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Team:TU Darmstadt/Protocols
From 2012.igem.org
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* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Affinity_chromatography Affinity chromatography] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Affinity_chromatography Affinity chromatography] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/IMAC Immobilized metal ion affinity chromatography (IMAC)] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/IMAC Immobilized metal ion affinity chromatography (IMAC)] | ||
| + | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/pNP_Assay#Enzyme pNP-Assay Enzyme] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Proteinpurification Proteinpurification] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Proteinpurification Proteinpurification] | ||
* [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_Periplasmatic_Proteins Purification of Periplasmatic Proteins] | * [https://2012.igem.org/Team:TU_Darmstadt/Protocols/Purification_of_Periplasmatic_Proteins Purification of Periplasmatic Proteins] | ||
Revision as of 12:23, 24 September 2012
Protocols
The following page(s) gives an overview of the protocols and possible modifications that where used and proven working during the iGEM PET.erminators project 2012. The corresponding materials can be found in the materials section.
Contents |
In vivo
Escherichia coli
- Bacterial cell culture
- Bacterial transformation aka TRAFO
- Cell counting/plating
- Chemically competent cells
- Colony PCR
- Electrocompetent cells
- Electroporation
- Engineering BioBrick vectors from BioBrick parts/Colony PCR
- Glycerine Stocks
- Heat Shock Transformation
- In-fusion BioBrick assembly
- Miniprep / Fermentas kit
- Miniprep / Promega kit
- Miniprep / Qiagen kit
- Protein Expression Est13
- Protein Expression FsC
- Transformation of chemically competent cells
- Western blot
Comamonas testosteroni
In vitro
Nucleic acids
DNA
- Agarose gel electrophoresis
- Ammonium sulfate precipitation
- Cloning
- Dephosphorylation
- DNA Ligation
- DNA Quantification / NanoDrop
- Gel extraction
- Miniprep
- PCR
- Primer Design
- Purification of DNA
- Restriction digest
- Resuspension of primers
- Salting Out
- Sequencing DNA
RNA
Protein
- Activity assay
- Affinity chromatography
- Immobilized metal ion affinity chromatography (IMAC)
- pNP-Assay Enzyme
- Proteinpurification
- Purification of Periplasmatic Proteins
- SDS-Page
In silico
Data analysis
Databases
Modelling
Sequence analysis
Structure analysis
Material Science
- Synthesis of paranitrophenylesters with acyl chlorides in presence of triethylamine
- Synthesis of polyethylene terephthalate in presence of sulfuric acid
- Surface analysis of polyethylene terephthalate with atomic force microscopy
Miscellaneous
Cell culture
Microscopy
ILDs / Improvised Lab Devices
- x bp Primer Designer
- Enhancing a thermo cyclers cooling capabilites
- Gel pocket size reduction
- Growing iGEMlers
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