http://2012.igem.org/wiki/index.php?title=Team:Stanford-Brown/Biomining/Introduction&feed=atom&action=historyTeam:Stanford-Brown/Biomining/Introduction - Revision history2024-03-28T18:56:11ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Stanford-Brown/Biomining/Introduction&diff=263170&oldid=prevBbajar: /* Biomining */2012-10-04T00:58:17Z<p><span class="autocomment">Biomining</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 00:58, 4 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 21:</td>
<td colspan="2" class="diff-lineno">Line 21:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally, we redesigned Team Slovenia 2008's [http://partsregistry.org/wiki/index.php?title=Part:BBa_K133038 flagellar protein expression system], “FliC,” to have a general-use multiple cloning site. This way, anyone wishing to express proteins on flagella can do so using our [http://partsregistry.org/Part:BBa_K847101 biobrick].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally, we redesigned Team Slovenia 2008's [http://partsregistry.org/wiki/index.php?title=Part:BBa_K133038 flagellar protein expression system], “FliC,” to have a general-use multiple cloning site. This way, anyone wishing to express proteins on flagella can do so using our [http://partsregistry.org/Part:BBa_K847101 biobrick].</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[File:Biomining.png|<del class="diffchange diffchange-inline">left</del>|text-bottom|300px]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[File:Biomining.png|<ins class="diffchange diffchange-inline">right</ins>|text-bottom|300px]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''So what did we do?'''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''So what did we do?'''</div></td></tr>
</table>Bbajarhttp://2012.igem.org/wiki/index.php?title=Team:Stanford-Brown/Biomining/Introduction&diff=263165&oldid=prevBbajar: /* Biomining */2012-10-04T00:57:59Z<p><span class="autocomment">Biomining</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 00:57, 4 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 22:</td>
<td colspan="2" class="diff-lineno">Line 22:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:Biomining.png|left|text-bottom|300px]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:Biomining.png|left|text-bottom|300px]]</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">'''So what did we do?'''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*We created a standard for expression of proteins on flagellin protein FliC in part K847101.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">*We engineered K847101 to express metalbinding ions to harvest metal ions ''in situ''.</ins></div></td></tr>
</table>Bbajarhttp://2012.igem.org/wiki/index.php?title=Team:Stanford-Brown/Biomining/Introduction&diff=263140&oldid=prevBbajar: /* Biomining */2012-10-04T00:56:41Z<p><span class="autocomment">Biomining</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 00:56, 4 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 16:</td>
<td colspan="2" class="diff-lineno">Line 16:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Biomining''' ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Biomining''' ==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The ability to extract or recycle metals is key to long-term human survival in space. Flying up traditional heavy-duty equipment to perform these functions is not viable economically or structurally. Bacteria are small, easy to transport, and can multiply and regenerate themselves. They can be used to extract metals from sediment on solid planets or asteroids, or to recycle metal electronics aboard spacecraft.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The ability to extract or recycle metals is key to long-term human survival in space. Flying up traditional heavy-duty equipment to perform these functions is not viable economically or structurally. Bacteria are small, easy to transport, and can multiply and regenerate themselves. They can be used to extract metals from sediment on solid planets or asteroids, or to recycle metal electronics aboard spacecraft.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Existing bacteria with the ability to mine metal gather metal ions either within the cell body or on the cell surface. For our project, we modified a unique flagellar protein expression system, originally employed by [https://2008.igem.org/Team:Slovenia iGEM Team Slovenia 2008] as a novel flagellin vaccine. We attempted to make metal ion harvesting more robust by expressing metal binding peptides on bacterial flagella: we could chemically remove flagella from the bacterial body and collect the ions without killing the organism. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Existing bacteria with the ability to mine metal gather metal ions either within the cell body or on the cell surface. For our project, we modified a unique flagellar protein expression system, originally employed by [https://2008.igem.org/Team:Slovenia iGEM Team Slovenia 2008] as a novel flagellin vaccine. We attempted to make metal ion harvesting more robust by expressing metal binding peptides on bacterial flagella: we could chemically remove flagella from the bacterial body and collect the ions without killing the organism. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Additionally, we redesigned Team Slovenia 2008's [http://partsregistry.org/wiki/index.php?title=Part:BBa_K133038 flagellar protein expression system], “FliC,” to have a general-use multiple cloning site. This way, anyone wishing to express proteins on flagella can do so using our [http://partsregistry.org/Part:BBa_K847101 biobrick].</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">Additionally, we redesigned Team Slovenia 2008's </del>[<del class="diffchange diffchange-inline">http</del>:<del class="diffchange diffchange-inline">//partsregistry</del>.<del class="diffchange diffchange-inline">org/wiki/index.php?title=Part:BBa_K133038 flagellar protein expression system], “FliC,” to have a general</del>-<del class="diffchange diffchange-inline">use multiple cloning site. This way, anyone wishing to express proteins on flagella can do so using our [http://partsregistry.org/Part:BBa_K847101 biobrick</del>]<del class="diffchange diffchange-inline">.</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[<ins class="diffchange diffchange-inline">[File</ins>:<ins class="diffchange diffchange-inline">Biomining</ins>.<ins class="diffchange diffchange-inline">png|left|text</ins>-<ins class="diffchange diffchange-inline">bottom|300px]</ins>]</div></td></tr>
</table>Bbajarhttp://2012.igem.org/wiki/index.php?title=Team:Stanford-Brown/Biomining/Introduction&diff=256908&oldid=prevBbajar at 08:53, 3 October 20122012-10-03T08:53:36Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 08:53, 3 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 7:</td>
<td colspan="2" class="diff-lineno">Line 7:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="/Team:Stanford-Brown/Biomining/Harvesting">Harvesting</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="/Team:Stanford-Brown/Biomining/Harvesting">Harvesting</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="/Team:Stanford-Brown/Parts">BioBricks</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="/Team:Stanford-Brown/Parts">BioBricks</a></li></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><li><a href="https://docs.google.com/a/stanford.edu/document/d/1RfsxBERl_GZoXgjWbqzlezqknuxjKNX8bjLudxNzz9U/edit">Notebook</a></li></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><li><a href="/Team:Stanford-Brown/Protocols">Protocols</a></li></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
</table>Bbajarhttp://2012.igem.org/wiki/index.php?title=Team:Stanford-Brown/Biomining/Introduction&diff=253234&oldid=prevJsborden: /* Biomining */2012-10-03T02:22:31Z<p><span class="autocomment">Biomining</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:22, 3 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 15:</td>
<td colspan="2" class="diff-lineno">Line 15:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The ability to extract or recycle metals is key to long-term human survival in space. Flying up traditional heavy-duty equipment to perform these functions is not viable economically or structurally. Bacteria are small, easy to transport, and can multiply and regenerate themselves. They can be used to extract metals from sediment on solid planets or asteroids, or to recycle metal electronics aboard spacecraft.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The ability to extract or recycle metals is key to long-term human survival in space. Flying up traditional heavy-duty equipment to perform these functions is not viable economically or structurally. Bacteria are small, easy to transport, and can multiply and regenerate themselves. They can be used to extract metals from sediment on solid planets or asteroids, or to recycle metal electronics aboard spacecraft.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Existing bacteria with the ability to mine metal gather metal ions either within the cell body or on the cell surface. For our project, we modified a unique flagellar protein expression system, originally employed by [https://2008.igem.org/Team:Slovenia iGEM Team Slovenia 2008] as a novel flagellin vaccine. We attempted to make metal ion harvesting more robust by expressing metal binding peptides on bacterial flagella: we could chemically remove flagella from the bacterial body and collect the ions without killing the organism. