Team:UC Davis/Safety

From 2012.igem.org

(Difference between revisions)
Line 80: Line 80:
         <link rel="stylesheet" type="text/css" href="https://2012.igem.org/forum/forum_styles.css" />
         <link rel="stylesheet" type="text/css" href="https://2012.igem.org/forum/forum_styles.css" />
<script type="text/javascript"        src ="https://2012.igem.org/forum/forum_scripts.js"></script>
<script type="text/javascript"        src ="https://2012.igem.org/forum/forum_scripts.js"></script>
 +
 +
<!-- slide script starts here -->
 +
<script type="text/javascript">
<script type="text/javascript">
-
$(document).ready(function(){
+
function slider() {
-
        $('.ntabs a').click(function() {
+
    var $current = $('#slides IMG.current');
-
$this = $(this);
+
 
-
$('.npanel').hide();
+
    if ( $current.length == 0 ) $current = $('#slides IMG:last');
-
$('.ntabs a.active').removeClass('active');
+
    var $next =  $current.next().length ? $current.next()
-
$this.addClass('active').blur();
+
        : $('#slides IMG:first');
-
var contents = $this.attr('href');
+
 
-
$(contents).fadeIn(250);  
+
      $current.addClass('previous');
-
return false;      
+
    $next.css({opacity: 0.0})
-
});
+
        .addClass('current')
-
$('.ntabs li:first a').click();
+
        .animate({opacity: 1.0}, 2500, function() {
 +
            $current.removeClass('current previous');
 +
        });
 +
 
 +
var $navicurrent = $('.progress li.current');
 +
if($navicurrent.length ==0) $navicurrent = $('.progress li:last');
 +
var $navinext = $navicurrent.next().length ? $navicurrent.next() : $('.progress li:first');
 +
$navicurrent.addClass('previous');
 +
$navicurrent.removeClass('current previous');
 +
$navinext.addClass('current').animate({opacity: 1.0}, 2500, function() {
 +
        });
 +
 
 +
$('.progress li').click(function(){
 +
   
 +
var $ncurrent = $(this);
 +
        $('.progress li').removeClass('current').removeClass('previous');
 +
        $('#slides IMG').removeClass('current').removeClass('previous');  
 +
        $ncurrent.addClass('current');
 +
        var ncurrentindex = $ncurrent.index();
 +
if(  ncurrentindex=== 0) $current = $('#slides IMG:first');
 +
        if(  ncurrentindex=== 1) $current = $('#slides IMG:eq(1)');
 +
        if(  ncurrentindex=== 2) $current = $('#slides IMG:eq(2)');
 +
        if(  ncurrentindex=== 3) $current = $('#slides IMG:last');
 +
        $current.addClass('current');
 +
 
 +
});
 +
 
 +
}
 +
$(function() {
 +
    setInterval( "slider()", 5000 );
});
});
$(document).ready(function(){
$(document).ready(function(){
-
$("#firstpane p.menu_head").click(function()
+
 
-
{
+
  $('a.lightbox').click(function(e) {
-
     $(this).css({backgroundImage:"url(./arrow_down.png)"}).next("div.menu_body").slideToggle(300).siblings("div.menu_body").slideUp("slow");
+
     $('body').css('display', 'inherit'); // hide scrollbars!
-
     $(this).siblings().css({backgroundImage:"url(./arrow_right.png)"});
+
 
-
});
+
    $('<div id="overlay"></div>')
 +
    .css('opacity', '0')
 +
      .animate({'opacity': '0.7'}, 'slow')
 +
    .click(function(){
 +
      removeLightbox();
 +
      })
 +
      .appendTo('body');
 +
 
 +
    $('<div id="lightbox"></div>')
 +
      .hide()
 +
      .appendTo('body');
 +
 
 +
     $('<img>')
 +
      .attr('src', $(this).attr('href'))
 +
      .load(function() {
 +
        positionLightboxImage();
 +
      })
 +
      .click(function() {
 +
        removeLightbox();
 +
      })
 +
      .appendTo('#lightbox');
 +
 
 +
    return false;
 +
  });
});
});
-
</script>
+
function positionLightboxImage() {
 +
  var top = ($(window).height()  - $('#lightbox').height())/2;
 +
  var left = ($(window).width() - $('#lightbox').width())/2;
 +
 
 +
  $('#lightbox')
 +
    .css({
 +
      'top': top,
 +
      'left':left-80
 +
    })
 +
    .fadeIn();
 +
}
 +
 
 +
function removeLightbox() {
 +
 +
  $('#overlay, #lightbox')
 +
    .fadeOut('slow', function() {
 +
      $(this).remove();
 +
      $('body').css('overflow-y', 'auto'); // show scrollbars!
 +
    });
 +
}
 +
 
 +
 
 +
</script>
 +
<!-- slide script ends here -->
<style type="text/css">
<style type="text/css">
<!-- beginning of slider style -->
<!-- beginning of slider style -->
Line 110: Line 188:
min-height:100%; /* real browsers */
min-height:100%; /* real browsers */
}
}
-
#content { z-index: 1; background-color: transparent; border: none; padding: 0; margin: 0; width: 100%; overflow: hidden; margin-top: -45px !important;
+
#content { z-index: 1; background-color: transparent; border: none; padding: 0; margin: 0; width: 100%; overflow: hidden; top:-3px; margin-top: -45px !important;
height:auto !important; /* real browsers */
height:auto !important; /* real browsers */
height:100%; /* IE6: treaded as min-height*/
height:100%; /* IE6: treaded as min-height*/
Line 127: Line 205:
#menubar.right-menu { width:300px; display:block; float:left; margin-top:-80px; border: none;}
#menubar.right-menu { width:300px; display:block; float:left; margin-top:-80px; border: none;}
.right-menu ul { border: none; width: 300px;}
.right-menu ul { border: none; width: 300px;}
-
#footer-box { background-color: #216085; border: none; width: 100%; height: 100%; margin: -10px auto 0 auto; padding: 20px 0;}
+
#footer-box { background-color: #216085; border: none; width: 850px; height: 30px; margin: -10px auto 0 auto; padding: 20px 0;}
.visualClear { display: none; }
.visualClear { display: none; }
#footer { border: none; width: 850px; margin: 0 auto; padding: 0;}
#footer { border: none; width: 850px; margin: 0 auto; padding: 0;}
Line 139: Line 217:
h3#siteSub { display: none;}
h3#siteSub { display: none;}
#contentSub {display: none;}
#contentSub {display: none;}
-
p:first-child { display: none;}
+
p:first-child { display: block;}
h1{border:none; width: 100%; clear: both;}
h1{border:none; width: 100%; clear: both;}
-
<!-- end of slides style -->
 
