Team:TU Darmstadt/Protocols

From 2012.igem.org

(Difference between revisions)
(Miscellaneous)
(Cell culture)
 
(31 intermediate revisions not shown)
Line 27: Line 27:
    <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li>
    <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li>
    <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li>
    <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li>
-
<li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a><ul>
+
<li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a></li>   
-
    <li><a href="/Team:TU_Darmstadt/Human_Practice/Panel_Discussion " title="Panel Discussion">Panel Discussion</a></li>
+
-
    <li><a href="/Team:TU_Darmstadt/Human_Practice/Symposia" title="Symposia">Symposia</a></li>
+
-
    <li><a href="/Team:TU_Darmstadt/Human_Practice/Classes" title="Classes">Classes</a></li></ul></li>   
+
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul>
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul>
    <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li>
    <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li>
Line 53: Line 50:
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electrocompetent_cells Electrocompetent cells]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electrocompetent_cells Electrocompetent cells]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Electroporation Electroporation]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Engineering_BioBrick_vectors_from_BioBrick_parts/Colony_PCR Engineering BioBrick vectors from BioBrick parts/Colony PCR]
 
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Glycerine_stock Glycerine Stocks]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Glycerine_stock Glycerine Stocks]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Heat_Shock_Transformation Heat Shock Transformation]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Heat_Shock_Transformation Heat Shock Transformation]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/In-fusion_BioBrick_assembly In-fusion BioBrick assembly]
 
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep#Fermentas_kit Miniprep / Fermentas kit]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep#Fermentas_kit Miniprep / Fermentas kit]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep#Promega_kit Miniprep / Promega kit]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep#Promega_kit Miniprep / Promega kit]
Line 62: Line 57:
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_Expression#Protein_Expression_Est13 Protein Expression Est13]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_Expression#Protein_Expression_Est13 Protein Expression Est13]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_Expression#Protein_Expression_FsC Protein Expression FsC]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_Expression#Protein_Expression_FsC Protein Expression FsC]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Transformation_of_chemically_competent_cells Transformation of chemically competent cells]
 
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Western_Blot Western blot]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Western_Blot Western blot]
=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Comamonas_testosteroni Comamonas testosteroni] ===
=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Comamonas_testosteroni Comamonas testosteroni] ===
* [http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/engl_Opening.pdf Reviving a freez dried cell culture]
* [http://www.dsmz.de/fileadmin/Bereiche/Microbiology/Dateien/Kultivierungshinweise/engl_Opening.pdf Reviving a freez dried cell culture]
-
* ...
 
== In vitro ==
== In vitro ==
Line 73: Line 66:
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR_techniques PCR techniques]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR_techniques PCR techniques]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SOE-PCR SOE-PCR]
+
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR#SOE_PCR SOE-PCR]
 +
 
=== [http://en.wikipedia.org/wiki/DNA DNA] ===
=== [http://en.wikipedia.org/wiki/DNA DNA] ===
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Agarose_gel_electrophoresis Agarose gel electrophoresis]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Ammonium_sulfate_precipitation Ammonium sulfate precipitation]
+
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Ammonium_sulfate_precipitation Ammonium acetate precipitation]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Cloning Cloning]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Cloning Cloning]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Dephosphorylation Dephosphorylation]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Dephosphorylation Dephosphorylation]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Ligation DNA Ligation]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/ DNA Ligation à la group transport]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Quantification_/_NanoDrop DNA Quantification / NanoDrop]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/DNA_Quantification_/_NanoDrop DNA Quantification / NanoDrop]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Gel_extraction Gel extraction]
 
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep Miniprep]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Miniprep Miniprep]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/mutagenic_PCR Mutagenic PCR]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/mutagenic_PCR Mutagenic PCR]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR PCR]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PCR PCR]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Primer_Design Primer Design]
 
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_DNA  Purification of DNA]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_DNA  Purification of DNA]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Restriction_digest Restriction digest]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Restriction_digest Restriction digest]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Resuspension_of_primers Resuspension of primers]
 
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Salting_Out Salting Out]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Salting_Out Salting Out]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Sequencing_DNA Sequencing DNA]
+
* [http://www.eurofinsdna.com/de/home.html?wmc=GA_DE-Corporate&gclid=CODAs8CZ1LICFQVf3god9AIAag Sequencing DNA]
-
=== [http://en.wikipedia.org/wiki/RNA RNA] ===
 
=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein Protein] ===
=== [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein Protein] ===
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Activity_assay Activity assay]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Activity_assay Activity assay]
Line 102: Line 93:
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_Periplasmatic_Proteins Purification of Periplasmatic Proteins]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_Periplasmatic_Proteins Purification of Periplasmatic Proteins]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SDS-Page SDS-Page]
-
 
-
== In silico ==
 
-
=== Data analysis ===
 
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Information_theory Information theory]
 
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/R_Programming R Programming]
 
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Mathlab_/_Simulink Mathlab / Simulink]
 
-
 
-
=== Databases ===
 
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PDB PDB]
 
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/CAS CAS]
 
-
 
-
=== Modelling ===
 
-
* [https://2012.igem.org/Team:TU_Darmstadt/Labjournal/Simulation/GNM Gaussian Network Modelling]
 
-
 
-
=== Sequence analysis ===
 
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/BLAST BLAST]
 
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SAGE SAGE]
 
-
 
-
=== Structure analysis ===
 
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/PDB PDB]
 
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Protein_structure_prediction Protein structure prediction]
 
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/RNA_secondary_structure_prediction RNA secondary structure prediction]
 
== Material Science ==
== Material Science ==
Line 131: Line 100:
== Miscellaneous ==
== Miscellaneous ==
-
=== Cell culture ===
+
=== Staining ===
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Pouring_LB_plates Pouring LB plates]
+
-
 
+
-
=== Microscopy ===
+
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Antibody_staining Antibody staining]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Antibody_staining Antibody staining]
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Determining_an_objects_size Determining an objects size]
+
 
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Preparing_slides Preparing slides]
+
=== Analytics ===
-
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Staining Staining]
+
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/TEM-EDX TEM-EDX Measurements]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/GC-MS GC-MS Measurements]
 +
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/SamplePreparationEDX Preparation of Escherichia Coli for the energy dispersive X-ray spectroscopy (EDX)]
=== ILDs / Improvised Lab Devices ===
=== ILDs / Improvised Lab Devices ===
Line 145: Line 113:
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Gel_pocket_size_reduction Gel pocket size reduction]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Gel_pocket_size_reduction Gel pocket size reduction]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Growing_iGEMlers Growing iGEMlers]
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Growing_iGEMlers Growing iGEMlers]
-
 
-
=== Analytics ===
 

Latest revision as of 21:45, 26 September 2012

Protocols

The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012. The corresponding materials can be found in the materials section.

Contents

In vivo

Escherichia coli

Comamonas testosteroni

In vitro

Nucleic acids

DNA

Protein

Material Science

Miscellaneous

Staining

Analytics

ILDs / Improvised Lab Devices