Team:Slovenia/SimulationsTest

From 2012.igem.org

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<html>
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<script type="text/javascript" src="http://igem2012.fri.uni-lj.si/scripts/inputcheck.js"></script>
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 +
<body>
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    <form name="form1" action="http://igem2012.fri.uni-lj.si:443/cellulator/bistable" target="_blank" method="GET">
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      <input type="hidden" name="check_submit" value="1"/>
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      <hr />
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      <b>INPUT VECTORS DOSAGE</b> <br/>
 +
     
 +
      <table>
 +
        <th>
 +
        <tr><td>Construct</td><td>Dosage</td></tr>
 +
        </th>
 +
      <tr><td><strong>[A]_pCMV_TAL-B:KRAB_BFP</strong> </td><td> <input type="text" name="plasmid1" size="5" value="190" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
 +
      <tr><td><strong>[B]_pCMV_TAL-A:KRAB_mCitrine</strong> </td><td> <input type="text" name="plasmid2" size="5" value="180" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
 +
      <tr><td><strong>pCMV_[PIR]_TAL-B:KRAB</strong> </td><td> <input type="text" name="plasmid3" size="5" value="190" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
 +
      <tr><td><strong>pCMV_[ETR]_TAL-B:KRAB</strong> </td><td> <input type="text" name="plasmid4" size="5" value="180" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
 +
      <tr><td><strong>pCMV_PIP:KRAB</strong> </td><td> <input type="text" name="plasmid5" size="5" value="180" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
 +
      <tr><td><strong>pCMV_E:KRAB </strong> </td><td> <input type="text" name="plasmid6" size="5" value="180" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
 +
      </table>
 +
      <font color="red">(Enter dosages for corresponding plasmids.)</font><br /><br />
 +
     
 +
     
 +
     
 +
      <hr/>
 +
      <b>TRANSCRIPTION FACTORS</b>
 +
      <br />
 +
      <input type="checkbox" name="req" value="1" /> Equal transcription factors
 +
      <br/>
 +
      <font color="red">(Transcription factors will alter transcription with equal rates)</font><br /> <br />
 +
     
 +
      <hr/>
 +
      <b>INDUCERS</b> <br/>
 +
     
 +
      <table>
 +
      <th><tr><td><i>Pristinamycin</i></td><td></td></tr></th>
 +
      <tr>
 +
        <td>Input times [min]</td>
 +
        <td><input type="text" name="pc_start1" size="5" value="2000" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
 +
        <td><input type="text" name="pc_start2" size="5" value="10000" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
 +
        <td><input type="text" name="pc_start3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
 +
        <td><input type="text" name="pc_start4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
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        <td><input type="text" name="pc_start5" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
 +
      </tr>
 +
      <tr>
 +
        <td>Durations [min]</td>
 +
        <td><input type="text" name="pc_duration1" size="5" value="1000" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
 +
        <td><input type="text" name="pc_duration2" size="5" value="1000" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
 +
        <td><input type="text" name="pc_duration3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
 +
        <td><input type="text" name="pc_duration4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
 +
        <td><input type="text" name="pc_duration5" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
 +
      </tr>
 +
      </table>
 +
 
 +
      <br/>
 +
 +
      <table>
 +
      <th><tr><td><i>Erithromycin</i></td><td></td></tr></th>
 +
      <tr>
 +
        <td>Input times [min]</td>
 +
        <td><input type="text" name="rg_start1" size="5" value="6000" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
 +
        <td><input type="text" name="rg_start2" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
 +
        <td><input type="text" name="rg_start3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
 +
        <td><input type="text" name="rg_start4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
 +
        <td><input type="text" name="rg_start5" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
 +
      </tr>
 +
      <tr>
 +
        <td>Durations [min]</td>
 +
        <td><input type="text" name="rg_duration1" size="5" value="1000" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
 +
        <td><input type="text" name="rg_duration2" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
 +
        <td><input type="text" name="rg_duration3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
 +
        <td><input type="text" name="rg_duration4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
 +
        <td><input type="text" name="rg_duration5" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
 +
      </tr>
 +
      </table>
 +
     
 +
      <font color="red">(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )</font><br />
 +
 +
      <br />
 +
      <b>SIMULATION</b> <br/>
 +
 +
 +
      <hr />
 +
      Time: <input type="text" name="time" size="5" value="15000" onKeyPress="return numbersonly(this, event)" maxlength="7"/> min<br />
 +
      <font color="red">(Simulation running time)</font><br /><br />
 +
 +
      <input type="submit" value="SIMULATE" /> <br /><br /><br />
 +
    </form>
 +
  </body>
 +
</html>
 +
 +
<html>
 +
 +
<SCRIPT TYPE="text/javascript" src="http://igem2012.fri.uni-lj.si/scripts/inputcheck.js"></script>
 +
 +
 +
<body>
 +
 +
<hr />
 +
 +
 +
    <form name="form1" action="http://igem2012.fri.uni-lj.si:443/cellulator/bistable_feedback" target="_blank" method="GET">
 +
      <input type="hidden" name="check_submit" value="1"/>
 +
      <b>INPUT VECTORS DOSAGE</b>
 +
     