#globalWrapper {
#globalWrapper {
Line 238: Line 315:
         position:relative;
         position:relative;
         height:42px;
         height:42px;
-
         width: 856px;
+
         width: 850px;
         margin-left: -2px;
         margin-left: -2px;
         display:block;
         display:block;
Line 250: Line 327:
         list-style-type:none;  
         list-style-type:none;  
         height:24px;
         height:24px;
 +
        width: 107px;
         list-style-image:none;
         list-style-image:none;
         background: #004b85;
         background: #004b85;
Line 255: Line 333:
#newnavi .newmenu li    {
#newnavi .newmenu li    {
         float:right;  
         float:right;  
-
        height:24px;
 
-
        width: 103px;
 
         background: #004b85;
         background: #004b85;
-
#        margin-left: solid 2px transparent;
+
        text-align:center;
-
#        margin-right: solid 2px transparent;
+
-
#        margin-bottom: solid 1px transparent;
+
-
#        border-right: 4px solid #eaf0f2;
+
-
#        border-left: 2px solid #eaf0f2;
+
-
        border-right: 2px solid transparent;
+
-
        border-left: 2px solid transparent;
+
-
        text-align:center;
+
}
}
#newnavi .newmenu .selected {
#newnavi .newmenu .selected {
Line 271: Line 340:
}
}
#newnavi .newmenu li a  {
#newnavi .newmenu li a  {
-
text-align:center; vertical-align:middle; width:82px; padding:5px 10px 5px 10px; display:block; color:#fff; text-decoration:none; font:14px arial, verdana, sans-serif; font-weight: bold;
+
text-align:center; vertical-align:middle; width:86px; padding:5px 10px 5px 10px; display:block; color:#fff; text-decoration:none; font:14px arial, verdana, sans-serif; font-weight: bold;
}
}
#newnavi .newmenu li:hover a {
#newnavi .newmenu li:hover a {
Line 277: Line 346:
}
}
#newnavi .newmenu ul    {
#newnavi .newmenu ul    {
-
position:absolute; left:-9999px; top:-9999px; list-style-type:none; list-style-image:none; margin:0px; border:0px; padding:0px; background:lavender;
+
position:absolute; left:-9999px; top:-9999px; list-style-type:none; list-style-image:none; margin:0px; border:0px; padding:0px; background: #05bcea;
}
}
#newnavi .newmenu li:hover      {
#newnavi .newmenu li:hover      {
Line 283: Line 352:
}
}
#newnavi .newmenu li:hover ul  {
#newnavi .newmenu li:hover ul  {
-
left:0px; top:23px; background:lavender;  width:103px; opacity:0.7; z-index:4;
+
left:0px; top:26px; background: #05bcea;  width:150px; opacity:1.0 ; z-index:4;
}
}
#newnavi .newmenu li:hover ul li        {
#newnavi .newmenu li:hover ul li        {
-
         height:17px;
+
          
-
        border:none;
+
         background: #05bcea;
-
         background:lavender;
+
}
}
#newnavi .newmenu li:hover ul li a      {
#newnavi .newmenu li:hover ul li a      {
-
text-align:center; height:17px; padding:0px;display:block; font-size:12px; width:103px; line-height:16px; text-indent:0px; color:#444;  
+
text-align:left; padding:5px 0px 5px 0px;display:block; font-size:12px; width:150px; text-indent:10px; color:#444;  
-
background-color:transparent; text-decoration:none; border:0px solid transparent;       
+
background-color: #05bcea; text-decoration:none;       
}
}
#newnavi .newmenu li:hover ul li a:hover {
#newnavi .newmenu li:hover ul li a:hover {
-
height:18px; background:silver; color:#000; border:solid 0px #444;       
+
background: #05bcea; color:#ffffff; width:150px;       
}
}
-
/* slide starts here */
+
 
#slides {
#slides {
     position:relative;
     position:relative;
-
     height:350px;
+
     height:280px;
     width: 840px
     width: 840px
      
