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Team:Tokyo Tech
From 2012.igem.org
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| - | In | + | In this project, instead of applying general regulatory mechanism of negative feedback, |
| + | we will generate a circuit including positive feedback to control the fate of E.coli | ||
| + | by signaling between them. Together with the synthesis of PHA, | ||
| + | we will recreate the rose like love story of "Romeo&Juliette". | ||
Revision as of 08:06, 2 September 2012
Love is like a butterfly.It goes where it pleases and it pleases where it goes. Summer comes and the season of love comes! However, we want to do something special in this summer.We decide to make E.coli love like human!E.Coli will be starring in 「Romeo and Juliet」which is among Shakespeare's most popular plays.
Contents |
Chemical synthesis of PHA
There is what of the famous scene of "Romeo and Juliette" is such to one.
JULIET: O Romeo, Romeo! wherefore art thou Romeo? Deny thy father and refuse thy name; Or, if thou wilt not, be but sworn my love, And I'll no longer be a Capulet.
ROMEO: Shall I hear more, or shall I speak at this?
JULIET: 'Tis but thy name that is my enemy; Thou art thyself, though not a Montague. What's Montague? it is nor hand, nor foot, Nor arm, nor face, nor any other part Belonging to a man. O, be some other name! What's in a name? that which we call a rose By any other name would smell as sweet.
PHA is a kind of bio-plastic produced by microbes.It's standard extract can be got after being dissolved in chloroform. In this project, we will reproduce the rose come out in the lines of the famous drama'Romeo and Juliet'by the synthesis of PHA. PHA can be detected by Nile Red reagent by being dyed red, which looks exactly like rose.
cell-cell communication
In this project, instead of applying general regulatory mechanism of negative feedback, we will generate a circuit including positive feedback to control the fate of E.coli by signaling between them. Together with the synthesis of PHA, we will recreate the rose like love story of "Romeo&Juliette".
