http://2012.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Experiment/C6&feed=atom&action=historyTeam:Tokyo Tech/Experiment/C6 - Revision history2024-03-28T09:02:46ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Experiment/C6&diff=292246&oldid=prevTakuo: /* Protocol */2012-10-26T23:43:20Z<p><span class="autocomment">Protocol</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. collect liquid culture</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1. collect liquid culture</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1.1 Prepare overnight culture of inducer cell at 37°C for 12hours.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>1.1 Prepare overnight culture of inducer cell at 37°C for 12hours.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2 Reporter assay</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2 Reporter assay</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2.1 Prepare overnight culture of reporter cell at 37°C for 12hours.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>2.1 Prepare overnight culture of reporter cell at 37°C for 12hours.</div></td></tr>
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</table>Takuohttp://2012.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Experiment/C6&diff=291281&oldid=prevTakuo: /* Construction of the 3OC6HSL-dependent 3OC12HSL production module */2012-10-26T22:49:37Z<p><span class="autocomment">Construction of the 3OC6HSL-dependent 3OC12HSL production module</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Construction of the 3OC6HSL-dependent 3OC12HSL production module=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Construction of the 3OC6HSL-dependent 3OC12HSL production module=</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For construction of the 3OC6HSL-dependent 3OC12HSL production module, we firstly constructed a new part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]). Plux-LasI cell is an engineered <I>E.coli</I> that contains a 3OC6HSL-dependent LasI generator and a constitutive LuxR generator. As a 3OC6HSL-dependent LasI generator, we constructed a new Biobrick part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]) by combining Plux promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For construction of the 3OC6HSL-dependent 3OC12HSL production module, we firstly constructed a new part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]). Plux-LasI cell is an engineered <I>E.coli</I> that contains a 3OC6HSL-dependent LasI generator and a constitutive LuxR generator. As a 3OC6HSL-dependent LasI generator, we constructed a new Biobrick part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]) by combining Plux promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:positivefeedbackassay23tokyotech.png|450px|thumb|right|Fig2-1-3-1-4, 3OC6HSL-dependent 3OC12HSL production]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:positivefeedbackassay23tokyotech.png|450px|thumb|right|Fig2-1-3-1-4, 3OC6HSL-dependent 3OC12HSL production]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Fig2-1-3-1-4 shows fluorescence intensities by the reporter cells dependent on different conditions. Only when the supernatant of condition B was used, the fluorescence intensity of the Las reporter cell increased, while the supernatants of other three conditions did not affect. Comparing the results of the condition A and B, it can be said that with the induction of 3OC6HSL to Plux-LasI cell, the fluorescence intensity of the <del class="diffchange diffchange-inline">LasI </del>reporter cell increased by 20-folds. This result indicates that Plux-LasI cell produced 3OC12HSL in response to 3OC6HSL induction by the function of Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Fig2-1-3-1-4 shows fluorescence intensities by the reporter cells dependent on different conditions. Only when the supernatant of condition B was used, the fluorescence intensity of the Las reporter cell increased, while the supernatants of other three conditions did not affect. Comparing the results of the condition A and B, it can be said that with the induction of 3OC6HSL to Plux-LasI cell, the fluorescence intensity of the <ins class="diffchange diffchange-inline">Las </ins>reporter cell increased by 20-folds. This result indicates that Plux-LasI cell produced 3OC12HSL in response to 3OC6HSL induction by the function of Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>). From this experiment, we confirmed that a new part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>). From this experiment, we confirmed that a new part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>) synthesized enough concentration of 3OC12HSL to induce the Las reporter cell. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>) synthesized enough concentration of 3OC12HSL to induce the Las reporter cell. </div></td></tr>
</table>Takuohttp://2012.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Experiment/C6&diff=288388&oldid=prevTakuo at 19:26, 26 October 20122012-10-26T19:26:33Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><div id="tokyotech" style=" font:Arial ;left ; font-size: 15px; color: #000000; padding: 30px;"> </ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For construction of the 3OC6HSL-dependent 3OC12HSL production module, we firstly constructed a new part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]). Plux-LasI cell is an engineered <I>E.coli</I> that contains a 3OC6HSL-dependent LasI generator and a constitutive LuxR generator. As a 3OC6HSL-dependent LasI generator, we constructed a new Biobrick part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]) by combining Plux promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For construction of the 3OC6HSL-dependent 3OC12HSL production module, we firstly constructed a new part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]). Plux-LasI cell is an engineered <I>E.coli</I> that contains a 3OC6HSL-dependent LasI generator and a constitutive LuxR generator. As a 3OC6HSL-dependent LasI generator, we constructed a new Biobrick part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]) by combining Plux promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]</div></td></tr>