      
Line 321: Line 389:
     z-index:2;
     z-index:2;
}
}
-
/* slide ends here */
 
-
//leftbox, rightbox starts here
 
 +
.progress {
 +
 +
margin-top:-270px;
 +
margin-left:20px;
 +
position:absolute;
 +
 +
}
 +
 +
.progress li{
 +
border:1px solid rgba(0,0,0,0.9);
 +
border-radius:3px;
 +
margin-left: 5px;
 +
float:left;
 +
display:block;
 +
position:relative;
 +
z-index:10;
 +
background-color:rgba(255,255,255,0.4);
 +
}
 +
 +
.progress li a{
 +
    padding-left: 6px;
 +
    padding-right: 6px;
 +
}
 +
 +
.progress li a:hover {
 +
text-decoration:none;
 +
}
 +
 +
.progress .current {
 +
background-color:white;
 +
}
 +
 +
#myleftrightbox
 +
{
 +
position:relative;
 +
width:850px;
 +
float:left;
 +
background-color:transparent;
 +
margin-top: 0px;
 +
margin-left:3.75px solid transparent;
 +
margin-right:3.75px solid transparent;
 +
border-radius: 4px;
 +
padding: 0px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftrightbox .fourboxes
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftrightbox .fourboxes-1
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftrightbox .fourboxes-2
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftrightbox .fourboxes-3
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftrightbox .fourboxes-4
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftrightbox .fourboxes-1:hover {
 +
background-color:green;
 +
}
 +
#myleftrightbox .fourboxes-2:hover {
 +
background-color:orange;
 +
}
 +
#myleftrightbox .fourboxes-3:hover {
 +
background-color:#05bcea;
 +
}
 +
#myleftrightbox .fourboxes-4:hover {
 +
background-color:#676767;
 +
}
 +
 +
 +
#myleftrightbox .fourboxes2
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 5px;
 +
margin-left:3.75px solid #e8eff1;
 +
margin-right:3.75px solid #e8eff1;
 +
margin-bottom:0px;
 +
border-radius: 4px;
 +
padding: 8.5px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftrightbox .spacebox
 +
{
 +
float:left;
 +
width:5px;
 +
 +
}
#myleftbox
#myleftbox
{
{
Line 329: Line 559:
width:625px;
width:625px;
float:left;
float:left;
-
background-color:#d2d2d2;
+
background-color:;
margin-top: 0px;
margin-top: 0px;
border-radius: 4px;
border-radius: 4px;
Line 339: Line 569:
#myleftbox .smallbox  
#myleftbox .smallbox  
{
{
-
width:595px;
+
width:604px;
float:left;
float:left;
background-color:#bfbfbf;
background-color:#bfbfbf;
-
margin-top: 17px;
+
margin-top: 15px;
-
margin-bottom: 10px;
+
border-radius: 4px;
border-radius: 4px;
padding: 15px 15px 15px 15px;
padding: 15px 15px 15px 15px;
Line 361: Line 590:
font-weight:bold;
font-weight:bold;
}
}
 +
#myleftbox .smallboxsite
 +
{
 +
width:604px;
 +
float:left;
 +
background-color:rgba(191,191,191,0.6 );
 +
margin-top: 17px;
 +
border-radius: 4px;
 +
padding: 15px 15px 15px 15px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
line-height:1.5em;
 +
color:black;
 +
}
#myleftbox .threeboxes
#myleftbox .threeboxes
{
{
-
width:175px;
+
width:191px;
float:left;
float:left;
background-color:rgba(143,143,143,0.7);
background-color:rgba(143,143,143,0.7);
-
margin-top: 17px;
+
margin-top: 15px;
border-radius: 4px;
border-radius: 4px;
border-left:2px solid #e8eff1;
border-left:2px solid #e8eff1;
border-right:2px solid #e8eff1;
border-right:2px solid #e8eff1;
-
padding: 15px 15px 15px 15px;
+
padding: 7px 7px 7px 7px;
font: sans-serif;
font: sans-serif;
font-size: 13px;
font-size: 13px;
color: white;
color: white;
 +
}
 +
#myleftbox .threeboxes2
 +
{
 +
width:191px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 5px;
 +
border-radius: 4px;
 +
border-left:2px solid #e8eff1;
 +
border-right:2px solid #e8eff1;
 +
padding: 7px 7px 7px 7px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
}
}
Line 383: Line 639:
float:left;
float:left;
background-color:#ba9108;
background-color:#ba9108;
-
margin-top: 17px;
+
margin-top: -52px;
border-radius: 4px;
border-radius: 4px;
padding: 15px 15px 15px 15px;
padding: 15px 15px 15px 15px;
Line 396: Line 652:
#float:left;
#float:left;
background-color:#ba9108;
background-color:#ba9108;
-
margin-top: 17px;
+
margin-top: -52px;
border-radius: 4px;
border-radius: 4px;
padding: 15px 15px 15px 15px;
padding: 15px 15px 15px 15px;
Line 408: Line 664:
float:right;
float:right;
background-color:#ba9108;
background-color:#ba9108;
-
margin-top: 17px;
+
margin-top: -52px;
border-radius: 4px;
border-radius: 4px;
padding: 15px 15px 15px 15px;
padding: 15px 15px 15px 15px;
Line 414: Line 670:
font-size: 13px;
font-size: 13px;
color: white;
color: white;
 +
}
 +
 +
 +
 +
#myleftbox .twoboxes
 +
{
 +
width:295px;
 +
height:120px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
border-radius: 4px;
 +
border-left:2px solid #e8eff1;
 +
border-right:2px solid #e8eff1;
 +
padding: 7px 7px 7px 7px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
}
 +
 +
#myleftbox .twoboxes1
 +
{
 +
width:295px;
 +
height:120px;
 +
float:left;
 +
background-color:rgba(143,143,143,0.7);
 +
margin-top: 15px;
 +
border-radius: 4px;
 +
border-left:1px solid #e8eff1;
 +
border-right:2px solid #e8eff1;
 +
padding: 7px 7px 7px 7px;
 +
font: sans-serif;
 +
font-size: 13px;
 +
color: white;
 +
left:9px;
 +
}
 +
 +
#myleftbox .twoboxes:hover {
 +
background-color:red;
 +
}
 +
 +
#myleftbox .twoboxes1:hover {
 +
background-color:green;
}
}
Line 419: Line 718:
{
{
position: relative;
position: relative;
-
width: 215px;
+
width: 208px;
-
margin-top:17px;
+
margin-top:15px;
float: right;
float: right;
height: auto;
height: auto;
Line 427: Line 726:
#sponsorbox .sponsorfloat
#sponsorbox .sponsorfloat
{
{
-
width:205px;
+
left:-3px;
 +
width:198px;
float: right;
float: right;
background-color: #004b85;
background-color: #004b85;
Line 445: Line 745:
font-size:130%;
font-size:130%;
}
}
-
//leftbox, rightbox ends here
 
#sponsorbox .sponsorfloat a
#sponsorbox .sponsorfloat a
{
{
color:white;
color:white;
 +
font: sans-serif;
 +
}
 +
 +
#sponsorbox .sponsorfloat p
 +
{
 +
font: sans-serif;
}
}
.newsAnnouncement
.newsAnnouncement
Line 458: Line 763:
overflow-y:scroll;
overflow-y:scroll;
}
}
-
<!-- notebook tab style starts here -->
 
-
.ntabs {
 
-
margin: 0;
 
-
padding: 0;
 
-
zoom : 1;
 
-
}
 
-
.ntabs li {
 
-
float: left;
 
-
list-style: none;
 
-
padding: 0;
 
-
margin: 0;
 
-
}
 
-
.ntabs a {
 
-
display: block;
 
-
text-decoration: none;
 
-
padding: 1px 8px 1px 8px;
 
-
background-color: #ba9108;
 
-
margin-right:1px;
 
-
border: 1px solid rgb(153,153,153);
 
-
        border-radius: 4px 4px 0px 0px;
 
-
margin-bottom: -1px;
 
