Team:UCSF/Protocols

From 2012.igem.org

(Difference between revisions)
 
(14 intermediate revisions not shown)
Line 4: Line 4:
<link rel="stylesheet" type="text/css" href="http://tinyurl.com/twilightsparkles"/>
<link rel="stylesheet" type="text/css" href="http://tinyurl.com/twilightsparkles"/>
 +
 +
<style>
<style>
-
#rightcontent {width:755px; float:left; background-color: #F5F5F5; margin-left: 8px;  margin-top:10px;}
+
#mission {width: 500px; float:left; background-color: #F5F5F5; margin-left:8px; padding: 10px; margin-top:8px;}
 +
#opensource {width:306px; float:left; background-color: #F5F5F5; margin-left:8px; padding: 10px; margin-top:8px;}
 +
#rightcontent {width:755px; float:right; background-color: #F5F5F5; margin-left: 8px;  margin-top:10px;}
 +
#photos {width:155px; float:left; background-color: #F5F5F5; margin-left: 8px;  margin-top:10px;}
 +
#description{width:450px; height:110px;float:left; background-color: #F5F5F5; margin-left: 8px;  margin-top:10px;}
 +
#flickr{width:755px; float:right;}
 +
#leftcolumntotal{width:200px; height:900px; float: left; margin-top:0px;}
 +
#rightcolumntotal{width:200px; height:900px; float: right; margin-top:0px;}  
</style>
</style>
Line 19: Line 28:
<regulartext>  
<regulartext>  
<ul>
<ul>
-
<li>Primer Design</li>
+
<li><a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Protocols/Creating%20Primers%20on%20ApE.doc">Primer Design for Gibson and Seamless Cloning</a> <br></li>
-
<li>Phusion PCR Protocol</li>
+
-
<li>Gibson Assembly Protocol</li>
+
-
<li>Seamless Cloning Protocol</li>
+
-
<li>Colony PCR Protocol</li>
+
-
<li><i>E. coli</i> Transformation</li>
+
-
</ul> <br>
+
-
<h3red>Violacein Production Protocols</h3red> <p><br>
+
<li><a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Protocols/PCR%20Phusion.pdf">PCR with Phusion Polymerase</a> <br></li>
-
<h3red>Auxotroph System Protocols</h3red> <p>
+
<li><a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Protocols/Gibson%20Assembly.pdf">Gibson Assembly</a> <br></li>
-
<ul>
+
 
-
<li>Growing in M9</li>
+
 
-
<li>Growing Monocultures</li>
+
<li><a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Protocols/geneart_seamless_cloning_and_assembly_man-1.pdf">Seamless Cloning Protocol</a> <br></li>
-
<li>Co-culture Experiments</li>
+
 
-
<br>
+
<li><a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Protocols/Ecoli%20Transformation.pdf"><i>E. coli</i> Transformation</a> <br></li>
 +
 
 +
</ul> <br>
-
<h3red>Toxin/Antitoxin System Protocols</h3red><br>
+
<regulartext1> The above protocols were used in the development of each part of our project. </regulartext1> <p>
 +
<img align="center" style="margin-bottom:0px; width: 555px; margin-top:-3px; padding:0;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/Background/All%20Projects%20Slide.jpg">

Latest revision as of 21:31, 3 October 2012

General Cloning Protocols


The above protocols were used in the development of each part of our project.