</table>Takuohttp://2012.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Experiment/C6&diff=288370&oldid=prevTakuo at 19:25, 26 October 20122012-10-26T19:25:49Z<p></p>
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</table>Takuohttp://2012.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Experiment/C6&diff=283414&oldid=prevTakuo: /* Construction of the 3OC6HSL-dependent 3OC12HSL production module */2012-10-26T11:35:05Z<p><span class="autocomment">Construction of the 3OC6HSL-dependent 3OC12HSL production module</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>). From this experiment, we confirmed that a new part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>). From this experiment, we confirmed that a new part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>) synthesized enough concentration of 3OC12HSL to induce the Las reporter cell. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>) synthesized enough concentration of 3OC12HSL to induce the Las reporter cell. </div></td></tr>
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</table>Takuohttp://2012.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Experiment/C6&diff=283411&oldid=prevTakuo: /* Construction of the 3OC6HSL-dependent 3OC12HSL production module */2012-10-26T11:34:34Z<p><span class="autocomment">Construction of the 3OC6HSL-dependent 3OC12HSL production module</span></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[File:positivefeedbackassay21tokyotech.png|550px|thumb|right|Fig2-1-3-1-2, Las sender strain and Las reporter strain]]</del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For construction of the 3OC6HSL-dependent 3OC12HSL production module, we firstly constructed a new part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]). Plux-LasI cell is an engineered <I>E.coli</I> that contains a 3OC6HSL-dependent LasI generator and a constitutive LuxR generator. As a 3OC6HSL-dependent LasI generator, we constructed a new Biobrick part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]) by combining Plux promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>For construction of the 3OC6HSL-dependent 3OC12HSL production module, we firstly constructed a new part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]). Plux-LasI cell is an engineered <I>E.coli</I> that contains a 3OC6HSL-dependent LasI generator and a constitutive LuxR generator. As a 3OC6HSL-dependent LasI generator, we constructed a new Biobrick part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]) by combining Plux promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>). As a constitutive LuxR generator, we used Ptet-LuxR ([http://partsregistry.org/wiki/index.php?title=Part:BBa_S03119 BBa_S03119]). By introducing Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>). As a constitutive LuxR generator, we used Ptet-LuxR ([http://partsregistry.org/wiki/index.php?title=Part:BBa_S03119 BBa_S03119]). By introducing Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>. As the negative control of 3OC12HSL production, we prepared 3OC12HSL non-producer cell (ΔP-LasI cell) that contains, in addition to Ptet-LuxR ([http://partsregistry.org/wiki/index.php?title=Part:BBa_S03119 BBa_S03119]), promoterless-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]) </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>. As the negative control of 3OC12HSL production, we prepared 3OC12HSL non-producer cell (ΔP-LasI cell) that contains, in addition to Ptet-LuxR ([http://partsregistry.org/wiki/index.php?title=Part:BBa_S03119 BBa_S03119]), promoterless-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]) </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>instead of Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]) (Fig2-1-3-1-2). </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>instead of Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]) (Fig2-1-3-1-2). </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[File:positivefeedbackassay21tokyotech.png|550px|thumb|center|Fig2-1-3-1-2, Las sender strain and Las reporter strain]]</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The ⊿P-LasI cell does not produce 3OC12HSL even though 3OC6HSL exist. The supernatants of the cultures of these modules were used as the inducer in the reporter assay (Fig2-1-3-1-3).</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">The ⊿P-LasI cell does not produce 3OC12HSL even though 3OC6HSL exist. The supernatants of the cultures of these modules were used as the inducer in the reporter assay (Fig2-1-3-1-3). We prepared four conditions as follow.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:positivefeedbackassay22tokyotech.png|500px|thumb|<ins class="diffchange diffchange-inline">center</ins>|Fig2-1-3-1-3, How to perform 3OC6HSL-dependent 3OC12HSL production assay]]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:positivefeedbackassay22tokyotech.png|500px|thumb|<del class="diffchange diffchange-inline">right</del>|Fig2-1-3-1-3, How to perform 3OC6HSL-dependent 3OC12HSL production assay]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br><br></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">We prepared four conditions as follow.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A) Culture containing Plux-LasI cell without 3OC6HSL induction</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A) Culture containing Plux-LasI cell without 3OC6HSL induction</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the reporter assay, we used a Las reporter strain that contains Ptrc-LasR and Plas-GFP ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K649001 BBa_K649001]). Also, a reporter cell that expresses GFP constitutively and a reporter cell that does not express GFP were used as the positive control and the negative control, respectively.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the reporter assay, we used a Las reporter strain that contains Ptrc-LasR and Plas-GFP ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K649001 BBa_K649001]). Also, a reporter cell that expresses GFP constitutively and a reporter cell that does not express GFP were used as the positive control and the negative control, respectively.