Team:TMU-Tokyo

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Welcome to Team; TMU-Tokyo wiki!

Our project is Chef Ant E. coli, Escherichia coli transformeted formaldehyde detoxification function.
The first of formaldehyde detoxification pathway is conversion formaldehyde to formate, as the right image. Chef Ant E. coli was named after this stage, formate was first purified from ants and farther formate conversion is seemed like ant eater.






Project Abstract





Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials)
Also, since formaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. This year, in Japan, the detection of formaldehyde in Tone river became an issue.

We planed to create E. coli with an ability to detect and detoxify formaldehyde. named Chef Ant E. coli. About detection, we try to visualize formaldehyde by ligating regulated promoter, frmR and GFP. Moreover we plan to overexpress two enzymes in Chef Ant E. coli.
First, formaldehyde dehydrogenase decomposes formaldehyde to formate. The gene of formaldehyde dehydrogenase is from Pseudomonas Putida.
Second, formate dehydrogenase converts formate to CO₂ and H₂O. The gene of formate dehydrogenase is from Methylbacterium extorquens.

More information is available here.



Image of Chef Ant E.coli.