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Existing bacteria with the ability to mine metal gather metal ions either within the cell body or on the cell surface. For our project, we modified a unique flagellar protein expression system, originally employed by [https://2008.igem.org/Team:Slovenia iGEM Team Slovenia 2008] as a novel flagellin vaccine. We attempted to make metal ion harvesting more robust by expressing metal binding peptides on bacterial flagella: we could chemically remove flagella from the bacterial body and collect the ions without killing the organism. </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally, we redesigned Team Slovenia 2008's [http://partsregistry.org/wiki/index.php?title=Part:BBa_K133038 flagellar protein expression system], “FliC,” to have a general-use multiple cloning site. This way, anyone wishing to express proteins on flagella can do so using our [http://partsregistry.org/Part:BBa_K847101 biobrick].</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally, we redesigned Team Slovenia 2008's [http://partsregistry.org/wiki/index.php?title=Part:BBa_K133038 flagellar protein expression system], “FliC,” to have a general-use multiple cloning site. This way, anyone wishing to express proteins on flagella can do so using our [http://partsregistry.org/Part:BBa_K847101 biobrick].</div></td></tr>
</table>Jsbordenhttp://2012.igem.org/wiki/index.php?title=Team:Stanford-Brown/Biomining/Introduction&diff=252797&oldid=prevBbajar at 01:14, 3 October 20122012-10-03T01:14:04Z<p></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 01:14, 3 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 6:</td>
<td colspan="2" class="diff-lineno">Line 6:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li id="active"><a href="#" id="current">Introduction</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li id="active"><a href="#" id="current">Introduction</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="/Team:Stanford-Brown/Biomining/Harvesting">Harvesting</a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><li><a href="/Team:Stanford-Brown/Biomining/Harvesting">Harvesting</a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><li><a href="/Team:Stanford-Brown/<del class="diffchange diffchange-inline">Biomining/BioBricks</del>">BioBricks</a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><li><a href="/Team:Stanford-Brown/<ins class="diffchange diffchange-inline">Parts</ins>">BioBricks</a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div></div></div></td></tr>
</table>Bbajarhttp://2012.igem.org/wiki/index.php?title=Team:Stanford-Brown/Biomining/Introduction&diff=252487&oldid=prevMichelleyu: /* Biomining */2012-10-03T00:28:33Z<p><span class="autocomment">Biomining</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 00:28, 3 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 17:</td>
<td colspan="2" class="diff-lineno">Line 17:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Existing bacteria with the ability to mine metal gather metal ions either within the cell body or on the cell surface. For our project, we modified a unique flagellar protein expression system, originally employed by [https://2008.igem.org/Team:Slovenia iGEM Team Slovenia 2008] as a novel flagellin vaccine. We attempted to make metal ion harvesting more robust by expressing metal binding peptides on bacterial flagella: we could chemically remove flagella from the bacterial body and collect the ions without killing the organism. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Existing bacteria with the ability to mine metal gather metal ions either within the cell body or on the cell surface. For our project, we modified a unique flagellar protein expression system, originally employed by [https://2008.igem.org/Team:Slovenia iGEM Team Slovenia 2008] as a novel flagellin vaccine. We attempted to make metal ion harvesting more robust by expressing metal binding peptides on bacterial flagella: we could chemically remove flagella from the bacterial body and collect the ions without killing the organism. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Additionally, we redesigned Team Slovenia 2008's flagellar protein expression system, “FliC,” to have a general-use multiple cloning site. This way, anyone wishing to express proteins on flagella can do so using our [http://partsregistry.org/Part:BBa_K847101 biobrick].</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Additionally, we redesigned Team Slovenia 2008's <ins class="diffchange diffchange-inline">[http://partsregistry.org/wiki/index.php?title=Part:BBa_K133038 </ins>flagellar protein expression system<ins class="diffchange diffchange-inline">]</ins>, “FliC,” to have a general-use multiple cloning site. This way, anyone wishing to express proteins on flagella can do so using our [http://partsregistry.org/Part:BBa_K847101 biobrick].</div></td></tr>