-
font-size:14px;
 
-
line-height:25px;
 
-
}
 
-
.ntabs a.active {
 
-
border-bottom: 1px solid #fdf2b2;
 
-
background-color: #fdf2b2;
 
-
color: #004b85;
 
-
position: relative;
 
-
}
 
-
.ntabs a:hover {
 
-
        background-color: rgba(186,145,8,0.3);     
 
-
}
 
-
.npanelContainer {
 
-
background:url("./notepad_pattern.jpg") repeat;
 
-
clear: both;
 
-
margin-bottom: 25px;
 
-
border: 1px solid rgb(153,153,153);
 
-
background-color: white;
 
-
padding: 17px 0 0 10px;;
 
-
        font-size:14px;
 
-
        line-height:25px;
 
-
        border-radius:4px;
 
-
}
 
-
 
-
.npanel h2 {
 
-
color: rgb(57,78,121);
 
-
text-shadow: none;
 
-
}
 
-
.npanel p {
 
-
color: black;
 
-
}
 
-
<!-- notebook tab style ends here -->
 
-
#firstpane {
 
-
position:relative;
 
-
}
 
-
#firstpane p {
 
-
display:block;
 
-
}
 
-
#firstpane .menu_head {
 
-
padding: 5px 20px;
 
-
cursor: pointer;
 
-
position: relative;
 
-
margin:1px;
 
-
      font-weight:bold;
 
-
      background: #eef4d3 url(./arrow_right.png) center left no-repeat;
 
-
background-position: 5px center;
 
-
        background-size: 0.7em auto;
 
-
}
 
-
 
-
#firstpane .menu_body {
 
-
display:none;
 
-
background-color:#e1e1e1;
 
-
        background-image:none !important;
 
-
        margin-top:-5px
 
-
        border-radius:4px;
 
-
        padding:10px;
 
-
}
 
-
#firstpane .menu_body a {
 
-
  display:block;
 
-
  color:#006699;
 
-
  background-color:#EFEFEF;
 
-
  padding-left:10px;
 
-
  font-weight:bold;
 
-
  text-decoration:none;
 
-
}
 
-
#firstpane .menu_body a:hover {
 
-
  color: #000000;
 
-
  text-decoration:underline;
 
-
}
 
-
 
.floatbox3
.floatbox3
Line 596: Line 810:
     padding: 0 .0em;}
     padding: 0 .0em;}
-
 
+
#catlinks {
 +
background-color:transparent;
 +
border:1px solid transparent;
 +
}
 +
#footpage {
 +
background-color:transparent;
 +
}
 +
#footer-box {
 +
background-color:rgba(191,191,191,0.5);
 +
margin-top:0px;
 +
}
#footer-box-1 {
#footer-box-1 {
          
          
Line 605: Line 829:
         border: 1px solid #444444;
         border: 1px solid #444444;
}
}
 +
#tweets {
 +
    width: 203px;
 +
    margin: 0 auto;
 +
    font: sans-serif;
 +
    font-size: 12px;
 +
}
 +
 +
#tweets .twtr-widget,
 +
#tweets .twtr-doc {
 +
 +
    width: 100%;
 +
    height: auto;
 +
}
 +
 +
#tweets .twtr-hd {
 +
    display:none;
 +
    background: #d40;
 +
    color: #fff;
 +
    -moz-border-radius: 4px 4px 0 0;
 +
    border-radius: 4px 4px 0 0;
 +
    font-family: sans-serif !important;
 +
    }
 +
 +
#tweets .twtr-hd *,
 +
#tweets .twtr-hd h4 a {
 +
 +
    background: #d40 !important;
 +
    font: sans-serif !important;
 +
}
 +
 +
#tweets .twtr-hd h3,
 +
#tweets .twtr-hd h4 {
 +
 +
    font-weight: normal;
 +
    text-align: left;
 +
    margin:0;
 +
}
 +
 +
#tweets .twtr-hd h3 {
 +
       
 +
    background: #fff !important;
 +
    color: #333 !important;
 +
    font: sans-serif;
 +
    font-size: 1.0em !important;
 +
    padding: 0.2em !important;
 +
    -moz-border-radius: 0px;
 +
    border-radius: 0px;
 +
 +
}
 +
 +
#tweets .twtr-tweet {
 +
 +
    background: #fff;
 +
    font: sans-serif !important;
 +
    font-size: 12px;
 +
}
 +
 +
#tweets .twtr-tweet a:link,
 +
#tweets .twtr-tweet a:visited,
 +
#tweets .twtr-tweet a:hover {
 +
 +
    color: #c40 !important;
 +
 +
}
 +
 +
#tweets .twtr-tweet .twtr-tweet-text {
 +
 +
    border-bottom: 1px dashed #666 !important;
 +
    padding-bottom: 4px !important;
 +
    font: sans-serif;
 +
    font-size:12px;
 +
}
 +
 +
#tweets .twtr-tweet .twtr-tweet-text p {
 +
 +
    color: #333 !important;
 +
    font: sans-serif;
 +
    font-size: 12px;
 +
 +
}
 +
 +
#overlay {
 +
  z-index:2;
 +
  position:fixed;
 +
  top: 0;
 +
  left: 0;
 +
  height: 100%;
 +
  width: 100%;
 +
  background: black no-repeat scroll center center;
 +
}
 +
 +
#lightbox {
 +
  position: fixed;
 +
  width:840;
 +
  z-index:2;
 +
}
</style>
</style>
Line 644: Line 964:
         </ul>
         </ul>
         </li>
         </li>
-
         <li ><a href="https://2012.igem.org/Team:UC_Davis/Notebook" title="Notebook">Notebook</a>
+
         <li ><a href="https://2012.igem.org/Notebook" title="Notebook">Notebook</a>
           <ul>
           <ul>
 +
            <li ><a href="https://2012.igem.org/Team:UC_Davis/Notebook/Overview ">Overview</a></li>
             <li ><a href="https://2012.igem.org/Team:UC_Davis/Notebook/Protocols ">Protocols</a></li>
             <li ><a href="https://2012.igem.org/Team:UC_Davis/Notebook/Protocols ">Protocols</a></li>
-
             <li ><a href="Notebook.htm ">Week 1</a></li>
+
             <li ><a href="https://2012.igem.org/Team:UC_Davis/Notebook ">Notebook</a></li>
-
            <li ><a href="Notebook.htm ">Week 2</a></li>
+
-
            <li ><a href="Notebook.htm ">Week 3</a></li>
+
           </ul>
           </ul>
         </li>
         </li>
-
         <li class="selected"><a href="https://2012.igem.org/Team:UC_Davis/Safety" title="Safety">Safety</a></li>
+
         <li class="selected"><a href="https://2012.igem.org/Team:UC_Davis/Safety" title="Safety">Safety</a></li>
         <li ><a href="https://2012.igem.org/Team:UC_Davis/Project" title="Project">Project</a>
         <li ><a href="https://2012.igem.org/Team:UC_Davis/Project" title="Project">Project</a>
           <ul>
           <ul>
-
             <li ><a href="https://2012.igem.org/Team:UC_Davis/Project/Strain">Strain</a></li>
+
             <li ><a href="https://2012.igem.org/Team:UC_Davis/Project">Project Overview</a></li>
-
             <li ><a href="https://2012.igem.org/Team:UC_Davis/Project/Catalyst">Catalyst</a></li>
+
             <li ><a href="https://2012.igem.org/Team:UC_Davis/Project/Catalyst">Modular Engineering</a></li>
-
             <li ><a href="https://2012.igem.org/Team:UC_Davis/Project/Protein_Engineering" style="font-size:8pt">Protein Engineering</a></li>
+
             <li ><a href="https://2012.igem.org/Team:UC_Davis/Project/Strain">Chassis Engineering</a></li>
 +
            <li ><a href="https://2012.igem.org/Team:UC_Davis/Project/Protein_Engineering">Protein Engineering</a></li>
           </ul>
           </ul>
         </li>
         </li>
Line 666: Line 986:
     </ul>
     </ul>
   </div>
   </div>
-
<!--we extend the main-content div further by removing </div> or if you want to stop here then put </div> back here -->
+
<!-- slide show starts here
-
<img src="http://img.photobucket.com/albums/v26/bluemelon/safety_banner1.jpg" width="850" height="214">
+
<div id="slides">
 +
 
