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A Project over view

A love story contains several processes. Two people fall in love and their love burning wildly. However, no forever exists in the world, in most occasions, love will eventually burn to only a pile of ashes of the last remaining wind drift away. In our project, we have recreated the story of "Romeo & Juliet" by Shakespeare vividly by two kinds of Escherichia coli. We aim to generate a circuit involving regulatory mechanism ofrather than commonly-used negative feedback to control the fate of E.coli by signaling between two types of E.coli. Besides, Rose represents love. We are the first iGEM group ever to synthesize PHA (a kind of bio-plastics) which to represent rose by using our new biobrick part.

Ⅰ cell-cell communication

"Romeo and Juliette" is the dramas by dramatist William Shakespeare of England. The stage is city Verona, Italy in the 14th century. Romeo met Juliette. Two persons fall to love instantly. In this project, we will recreate the love story of "Romeo & Juliette".

In this project, we will recreate the love story of "Romeo&Juliette".

Ⅰ-1 story

We make our cute E.coli play “Romeo and Juliet” which is one of Shakespeare’s most famous plays. In this project, we define the signal which E.coli gives out as the romantic feeling of Romeo and Juliet. The story which we reproduce is divided into four scenes. (Scene 1) Romeo and Juliet encountered and they fell in love with each other. (Scene 2) However, providence lane person, this period of pure love might never broom since they were born in two hostile families. In order to keep their relationship, Juliet was planning to pretend died by drinking the poison so that when she woke up there will be no obstacle between their great loves. She planned and she did. (Scene 3)However, this new was not conveyed to Romeo in time. Romeo misunderstood Juliet’s death and committed suicide. (Scene 4) When Juliet woke up, it was not a beautiful future but Romeo’s death that is waiting for her. Juliet felt so sad and she followed Romeo by stabbing herself with a dirk.

Gene Circuit

Ⅰ-2 Positive Feedback of cell-cell communication

Ⅰ-3 Modeling

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Ⅱ Organic synthesis of PHA

There is what of the famous scene of "Romeo and Juliette"

PHA is a kind of bio-plastic produced by microbes.It's standard extract can be got after being dissolved in chloroform. In this project, we will recreate the rose come out in the lines of the famous drama'Romeo and Juliet'by the synthesis of PHA. PHA can be detected by Nile Red reagent by being dyed red, which looks exactly like rose.