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Revision as of 15:31, 23 June 2012
Saturday, 23th June
Quick Change mutagenis to remove NgoMIV from pYES2
Investigator: Ingmar, Volker
PCR reaction batch
| |
| volume | substance |
| 2.5 µl | 10x Pfu Ultra II buffer |
| 4 µl | Plasmid P7 pYes2_RFC25 MCS template |
| 0.5 µl | 1:10 dilution of O38 (10 pmol/µL) |
| 0.5 µl | 1:10 dilution of O39 ((10 pmol/µL) |
| 17 µl | ddH2O |
| 0.5 µl | dNTP mix |
| 0.5 µl | Pfu Turbo DNA polymerase (2.5 U / µl) |
