Team:TU Darmstadt/Materials

Materials

This page contains detailed instructions on the preparation of all employed materials including suppliers and working conditions. The pages structure is derived from the OWW materials page.

Can't find what you seek? Try their excellent page.

Strains

• DH5α
• TOP10

Media

Modified Media

• LB Bromthymol blue
• LB Tributyrin

Standard Media

• DYT
• LB
• SOB
• SOC

Antibiotics

• Ampicillin
• Chloramphenicol
• Kanamycin

Enzymes

Phosphatases

• Antarctic Phosphatase

DNA polymerases

• Phusion
• Taq polymerase

DNA ligases

• T4 DNA ligase

Restriction enzymes

• BsaI
• EcoRI
• PstI
• SpeI
• XbaI

Other

Proteases

Electrophoresis

Agarose gel electrophoresis

• Agarose gel loading buffer
• Bromphenol blue
• DNA ladder
• Ethidium Bromide
• Roti^® SafeGelStain
• TAE

PAGE

• Coomassie stain
• Destain
• SDS-Page

Buffers

• Alkaline phosphate buffer
• Blotting buffer
• NEB buffers
• PBS
• Quiagen buffers
• Resuspension buffer
• Tris

Acids and bases

• Imidazole

Other chemicals

• Arabinose
• BCIP
• EDTA
• NiCl₂
• NBT
• PET
• TPA

Reporters

Dyes and stains

• DAPI

Fluorescent proteins

• GFP

Enzymes

Plasmids

• BBa J61002
• pEST100
• pPR-IBA2
• pSB1A2
• pSB1C3
• pSB1AK3

Primers

• Ara RBS
• Primer Degradation
• tctA503 BioBrick
• tctA503 Pst1
• tctA505 BioBrick
• tctA505 Pst1
• tctB162 BioBrick
• tctB162 Pst1
• tctB162 RBS
• tctB197 BioBrick
• tctB197 RBS
• tphC BioBrick
• tphC Pst1

Detergents

Miscellaneous

• Equipment
• Materials

... 2 Uhr ich geh ins Bett... Du Faulpelz !!!!!!!!!!!!!!!!!!!!! Stimme zu! Einfach nur faul :D :D :D