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"> </del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:positivefeedbackassay23tokyotech.png|450px|thumb|right|Fig2-1-3-1-4, 3OC6HSL-dependent 3OC12HSL production]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:positivefeedbackassay23tokyotech.png|450px|thumb|right|Fig2-1-3-1-4, 3OC6HSL-dependent 3OC12HSL production]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Fig2-1-3-1-4 shows fluorescence intensities by the reporter cells dependent on different conditions. Only when the supernatant of condition B was used, the fluorescence intensity of the Las reporter cell increased, while the supernatants of other three conditions did not affect. Comparing the results of the condition A and B, it can be said that with the induction of 3OC6HSL to Plux-LasI cell, the fluorescence intensity of the LasI reporter cell increased by 20-folds. This result indicates that Plux-LasI cell produced 3OC12HSL in response to 3OC6HSL induction by the function of Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Fig2-1-3-1-4 shows fluorescence intensities by the reporter cells dependent on different conditions. Only when the supernatant of condition B was used, the fluorescence intensity of the Las reporter cell increased, while the supernatants of other three conditions did not affect. Comparing the results of the condition A and B, it can be said that with the induction of 3OC6HSL to Plux-LasI cell, the fluorescence intensity of the LasI reporter cell increased by 20-folds. This result indicates that Plux-LasI cell produced 3OC12HSL in response to 3OC6HSL induction by the function of Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>). From this experiment, we confirmed that a new part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>). From this experiment, we confirmed that a new part Plux-LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>) synthesized enough concentration of 3OC12HSL to induce the Las reporter cell. <del class="diffchange diffchange-inline">[[https://2012.igem.org/Team:Tokyo_Tech/Projects/positive_feedback_assay/index.htm#3OC6HSL-dependent_3OC12HSL_production_module Protocol]]</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>) synthesized enough concentration of 3OC12HSL to induce the Las reporter cell. </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Materials & Methods=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Materials & Methods=</div></td></tr>
</table>Takuohttp://2012.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Experiment/C6&diff=283380&oldid=prevTakuo at 11:29, 26 October 20122012-10-26T11:29:28Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Construction of the 3OC6HSL-dependent 3OC12HSL production module=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Construction of the 3OC6HSL-dependent 3OC12HSL production module=</div></td></tr>
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</table>Takuohttp://2012.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Experiment/C6&diff=274552&oldid=prevTakuo: /* 1.Construction */2012-10-23T11:52:49Z<p><span class="autocomment">1.Construction</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>pSB6A1-Ptrc-LasR / pSB3K3-ΔP-GFP (JM2.300)…negative control</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>pSB6A1-Ptrc-LasR / pSB3K3-ΔP-GFP (JM2.300)…negative control</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:<del class="diffchange diffchange-inline">positivefeedbackassay9tokyotech</del>.png|300px|center]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:<ins class="diffchange diffchange-inline">positivefeedbackassay10tokyotech</ins>.png|300px|center]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>pSB6A1-Ptrc-LasR / pSB3K3-pλ-GFP (JM2.300)…positive control</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>pSB6A1-Ptrc-LasR / pSB3K3-pλ-GFP (JM2.300)…positive control</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:<del class="diffchange diffchange-inline">positivefeedbackassay10tokyotech</del>.png|300px|center]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:<ins class="diffchange diffchange-inline">positivefeedbackassay9tokyotech</ins>.png|300px|center]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==2.Strain==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==2.Strain==</div></td></tr>
</table>Takuohttp://2012.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Experiment/C6&diff=272223&oldid=prevTakuo: /* Materials & Methods */2012-10-21T05:04:47Z<p><span class="autocomment">Materials & Methods</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Materials & Methods=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Materials & Methods=</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[https://2012.igem.org/Team:Tokyo_Tech/Project#Construction_of_the_3OC6HSL-dependent_3OC12HSL_production_module <del class="diffchange diffchange-inline">back </del>to the project page "3OC12HSL production module"]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[https://2012.igem.org/Team:Tokyo_Tech/Project#Construction_of_the_3OC6HSL-dependent_3OC12HSL_production_module <ins class="diffchange diffchange-inline">Go </ins>to the project page "<ins class="diffchange diffchange-inline">Construction of the 3OC6HSL dependent </ins>3OC12HSL production module"]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==1.Construction==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==1.Construction==</div></td></tr>
</table>Takuohttp://2012.igem.org/wiki/index.php?title=Team:Tokyo_Tech/Experiment/C6&diff=272216&oldid=prevTakuo: /* Materials & Methods */2012-10-21T05:02:04Z<p><span class="autocomment">Materials & Methods</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Materials & Methods=</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=Materials & Methods=</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[https://2012.igem.org/Team:Tokyo_Tech/Project#Construction_of_the_3OC6HSL-dependent_3OC12HSL_production_module back to the project page "3OC12HSL production module"]]</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==1.Construction==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>==1.Construction==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="tokyotech" style=" font:bold ;left ; font-size: 20px; color: #1E90FF; padding: 10px;"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><div id="tokyotech" style=" font:bold ;left ; font-size: 20px; color: #1E90FF; padding: 10px;"></div></td></tr>
</table>Takuo