 +
      <table>
 +
        <th>
 +
        <tr><td>Construct</td><td>Dosage</td></tr>
 +
        </th>
 +
        <tr><td><strong>[A]_pCMV_TAL-B:KRAB_TAL-A:VP16_BFP </strong></td><td><input type="text" name="plasmid1" size="5" value="200" onKeyPress="return numbersonly(this, event)"/> ng</td></tr>
 +
        <tr><td><strong>[B]_pCMV_TAL-A:KRAB_TAL-B:VP16_mCitrine</strong></td><td><input type="text" name="plasmid2" size="5" value="200" onKeyPress="return numbersonly(this, event)"/> ng</td></tr>
 +
        <tr><td><strong>pCMV_[PIR]_TAL-B:KRAB_TAL-A:VP16</strong></td><td><input type="text" name="plasmid3" size="5" value="200" onKeyPress="return numbersonly(this, event)"/> ng</td></tr>
 +
        <tr><td><strong>pCMV_[ETR]_TAL-A:KRAB_TAL-B:VP16</strong></td><td><input type="text" name="plasmid4" size="5" value="200" onKeyPress="return numbersonly(this, event)"/> ng</td></tr>
 +
        <tr><td><strong>pCMV_PIP:KRAB_E:KRAB</strong></td><td><input type="text" name="plasmid5" size="5" value="200" onKeyPress="return numbersonly(this, event)"/> ng</td></tr>
 +
      </table>
 +
      <font color="red">(Enter dosages for corresponding plasmids.)</font><br /><br />
 +
     
 +
      <hr/>
 +
      <b>TRANSCRIPTION FACTORS</b>
 +
      <br />
 +
      <input type="checkbox" name="req" value="1" /> Equal transcription factors <br />
 +
      <font color="red">(Transcription factors will alter transcription with equal rates)</font><br /><br />
 +
      <hr/>
 +
      <b>INDUCERS</b> <br/>
 +
 +
     
 +
      <table>
 +
      <th><tr><td><i>Pristinamycin</i></td><td></td></tr></th>
 +
      <tr>
 +
        <td>Input times [min]</td>
 +
        <td><input type="text" name="pc_start1" size="5" value="7000" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
 +
        <td><input type="text" name="pc_start2" size="5" value="1000" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
 +
        <td><input type="text" name="pc_start3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
 +
        <td><input type="text" name="pc_start4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
 +
        <td><input type="text" name="pc_start5" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
 +
      </tr>
 +
      <tr>
 +
        <td>Durations [min]</td>
 +
        <td><input type="text" name="pc_duration1" size="5" value="1000" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
 +
        <td><input type="text" name="pc_duration2" size="5" value="1000" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
 +
        <td><input type="text" name="pc_duration3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
 +
        <td><input type="text" name="pc_duration4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
 +
        <td><input type="text" name="pc_duration5" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"><br/></td>
 +
      </tr>
 +
      </table>
 +
 
 +
      <br/>
 +
 +
      <table>
 +
      <th><tr><td><i>Erithromycin</i></td><td></td></tr></th>
 +
      <tr>
 +
        <td>Input times [min]</td>
 +
        <td><input type="text" name="rm_start1" size="5" value="3000" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
 +
        <td><input type="text" name="rm_start2" size="5" value="14000" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
 +
        <td><input type="text" name="rm_start3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
 +
        <td><input type="text" name="rm_start4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
 +
        <td><input type="text" name="rm_start5" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"/></td>
 +
      </tr>
 +
      <tr>
 +
        <td>Durations [min]</td>
 +
        <td><input type="text" name="rm_duration1" size="5" value="1000" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
 +
        <td><input type="text" name="rm_duration2" size="5" value="2000" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
 +
        <td><input type="text" name="rm_duration3" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
 +
        <td><input type="text" name="rm_duration4" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
 +
        <td><input type="text" name="rm_duration5" size="5" value="" onKeyPress="return numbersonly(this, event)" maxlength="6"></td>
 +
      </tr>
 +
      </table>
 +
      <font color="red">(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )</font>
 +
      <br />
 +
      <br />
 +
     
 +
 +
 +
 +
      <hr/>
 +
      <b>SIMULATION</b> <br/>
 +
      Time: <input type="text" name="time" size="5" value="20000"/> min<br />
 +
      <font color="red">(Simulation running time)</font><br />
 +
      <br />
 +
     
 +
      <input type="submit" value="SIMULATE" />
 +
    </form>
 +
  </body>
 +
</html>
 +
 +
 +
<!--
 +
== Tristable switch (2 inputs) ==
 +
== Tristable switch with positive feedback (2 inputs) ==
 +
-->

Latest revision as of 22:01, 22 September 2012

INPUT VECTORS DOSAGE
ConstructDosage
[A]_pCMV_TAL-B:KRAB_BFP ng
[B]_pCMV_TAL-A:KRAB_mCitrine ng
pCMV_[PIR]_TAL-B:KRAB ng
pCMV_[ETR]_TAL-B:KRAB ng
pCMV_PIP:KRAB ng
pCMV_E:KRAB ng
(Enter dosages for corresponding plasmids.)

TRANSCRIPTION FACTORS
Equal transcription factors
(Transcription factors will alter transcription with equal rates)

INDUCERS
Pristinamycin
Input times [min]
Durations [min]





Erithromycin
Input times [min]
Durations [min]
(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )

SIMULATION
Time: min
(Simulation running time)




INPUT VECTORS DOSAGE
ConstructDosage
[A]_pCMV_TAL-B:KRAB_TAL-A:VP16_BFP ng
[B]_pCMV_TAL-A:KRAB_TAL-B:VP16_mCitrine ng
pCMV_[PIR]_TAL-B:KRAB_TAL-A:VP16 ng
pCMV_[ETR]_TAL-A:KRAB_TAL-B:VP16 ng
pCMV_PIP:KRAB_E:KRAB ng
(Enter dosages for corresponding plasmids.)

TRANSCRIPTION FACTORS
Equal transcription factors
(Transcription factors will alter transcription with equal rates)

INDUCERS
Pristinamycin
Input times [min]
Durations [min]





Erithromycin
Input times [min]
Durations [min]
(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )

SIMULATION
Time: min
(Simulation running time)


Retrieved from "http://2012.igem.org/Team:Slovenia/SimulationsTest"