Team:Tokyo Tech/Projects/PHAs/detail/index.htm
From 2012.igem.org
(Difference between revisions)
(→A .PHB production on colonies and preparation before confirmation with Nile red under UV) |
(→Protocol) |
||
(3 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
{{tokyotechcss}} | {{tokyotechcss}} | ||
- | |||
{{tokyotechmenubar}} | {{tokyotechmenubar}} | ||
+ | <br><br> | ||
+ | |||
<div class="whitebox"> | <div class="whitebox"> | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | + | <div id="tokyotech" style=" font:bold ;left ; font-size: 15px; color: #000000; padding: 10px;"> | |
- | + | =Production trial of PHAs by past teams= | |
- | + | ||
[[https://2012.igem.org/Team:Tokyo_Tech/Projects/PHAs/index.htm#Construction_of_phaC1-A-B1_in_Biobrick_format Back to "Construction of phaC1-A-B1 in Biobrick format"]] | [[https://2012.igem.org/Team:Tokyo_Tech/Projects/PHAs/index.htm#Construction_of_phaC1-A-B1_in_Biobrick_format Back to "Construction of phaC1-A-B1 in Biobrick format"]] | ||
Line 132: | Line 121: | ||
[[https://2012.igem.org/Team:Tokyo_Tech/Projects/PHAs/index.htm#Construction_of_phaC1-A-B1_in_Biobrick_format Back to "Construction of phaC1-A-B1 in Biobrick format"]] | [[https://2012.igem.org/Team:Tokyo_Tech/Projects/PHAs/index.htm#Construction_of_phaC1-A-B1_in_Biobrick_format Back to "Construction of phaC1-A-B1 in Biobrick format"]] | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- |
Latest revision as of 09:23, 22 October 2012
Production trial of PHAs by past teams
[Back to "Construction of phaC1-A-B1 in Biobrick format"]
We introduce some attempts in the past iGEM to show how great our work is in iGEM.
Part number | Description | States | experience |
BBa_K338003 | PHA Synthase Composite, Part 1/2 | Planning | |
BBa_K338004 | PHA Synthase Composite, Part 1/2 | Available | None |
They couldn’t prove that their engineered bacteria produced PHB according to the team wiki.
Part number | Description | States | experience |
BBa_K342001 | Pha C (poly-β-hydroxybutyrate polymerase) | Available | None |
They registered and submitted a part, which contains only phaC sequence, as worked. But the team wiki shows that the part doesn’t meet the standard which is required in iGEM because they couldn’t cut their part with XbaI.
Part number | Description | States | experience |
BBa_K282000 | phaAB | Available | None |
Part number | Description | States | experience |
BBa_K089001 | phaA gene | Planning | |
BBa_K089002 | phaB gene | Planning | |
BBa_K089003 | phaC gene | Planning |
Part number | Description | States | experience |
BBa_K125801 | RBS-phaA | Available | None |
BBa_K125802 | RBS-phaB | Planning | |
BBa_K125803 | RBS-phaC | Available | None |
BBa_K125804 | RBS-phaE | Available | None |
BBa_K125501 | phaA BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1994) | Available | None |
BBa_K125502 | phaB BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1994) | Available | None |
BBa_K125503 | phaC BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1994) | Available | None |
BBa_K125504 | phaE BioPlastic polyhydroxybutyrate synthesis pathway (origin PCC6803 slr1994) | Available | None |
Part number | Description | States | experience |
BBa_K156031 | RBS + phaA + double terminator | Available | None |
BBa_K156033 | RBS + phaB1 + double terminator | Available | None |
BBa_K156034 | RBS + phaC1 + double terminator | Available | None |
BBa_K156012 | phaA (acetyl-CoA acetyltransferase) | Available | None |
BBa_K156013 | phaB1 (acetyacetyl-CoA reductase) | Available | None |
BBa_K156014 | phaC1 (Poly(3-hydroxybutyrate) polymerase) | Available | None |
BBa_K156021 | Promoter + RBS + phaA + double terminator | Available | None |
BBa_K156018 | Promoter + RBS + phaA | Planning | |
BBa_K156019 | Promoter + RBS + phaB1 | Planning | |
BBa_K156020 | Promoter + RBS + phaC1 | Planning | |
BBa_K156022 | Promoter + RBS + phaB1 + double terminator | Planning | |
BBa_K156023 | Promoter + RBS + phaC1 + double terminator | Planning |