Revision as of 08:18, 5 September 2012 by Jakobmatthes (Talk | contribs)


  • Project idea. The initial basic project idea (to detect and/or degrade steroids) was proposed by the student team.
  • System design. The overall design (including selection of vectors, promoters and genes) was conducted in close cooperation with Prof. Jansen. Primer design was performed with students and Dr. Apel. At all times students were present. Each step was explained to all students so everyone has knowledge of the full process.
  • Lab supplies. LB medium was initially prepared by the student team to learn the protocol. Later on, LB was provided by the host lab. SOB and TAE buffer was always prepared by the students. Agar plates were produced by the students with exception of the first batch which was provided by the host lab.
  • Sequencing. Sequencing was performed by an external provider. All sequencing results were evaluated by the students.