</table>Michelleyuhttp://2012.igem.org/wiki/index.php?title=Team:Stanford-Brown/Biomining/Introduction&diff=252455&oldid=prevMichelleyu: /* Biomining */2012-10-03T00:21:50Z<p><span class="autocomment">Biomining</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 00:21, 3 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 17:</td>
<td colspan="2" class="diff-lineno">Line 17:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Existing bacteria with the ability to mine metal gather metal ions either within the cell body or on the cell surface. For our project, we modified a unique flagellar protein expression system, originally employed by [https://2008.igem.org/Team:Slovenia iGEM Team Slovenia 2008] as a novel flagellin vaccine. We attempted to make metal ion harvesting more robust by expressing metal binding peptides on bacterial flagella: we could chemically remove flagella from the bacterial body and collect the ions without killing the organism. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Existing bacteria with the ability to mine metal gather metal ions either within the cell body or on the cell surface. For our project, we modified a unique flagellar protein expression system, originally employed by [https://2008.igem.org/Team:Slovenia iGEM Team Slovenia 2008] as a novel flagellin vaccine. We attempted to make metal ion harvesting more robust by expressing metal binding peptides on bacterial flagella: we could chemically remove flagella from the bacterial body and collect the ions without killing the organism. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Additionally, we redesigned Team Slovenia 2008's flagellar protein expression system, “FliC,” to have a general-use multiple cloning site. This way, anyone wishing to express proteins on flagella can do so using our <del class="diffchange diffchange-inline"><a href="</del>http://partsregistry.org/Part:BBa_K847101<del class="diffchange diffchange-inline">"></del>biobrick<del class="diffchange diffchange-inline"></a></del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Additionally, we redesigned Team Slovenia 2008's flagellar protein expression system, “FliC,” to have a general-use multiple cloning site. This way, anyone wishing to express proteins on flagella can do so using our <ins class="diffchange diffchange-inline">[</ins>http://partsregistry.org/Part:BBa_K847101 biobrick<ins class="diffchange diffchange-inline">]</ins>.</div></td></tr>
</table>Michelleyuhttp://2012.igem.org/wiki/index.php?title=Team:Stanford-Brown/Biomining/Introduction&diff=252453&oldid=prevMichelleyu: /* Biomining */2012-10-03T00:21:24Z<p><span class="autocomment">Biomining</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 00:21, 3 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 15:</td>
<td colspan="2" class="diff-lineno">Line 15:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The ability to extract or recycle metals is key to long-term human survival in space. Flying up traditional heavy-duty equipment to perform these functions is not viable economically or structurally. Bacteria are small, easy to transport, and can multiply and regenerate themselves. They can be used to extract metals from sediment on solid planets or asteroids, or to recycle metal electronics aboard spacecraft.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The ability to extract or recycle metals is key to long-term human survival in space. Flying up traditional heavy-duty equipment to perform these functions is not viable economically or structurally. Bacteria are small, easy to transport, and can multiply and regenerate themselves. They can be used to extract metals from sediment on solid planets or asteroids, or to recycle metal electronics aboard spacecraft.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Existing bacteria with the ability to mine metal gather metal ions either within the cell body or on the cell surface. For our project, we modified a unique flagellar protein expression system, originally employed by <del class="diffchange diffchange-inline"><a href="</del>https://2008.igem.org/Team:Slovenia<del class="diffchange diffchange-inline">"></del>iGEM Team Slovenia 2008<del class="diffchange diffchange-inline"></a> </del>as a novel flagellin vaccine. We attempted to make metal ion harvesting more robust by expressing metal binding peptides on bacterial flagella: we could chemically remove flagella from the bacterial body and collect the ions without killing the organism. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Existing bacteria with the ability to mine metal gather metal ions either within the cell body or on the cell surface. For our project, we modified a unique flagellar protein expression system, originally employed by <ins class="diffchange diffchange-inline">[</ins>https://2008.igem.org/Team:Slovenia iGEM Team Slovenia 2008<ins class="diffchange diffchange-inline">] </ins>as a novel flagellin vaccine. We attempted to make metal ion harvesting more robust by expressing metal binding peptides on bacterial flagella: we could chemically remove flagella from the bacterial body and collect the ions without killing the organism. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally, we redesigned Team Slovenia 2008's flagellar protein expression system, “FliC,” to have a general-use multiple cloning site. This way, anyone wishing to express proteins on flagella can do so using our <a href="http://partsregistry.org/Part:BBa_K847101">biobrick</a>.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Additionally, we redesigned Team Slovenia 2008's flagellar protein expression system, “FliC,” to have a general-use multiple cloning site. This way, anyone wishing to express proteins on flagella can do so using our <a href="http://partsregistry.org/Part:BBa_K847101">biobrick</a>.</div></td></tr>
</table>Michelleyuhttp://2012.igem.org/wiki/index.php?title=Team:Stanford-Brown/Biomining/Introduction&diff=252444&oldid=prevMichelleyu: /* Biomining */2012-10-03T00:19:57Z<p><span class="autocomment">Biomining</span></p>
<table style="background-color: white; color:black;">
<col class='diff-marker' />
<col class='diff-content' />
<col class='diff-marker' />
<col class='diff-content' />
<tr valign='top'>
<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 00:19, 3 October 2012</td>
</tr><tr><td colspan="2" class="diff-lineno">Line 15:</td>
<td colspan="2" class="diff-lineno">Line 15:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The ability to extract or recycle metals is key to long-term human survival in space. Flying up traditional heavy-duty equipment to perform these functions is not viable economically or structurally. Bacteria are small, easy to transport, and can multiply and regenerate themselves. They can be used to extract metals from sediment on solid planets or asteroids, or to recycle metal electronics aboard spacecraft.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The ability to extract or recycle metals is key to long-term human survival in space. Flying up traditional heavy-duty equipment to perform these functions is not viable economically or structurally. Bacteria are small, easy to transport, and can multiply and regenerate themselves. They can be used to extract metals from sediment on solid planets or asteroids, or to recycle metal electronics aboard spacecraft.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Existing bacteria with the ability to mine metal gather metal ions either within the cell body or on the cell surface. For our project, we modified a unique flagellar protein expression system, originally employed by iGEM Team Slovenia 2008 as a novel flagellin vaccine. We attempted to make metal ion harvesting more robust by expressing metal binding peptides on bacterial flagella: we could chemically remove flagella from the bacterial body and collect the ions without killing the organism. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Existing bacteria with the ability to mine metal gather metal ions either within the cell body or on the cell surface. For our project, we modified a unique flagellar protein expression system, originally employed by <ins class="diffchange diffchange-inline"><a href="https://2008.igem.org/Team:Slovenia"></ins>iGEM Team Slovenia 2008<ins class="diffchange diffchange-inline"></a> </ins>as a novel flagellin vaccine. We attempted to make metal ion harvesting more robust by expressing metal binding peptides on bacterial flagella: we could chemically remove flagella from the bacterial body and collect the ions without killing the organism. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Additionally, we redesigned Team Slovenia 2008's flagellar protein expression system, “FliC,” to have a general-use multiple cloning site. This way, anyone wishing to express proteins on flagella can do so using our biobrick.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Additionally, we redesigned Team Slovenia 2008's flagellar protein expression system, “FliC,” to have a general-use multiple cloning site. This way, anyone wishing to express proteins on flagella can do so using our <ins class="diffchange diffchange-inline"><a href="http://partsregistry.org/Part:BBa_K847101"></ins>biobrick<ins class="diffchange diffchange-inline"></a></ins>.</div></td></tr>
</table>Michelleyu