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Team:TU Darmstadt/Protocols
From 2012.igem.org
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_Periplasmatic_Proteins Purification of Periplasmatic Proteins] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_Periplasmatic_Proteins Purification of Periplasmatic Proteins] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page] | ||
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== Material Science == | == Material Science == | ||
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== Miscellaneous == | == Miscellaneous == | ||
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=== Staining === | === Staining === | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Antibody_staining Antibody staining] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Antibody_staining Antibody staining] | ||
Latest revision as of 21:45, 26 September 2012
Protocols
The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012. The corresponding materials can be found in the materials section.
Contents |
In vivo
Escherichia coli
- Bacterial cell culture
- Bacterial transformation aka TRAFO
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Electrocompetent cells
- Electroporation
- Glycerine Stocks
- Heat Shock Transformation
- Miniprep / Fermentas kit
- Miniprep / Promega kit
- Miniprep / Qiagen kit
- Protein Expression Est13
- Protein Expression FsC
- Western blot
Comamonas testosteroni
In vitro
Nucleic acids
DNA
- Agarose gel electrophoresis
- Ammonium acetate precipitation
- Cloning
- Dephosphorylation
- DNA Ligation
- DNA Ligation à la group transport
- DNA Quantification / NanoDrop
- Miniprep
- Mutagenic PCR
- PCR
- Purification of DNA
- Restriction digest
- Salting Out
- Sequencing DNA
Protein
- Activity assay
- Affinity chromatography
- Immobilized metal ion affinity chromatography (IMAC)
- pNP-Assay Bacteria
- pNP-Assay Enzyme
- Proteinpurification
- Purification of Periplasmatic Proteins
- SDS-Page
Material Science
- Synthesis of paranitrophenylesters with acyl chlorides in presence of triethylamine
- Synthesis of polyethylene terephthalate in presence of sulfuric acid
- Surface analysis of polyethylene terephthalate with atomic force microscopy
Miscellaneous
Staining
Analytics
- TEM-EDX Measurements
- GC-MS Measurements
- Preparation of Escherichia Coli for the energy dispersive X-ray spectroscopy (EDX)
ILDs / Improvised Lab Devices
- x bp Primer Designer
- Enhancing a thermo cyclers cooling capabilites
- Gel pocket size reduction
- Growing iGEMlers