 +
    <img  src="http://img.photobucket.com/albums/v26/bluemelon/slide-1-2.jpg" width="850" height="280" alt="" class="current" />
 +
    <img  src="http://img.photobucket.com/albums/v26/bluemelon/slide-2-2.jpg" width="850" height="280" alt="" />
 +
    <img  src="http://img.photobucket.com/albums/v26/bluemelon/slide-3-2.jpg" width="850" height="280" alt="" />
 +
    <img src="http://img.photobucket.com/albums/v26/bluemelon/slide-4-2.jpg" width="850" height="280" alt="" />
 +
</div>
 +
    <ul class="progress">
 +
<li class="current"><a href="#n_0">1</a> </li>
 +
<li><a href="#n_1">2</a> </li>
 +
        <li><a href="#n_2">3</a> </li>
 +
        <li><a href="#n_3">4</a> </li>
 +
    </ul>
 +
slide show ends here -->
 +
 
 +
<img src="https://static.igem.org/mediawiki/2012/5/54/UCD_safety_banner.jpg">
 +
 
-
<div id="bodyContent"> 
 
-
<!--  <div id="contentSub"></div> -->
 
-
<!--  </div> -->
 
-
<!-- float: right; first try -->
 
<!-- <div id="sponsorbox" style="width:220px; float:right;"> -->
<!-- <div id="sponsorbox" style="width:220px; float:right;"> -->
<div id="sponsorbox">
<div id="sponsorbox">
<div id="sponsorbox" class="sponsorfloat">
<div id="sponsorbox" class="sponsorfloat">
 +
<!-- twitter starts here -->
 +
</script>
 +
<div id="tweets">
<center>
<center>
-
<h2>News</h2>
+
<h2>UCDavis iGEM Tweets</h2>
</center>
</center>
-
<div class="newsAnnouncement">
+
<script charset="utf-8" src="http://widgets.twimg.com/j/2/widget.js"></script>
-
<ul>
+
<script>
-
<li>Presented our respective project at the UC San Francisco campus. We interacted with teams from UC Berkeley, UCSF, and Stanford-Brown.  </li>
+
new TWTR.Widget({
-
<li>Requested the protocal from the iGEM Washington 2011 team, July 10th. </li>
+
  version: 2,
-
<li>Field trip to Yolo County Landfill, June 27th. Met with Ramin Yazdani, the senior civil engineer at the landfill. </li>
+
  type: 'profile',
-
<li>Sushi buffet for lunch, June 21st</li>
+
  rpp: 10,
-
<li>First day of iGEM meeting, June 18th</li>
+
  interval: 30000,
-
</ul>
+
  width: 203,
 +
  height: 100,
 +
 
 +
  theme: {
 +
    shell: {
 +
      background: '#004b85',
 +
      color: '#ffffff'
 +
    },
 +
    tweets: {
 +
      background: '#004b85',
 +
      color: '#ffffff',
 +
      links: '#4aed05'
 +
    }
 +
   
 +
  },
 +
  features: {
 +
    scrollbar: true,
 +
    loop: false,
 +
    live: true,
 +
    behavior: 'all'
 +
  }
 +
}).render().setUser('UCDavisiGEM').start();
 +
 
 +
</script>
</div>
</div>
-
</div>
+
<!-- twitter ends here -->
-
 
+
-
<div id="sponsorbox" class="sponsorfloat">
+
-
<center>
+
-
<h2>Criteria</h2>
+
-
View our judging criteria for iGEM 2012 <a href="https://2012.igem.org/Team:UC_Davis/Criteria" style="color:#33FF33;">here</a>.
+
-
</center>
+
</div>
</div>
Line 725: Line 1,077:
</div>
</div>
-
 
-
<!--
 
-
<a href="https://2011.igem.org/Team:UC_Davis/PartFamilies"><div id="sponsorbox" class="sponsorfloat"></a>
 
-
<center>
 
-
<h2>Start a Family</h2>
 
-
Got a favorite BioBrick? Check our our <a href="https://2011.igem.org/Team:UC_Davis/PartFamilies">process</a> for expanding basic parts into part families.
 
