Team:UCSF/Violacein Results

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<img align="left" style="margin-bottom:8px; width:755px;height:410px; padding:0;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/violacein/100212vio_std_cf.jpg">
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<regulartext> During our experiments we worked with several different strains of <i>E. coli</i>, each containing plasmids with different violacein constructs:
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<li>Strain 1: pcdfDuet+VioABE</li>
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<li>Strain 2:pcdfDuet+VioDC</li>
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<li>Strain 3: pcdfDuet:VioABE+VioDC (full operon)</li>
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The details of growth and sample analysis can be found on our protocols page.

Revision as of 16:23, 3 October 2012

Production of Violacein by an E. coli Co-Culture


Before we started our experiments, we obtained a violacein standard from Sigma-Aldrich,Item:V9389-1MG . We used this standard to measure the absorbance of violacein at varying concentrations, obtaining the standard curve shown below.
During our experiments we worked with several different strains of E. coli, each containing plasmids with different violacein constructs:

  • Strain 1: pcdfDuet+VioABE
  • Strain 2:pcdfDuet+VioDC
  • Strain 3: pcdfDuet:VioABE+VioDC (full operon)

    • The details of growth and sample analysis can be found on our protocols page.

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