 2012