</div>
</div>
-
-->
+
<!-- <br> -->
 +
<!-- div id="myleftbox" style="width:625px; float:left;" -->
 +
<div id="myleftbox">
-
</div>
 
-
 
-
<div id="myleftbox">
 
<div id="myleftbox"  class="smallbox">
<div id="myleftbox"  class="smallbox">
-
<!-- Safety content starts here -->
+
<h1>Safety</h1>
-
 
+
-
<h1> Safety </h1>
+
<article>
<article>
-
<br>Our team made sure to perform a thorough analysis of safety for all parts of our project, since safety for researchers, the public, and the environment are all important. We work within a Level 2 Biosafety Lab, as defined by the <i>Laboratory Biosafety Manual</i> (1). This facility is well equipped to work with all the strains of E. coli we used. We used MG1655, DH5α, and K12, all of which are Risk Level 1, meaning these strains pose no pathogenic risk to humans, and were completely contained within the lab.  
+
Our team made sure to perform a thorough analysis of safety for all parts of our project, since safety for researchers, the public, and the environment are all important. We work within a Level 2 Biosafety Lab, as defined by the <i>Laboratory Biosafety Manual</i> (1). This facility is well equipped to work with all the strains of E. coli we used. We used MG1655, DH5α, and K12, all of which are Risk Level 1, meaning these strains pose no pathogenic risk to humans, and were completely contained within the lab.  
<br><br>
<br><br>
 +
<img src="http://img.photobucket.com/albums/v26/bluemelon/UCD_autoclave3.jpg" align="left">
We always made sure to make use of protective gear, such as wearing gloves at all times, masks when using UV lamps, and lab coats when appropriate. All of our waste was autoclaved on site as well, and all tools and glassware thoroughly cleaned after each use.   
We always made sure to make use of protective gear, such as wearing gloves at all times, masks when using UV lamps, and lab coats when appropriate. All of our waste was autoclaved on site as well, and all tools and glassware thoroughly cleaned after each use.   
<br><br>
<br><br>
Line 750: Line 1,094:
We worked with ethylene glycol during our experiments, which is toxic to the kidney, liver and central nervous system, as well as a skin and eye irritant. Obviously, we wanted to be very safe when using this chemical, so we made sure to consult with our advisers on how best to handle it. When using ethylene glycol, we always worked under a fume hood and wore lab coats.  We had a separate waste bottle for all of the byproducts with ethylene glycol, so that the waste could be treated.  
We worked with ethylene glycol during our experiments, which is toxic to the kidney, liver and central nervous system, as well as a skin and eye irritant. Obviously, we wanted to be very safe when using this chemical, so we made sure to consult with our advisers on how best to handle it. When using ethylene glycol, we always worked under a fume hood and wore lab coats.  We had a separate waste bottle for all of the byproducts with ethylene glycol, so that the waste could be treated.  
<br><br>
<br><br>
-
 
The University of California at Davis has a biosafety group, which heads the disposal and use of hazardous materials. They approve of our project and the procedures that we have followed. Further, they are excited for the potential that this project has, in terms of making the environment safer for inhabitants.
The University of California at Davis has a biosafety group, which heads the disposal and use of hazardous materials. They approve of our project and the procedures that we have followed. Further, they are excited for the potential that this project has, in terms of making the environment safer for inhabitants.
<br><br>
<br><br>
Line 756: Line 1,099:
Our BioBricked parts do not raise any safety concerns, as long as the enzymes breaking down ethylene glycol are fully functional.  
Our BioBricked parts do not raise any safety concerns, as long as the enzymes breaking down ethylene glycol are fully functional.  
<br><br>
<br><br>
 +
<img src="http://img.photobucket.com/albums/v26/bluemelon/UCD_sterile3.jpg" align="right">
Our project, when properly functioning, should not have any harmful effect on the environment, researchers, or the public. We keep everything contained within the lab, and all of the plates and wastes are autoclaved to prevent all bacteria we use from spreading to the outside environment. If we were to escalate our project to an industrial level, everything would still be contained within the facility.  
Our project, when properly functioning, should not have any harmful effect on the environment, researchers, or the public. We keep everything contained within the lab, and all of the plates and wastes are autoclaved to prevent all bacteria we use from spreading to the outside environment. If we were to escalate our project to an industrial level, everything would still be contained within the facility.  
Line 762: Line 1,106:
If we were to implement our bacteria in a landfill, chances are it could not survive past a couple generations and therefore would not be very toxic. However, if we were to imagine a more robust version of our strain created in the future, we would implement a kill switch, which has already been created using synthetic biology, to make sure we could stop it (2). We would also create a system similar to Imperial College London’s Gene Guard, which would stop to plastic-degrading plasmid from getting transferred to other bacteria (3). This would contain the plasmid within only our bacteria, so it would not get out of hand.  
If we were to implement our bacteria in a landfill, chances are it could not survive past a couple generations and therefore would not be very toxic. However, if we were to imagine a more robust version of our strain created in the future, we would implement a kill switch, which has already been created using synthetic biology, to make sure we could stop it (2). We would also create a system similar to Imperial College London’s Gene Guard, which would stop to plastic-degrading plasmid from getting transferred to other bacteria (3). This would contain the plasmid within only our bacteria, so it would not get out of hand.  
<br><br>
<br><br>
-
 
Our project could potentially be dangerous if our strain received a pathogenic plasmid by lateral gene transfer from other organisms. However, if this were a concern, our strain would be just a susceptible to pathogenicity as any other. If someone with malicious intent were to use our construct, perhaps the most they could do is release it into the water way and have it degrade plastic pipes made of PET, or modify it to degrade other plastics in addition to PET and degrade something useful. Cutinase, the first enzyme in our degradation pathway, functions optimally at 50° C, and the water in our pipes would most likely not be at that temperature. Also, it is unlikely the strain would survive under conditions such as this temperature and being in an aquatic environment.  
Our project could potentially be dangerous if our strain received a pathogenic plasmid by lateral gene transfer from other organisms. However, if this were a concern, our strain would be just a susceptible to pathogenicity as any other. If someone with malicious intent were to use our construct, perhaps the most they could do is release it into the water way and have it degrade plastic pipes made of PET, or modify it to degrade other plastics in addition to PET and degrade something useful. Cutinase, the first enzyme in our degradation pathway, functions optimally at 50° C, and the water in our pipes would most likely not be at that temperature. Also, it is unlikely the strain would survive under conditions such as this temperature and being in an aquatic environment.  
 +
<br><br>
 +
<img src="http://img.photobucket.com/albums/v26/bluemelon/UCD_UV3.jpg" align="left">
 +
 +
<img src="http://img.photobucket.com/albums/v26/bluemelon/UCD_fumehood3.jpg" align="center">
 +
 +
<img src="http://img.photobucket.com/albums/v26/bluemelon/UCD_gloves3.jpg" align="right">
 +
<br>
</article>
</article>
</div>
</div>
Line 769: Line 1,119:
<div id="myleftbox"  class="smallbox">
<div id="myleftbox"  class="smallbox">
<h1> Questions </h1>
<h1> Questions </h1>
-
 
