Team:USP-UNESP-Brazil/Associative Memory/Background

From 2012.igem.org

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In this case each position of the letter “X” inhibits all positions of “O” and activates the positions of its own pattern (and vice-versa).
In this case each position of the letter “X” inhibits all positions of “O” and activates the positions of its own pattern (and vice-versa).
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<!--In the Registry of Parts there are 4 quorum sensing systems well characterized, and there is a strong activation crosstalk between two of them (Las and Rhl), this fact prevent us from using them. Therefore, we end up with 3 systems of quorum sensing that can be used.-->
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{{:Team:USP-UNESP-Brazil/Templates/RImage | image=0019.JPG | caption=Fig. 3 | size=600px}}
{{:Team:USP-UNESP-Brazil/Templates/RImage | image=0019.JPG | caption=Fig. 3 | size=600px}}
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We decided to use two of the four quorum sensing systems available in the registry of parts [http://partsregistry.org/Main_Page], the Cin and Rhl.
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In the Registry of Parts [http://partsregistry.org/Main_Page] there are 4 quorum sensing systems well characterized. However, there is a strong activation crosstalk between two of them (Las and Rhl). Therefore, we decided to use the system Cin and Rhl.
To convert the signals in activation or inhibition, we created a system of transduction of the quorum sensing signal to transcription of an activator or an inhibitor of the transcription of GFP, this will be our system activation reporter. Simultaneous inhibitions and activations of a bacterial population will be converted to a molecular competition of activators and inhibitors by the promoter that controls the production of GFP. It is this molecular competition that promotes the decision between the memories of the communication systems, associating a given input with a more similar memory. As an example, if an input activates more positions of the “X” pattern than the “O”, the competition in the pattern “X” positions will be more favorable to its activation due to the greater number of activators produced by the activated positions, while in the positions of the “O” pattern the opposite occurs, because of its small number of positions activated initially by the given input.
To convert the signals in activation or inhibition, we created a system of transduction of the quorum sensing signal to transcription of an activator or an inhibitor of the transcription of GFP, this will be our system activation reporter. Simultaneous inhibitions and activations of a bacterial population will be converted to a molecular competition of activators and inhibitors by the promoter that controls the production of GFP. It is this molecular competition that promotes the decision between the memories of the communication systems, associating a given input with a more similar memory. As an example, if an input activates more positions of the “X” pattern than the “O”, the competition in the pattern “X” positions will be more favorable to its activation due to the greater number of activators produced by the activated positions, while in the positions of the “O” pattern the opposite occurs, because of its small number of positions activated initially by the given input.

Revision as of 18:27, 26 September 2012