Team:Tokyo Tech/Experiment/PHB2

From 2012.igem.org

(Difference between revisions)
Line 4: Line 4:
<div class="whitebox">
<div class="whitebox">
-
=P(3HB) production by <I>E.coli</I> & Confirmation of P(3HB)=
+
=D Make purified P(3HB) sheets=
-
aaa
+
 
 +
We made P(3HB) sheets. To make the sheets, we cultured <I>E.coli</I> JM109 in erlenmeyer flasks at 37°C for 72h. Contact angle is an indicator to represent the strength of the water-repellent. The angle shows the physical properties, especially surface tension.
 +
When contact angle of sheets is larger than 90°, from Young equation, the sheets would have more strong water-repellent by increasing real surface area. Contact angle of P(3HB) sheets is about 100° from literature data.
 +
[[http://2012.igem.org/Team:Tokyo_Tech/Experiment/PHB#D_Polymer_extraction_and_purification Protocol]]
 +
 
 +
 
 +
[[File:tokyotech PHB6.png|600px|thumb|center|Fig2-2-4-4-1, P(3HB) sheet]]

Revision as of 16:34, 26 October 2012

bar



D Make purified P(3HB) sheets

We made P(3HB) sheets. To make the sheets, we cultured E.coli JM109 in erlenmeyer flasks at 37°C for 72h. Contact angle is an indicator to represent the strength of the water-repellent. The angle shows the physical properties, especially surface tension. When contact angle of sheets is larger than 90°, from Young equation, the sheets would have more strong water-repellent by increasing real surface area. Contact angle of P(3HB) sheets is about 100° from literature data. [Protocol]


Fig2-2-4-4-1, P(3HB) sheet