<article>
<article>
<b>1. Would any of your project ideas raise safety issues in terms of: researcher safety, public safety, or environmental safety?</b>
<b>1. Would any of your project ideas raise safety issues in terms of: researcher safety, public safety, or environmental safety?</b>
Line 805: Line 1,154:
</div>
</div>
-
<div id="myleftbox"  class="smallbox">
+
 
 +
<br>
 +
<div id="myleftbox"  class="smallbox"><h1> References </h1>
<article>
<article>
-
<h1>References</h1>
 
1. <i>Laboratory Biosafety Manual</i>. The World Health Organization. 2004. 3rd edition.  
1. <i>Laboratory Biosafety Manual</i>. The World Health Organization. 2004. 3rd edition.  
<br>2. Callura, Jared, Dwyer, Daniel, Isaacs, Farren, Cantor, Charles, Collins, James. “Tracking, tuning, and terminating microbial physiology using synthetic riboregulators”. PNAS vol. 107 no. 36, pp. 15898-15903. Web. 13 July 2010.  
<br>2. Callura, Jared, Dwyer, Daniel, Isaacs, Farren, Cantor, Charles, Collins, James. “Tracking, tuning, and terminating microbial physiology using synthetic riboregulators”. PNAS vol. 107 no. 36, pp. 15898-15903. Web. 13 July 2010.  
<br>3. “Gene Overview”, <i>Imperial College London iGEM Wiki 2011</i>.
<br>3. “Gene Overview”, <i>Imperial College London iGEM Wiki 2011</i>.
-
<br><br>
 
-
 
</article>
</article>
</div>
</div>
 +
 +
 +
 +
<!-- site map starts here -->
 +
<div id="myleftbox"  class="smallboxsite">
 +
<ul style="font-size:10px;list-style-image:none;list-style-type:none;float:left;display:inline;color:#000000;"  >
 +
 +
<li style="float:left;margin:0 10px solid red"><a href="https://2012.igem.org/Team:UC_Davis"><p>Home</p><ul style="text-indent:-15px;
 +
list-style-image:none;list-style-type:none;color:#000000;"><li>Welcome </li><li>Tweets </li><li>Criteria </li><li>Sponsors </li> </ul> </a> </li>
 +
 +
<li style="float:left ;margin:0 10px;"><a href="https://2012.igem.org/Team:UC_Davis/Team"><p>Team</p> <ul style="text-indent:-15px;list-style-image:none;
 +
list-style-type:none;color:#000000 "><li>Who we are </li><li>Students </li><li>Advisors</li></ul></a> </li>
 +
 +
<li style="float:left ;margin:0 10px;"><a href="https://2012.igem.org/Team:UC_Davis/Project "><p>Project</p></a> <ul style="text-indent:-15px;list-style-image:none;list-style-type:none;color:#000000"><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Project">Project Overview</a> </li><li><a style="color:#000000 "  href="https://2012.igem.org/Team:UC_Davis/Project/Strain ">Modular Engineering</a></li><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Project/Catalyst ">Chassis Engineering</a></li><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Project/Protein_Engineering ">Protein Engineering</a></li> </ul> </li>
 +
 +
<li style="float:left ;margin:0 10px"><a href="https://2012.igem.org/Team:UC_Davis/Safety "> <p>Safety</p></a> <a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Safety "> Safety</a> </li>
 +
 +
<li style="float:left ;margin:0 10px;"><a href="https://2012.igem.org/Team:UC_Davis/Notebook "> <p>Notebook</p></a> <ul style="text-indent:-15px;list-style-image:none;list-style-type:none;color:#000000  "><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Notebook/Overview">Overview</a> </li><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Notebook/Protocols ">Protocols</a> </li><li><a style="color:#000000 " href="https://2012.igem.org/Team:UC_Davis/Notebook ">Notebook</a> </li> </ul> </li>
 +
 +
<li style="float:left ;margin:0 10px;"><a href="https://2012.igem.org/Team:UC_Davis/Data "> <p>Data </p><ul style="text-indent:-15px;list-style-image:none;
 +
list-style-type:none;color:#000000"><li>Data One </li><li>Data Two </li><li>Data Three </li></ul></a> </li>
 +
 +
<li style="float:left ;margin:0 10px"><a href="https://2012.igem.org/Team:UC_Davis/Attributions "> <p>Attribution </p></a><a style="color:#000000 "  href="https://2012.igem.org/Team:UC_Davis/Attributions "> Attribution</a></li>
 +
 +
<li style="float:left ;margin:0 10px"><a href="https://2012.igem.org/Main_Page "> <p>iGEM </p></a><a style="color:#000000 " href="https://2012.igem.org/Main_Page " > iGEM</a></li>
 +
</ul>
</div>
</div>
-
</div>
+
<!-- site map ends here -->
 +
</div>
 +
</div>
<!--  </div> -->
<!--  </div> -->
<script>if (window.runOnloadHook) runOnloadHook();</script>
<script>if (window.runOnloadHook) runOnloadHook();</script>
</div>
</div>
</div>
</div>
-
 
</body>
</body>
 +
<!-- footer starts here -->
 +
<br>
 +
<div id="footpage">
 +
<div class="printfooter">
 +
Retrieved from "<a href="https://2012.igem.org/Team:UC_Davis">https://2012.igem.org/Team:UC_Davis</a>"</div>
 +
<div id="catlinks"><div id='catlinks' class='catlinks catlinks-allhidden'></div></div> <!-- end content -->
 +
<div class="visualClear"></div>
 +
</div>
 +
    </div>
 +
 +
 +
<script>if (window.runOnloadHook) runOnloadHook();</script>
 +
</div>
 +
</div>
 +
<!-- footer ends here -->
</html>
</html>

Revision as of 21:14, 6 September 2012

Team:UC Davis - 2012.igem.org

UCDavis iGEM Tweets

Our Sponsors

Safety

Our team made sure to perform a thorough analysis of safety for all parts of our project, since safety for researchers, the public, and the environment are all important. We work within a Level 2 Biosafety Lab, as defined by the Laboratory Biosafety Manual (1). This facility is well equipped to work with all the strains of E. coli we used. We used MG1655, DH5α, and K12, all of which are Risk Level 1, meaning these strains pose no pathogenic risk to humans, and were completely contained within the lab.

We always made sure to make use of protective gear, such as wearing gloves at all times, masks when using UV lamps, and lab coats when appropriate. All of our waste was autoclaved on site as well, and all tools and glassware thoroughly cleaned after each use.

We worked with ethylene glycol during our experiments, which is toxic to the kidney, liver and central nervous system, as well as a skin and eye irritant. Obviously, we wanted to be very safe when using this chemical, so we made sure to consult with our advisers on how best to handle it. When using ethylene glycol, we always worked under a fume hood and wore lab coats. We had a separate waste bottle for all of the byproducts with ethylene glycol, so that the waste could be treated.

The University of California at Davis has a biosafety group, which heads the disposal and use of hazardous materials. They approve of our project and the procedures that we have followed. Further, they are excited for the potential that this project has, in terms of making the environment safer for inhabitants.

Our BioBricked parts do not raise any safety concerns, as long as the enzymes breaking down ethylene glycol are fully functional.

Our project, when properly functioning, should not have any harmful effect on the environment, researchers, or the public. We keep everything contained within the lab, and all of the plates and wastes are autoclaved to prevent all bacteria we use from spreading to the outside environment. If we were to escalate our project to an industrial level, everything would still be contained within the facility.

If we were to implement our bacteria in a landfill, chances are it could not survive past a couple generations and therefore would not be very toxic. However, if we were to imagine a more robust version of our strain created in the future, we would implement a kill switch, which has already been created using synthetic biology, to make sure we could stop it (2). We would also create a system similar to Imperial College London’s Gene Guard, which would stop to plastic-degrading plasmid from getting transferred to other bacteria (3). This would contain the plasmid within only our bacteria, so it would not get out of hand.

Our project could potentially be dangerous if our strain received a pathogenic plasmid by lateral gene transfer from other organisms. However, if this were a concern, our strain would be just a susceptible to pathogenicity as any other. If someone with malicious intent were to use our construct, perhaps the most they could do is release it into the water way and have it degrade plastic pipes made of PET, or modify it to degrade other plastics in addition to PET and degrade something useful. Cutinase, the first enzyme in our degradation pathway, functions optimally at 50° C, and the water in our pipes would most likely not be at that temperature. Also, it is unlikely the strain would survive under conditions such as this temperature and being in an aquatic environment.


Questions

1. Would any of your project ideas raise safety issues in terms of: researcher safety, public safety, or environmental safety?

Our projects involves ethylene glycol, a moderately toxic substance, which is eventually oxidized to oxalic acid – a toxic substance that affects the central nervous system via the liver. Because ethylene glycol must be ingested to pose a problem, researchers take extra precaution to make sure there are no splashes of ethylene glycol in the laboratory and the wastes will be disposed of in the appropriate hazardous waste receptacles. Ethylene glycol can also be a mild irritant if it comes in contact with the skin or if it is inhaled, so researchers wear eye protection as well as gloves and lab coats, and always work with ethylene glycol in the confine of a fume hood.
In the environment, ethylene glycol is degraded by hydroxyl radicals and in sewage sludge, it is readily biodegradable. However,ethylene glycol can potentially be toxic within waterways so to ensure environmental safety the team has taken extra precautions to dispose of ethylene glycol safely. Additionally, we have considered how to deal with ethylene glycol by designing a circuit in which we produce enzymes that will degrade ethylene glycol into glycoaldehyde and then glycolate. The glycolate has the potential to be turned in to oxaloacetate, a metabolic intermediate.


2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes, did you document these issues in the Registry? How did you manage to handle the safety issue? How could other teams learn from your experience?

Our BioBrick parts this year do not raise any serious safety issues. However, One part, the LC-Cutinase gene BBa_K936000, encodes an enzyme that breaks down PET into ethylene glycol, a moderately toxic substance. This has been noted on its registry page. The safety management procedures have been discussed in the previous question’s answer. The novel part that we are submitting to the Registry is the cutinase gene, in BioBrick format. This is a lipolytic enzyme that does not pose a threat to humans.


3. Is there a local biosafety group, committee, or review board at your institution? If yes, what does your local biosafety group think about your project? If no, which specific biosafety rules or guidelines do you have to consider in your country?

The University of California at Davis has a biosafety group, which heads the disposal and use of hazardous materials. They approve of our project and the procedures that we have followed. Further, they are excited for the potential that this project has, in terms of making the environment safer for inhabitants.


4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?

Safety issues could be headed by a designated safety monitor in each iGEM group. This set-up allows all of the teams to have a person who will always make sure that the safest procedures are being followed at all times. Also, the production of potentially hazardous materials should always be controlled by an inducible promoter, so that it may be stopped at any time. The different parts can be made safer by making the parts work only with BioBrick assembly units and nothing else that exists in nature.

References

1. Laboratory Biosafety Manual. The World Health Organization. 2004. 3rd edition.
2. Callura, Jared, Dwyer, Daniel, Isaacs, Farren, Cantor, Charles, Collins, James. “Tracking, tuning, and terminating microbial physiology using synthetic riboregulators”. PNAS vol. 107 no. 36, pp. 15898-15903. Web. 13 July 2010.
3. “Gene Overview”, Imperial College London iGEM Wiki 2011.

Retrieved from "http://2012.igem.org/Team:UC_Davis/Safety"