http://2012.igem.org/wiki/index.php?title=Team:Slovenia/SafetyMechanismsEscapeTag&feed=atom&action=historyTeam:Slovenia/SafetyMechanismsEscapeTag - Revision history2024-03-28T08:18:15ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Slovenia/SafetyMechanismsEscapeTag&diff=289632&oldid=prevStrazkosann at 20:59, 26 October 20122012-10-26T20:59:16Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 20:59, 26 October 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/TheSwitchDesignedTALregulators'><span>Designed TAL regulators</span></a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/TheSwitchDesignedTALregulators'><span>Designed TAL regulators</span></a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/TheSwitchMutualRepressorSwitch'><span>Mutual repressor switch</span></a></li> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/TheSwitchMutualRepressorSwitch'><span>Mutual repressor switch</span></a></li> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/TheSwitchPositiveFeedbackLoopSwitch'><table class="newtable"><tr class="newtable"><td class="newtable"><span>Positive feedback loop switch</span></td><td class="newtable"><img style="margin-right:-15px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/TheSwitchPositiveFeedbackLoopSwitch'><table <ins class="diffchange diffchange-inline">onclick="window.location = 'https://2012.igem.org/Team:Slovenia/TheSwitchPositiveFeedbackLoopSwitch';" </ins>class="newtable"><tr class="newtable"><td class="newtable"><span>Positive feedback loop switch</span></td><td class="newtable"><img style="margin-right:-15px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/TheSwitchControls'><table class="newtable"><tr class="newtable"><td class="newtable"><span>Controls</span></td><td class="newtable"><img style="margin-right:-81px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/TheSwitchControls'><table <ins class="diffchange diffchange-inline">onclick="window.location = 'https://2012.igem.org/Team:Slovenia/TheSwitchControls';" </ins>class="newtable"><tr class="newtable"><td class="newtable"><span>Controls</span></td><td class="newtable"><img style="margin-right:-81px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li> </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/SafetyMechanismsEscapeTag'><span>Escape tag</span></a></li> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/SafetyMechanismsEscapeTag'><span>Escape tag</span></a></li> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/SafetyMechanismsTermination'><span>Termination</span></a></li> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/SafetyMechanismsTermination'><span>Termination</span></a></li> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href=<del class="diffchange diffchange-inline">'</del>https://2012.igem.org/Team:Slovenia/SafetyMechanismsMicrocapsuleDegradation<del class="diffchange diffchange-inline">'</del>><table class="newtable"><tr class="newtable"><td class="newtable"><span>Microcapsule degradation</span></td><td class="newtable"><img style="margin-right:-15px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href=<ins class="diffchange diffchange-inline">"</ins>https://2012.igem.org/Team:Slovenia/SafetyMechanismsMicrocapsuleDegradation<ins class="diffchange diffchange-inline">"</ins>><table <ins class="diffchange diffchange-inline"> onclick="window.location = 'https://2012.igem.org/Team:Slovenia/SafetyMechanismsMicrocapsuleDegradation';" </ins>class="newtable"><tr class="newtable"><td class="newtable"><span>Microcapsule degradation</span></td><td class="newtable"><img style="margin-right:-15px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ImplementationHepatitisC'><span>Hepatitis C</span></a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ImplementationHepatitisC'><span>Hepatitis C</span></a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ImplementationIschaemicHeartDisease'><span>Ischaemic heart disease</span></a></li> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ImplementationIschaemicHeartDisease'><span>Ischaemic heart disease</span></a></li> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ImplementationImpact'><table class="newtable"><tr class="newtable"><td class="newtable"><span>Impact</span></td><td class="newtable"><img style="margin-right:-86px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ImplementationImpact'><table <ins class="diffchange diffchange-inline">onclick="window.location = 'https://2012.igem.org/Team:Slovenia/ImplementationImpact';" </ins>class="newtable"><tr class="newtable"><td class="newtable"><span>Impact</span></td><td class="newtable"><img style="margin-right:-86px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li> </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/Modeling'><span>Overview</span></a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/Modeling'><span>Overview</span></a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingPK'><table class="newtable"><tr class="newtable"><td class="newtable"><span>Pharmacokinetics</span></td><td class="newtable"><img style="margin-right:-15px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingPK'><table <ins class="diffchange diffchange-inline">onclick="window.location = 'https://2012.igem.org/Team:Slovenia/ModelingPK';" </ins>class="newtable"><tr class="newtable"><td class="newtable"><span>Pharmacokinetics</span></td><td class="newtable"><img style="margin-right:-15px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingMethods'><span>Modeling methods</span></a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingMethods'><span>Modeling methods</span></a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingMutualRepressorSwitch'><span>Mutual repressor switch</span></a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingMutualRepressorSwitch'><span>Mutual repressor switch</span></a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingPositiveFeedbackLoopSwitch'><span>Positive feedback loop switch</span></a></li> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingPositiveFeedbackLoopSwitch'><span>Positive feedback loop switch</span></a></li> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingQuantitativeModel'><table class="newtable"><tr class="newtable"><td class="newtable"><span>Experimental model</span></td><td class="newtable"><img style="margin-right:-15px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingQuantitativeModel'><table <ins class="diffchange diffchange-inline">onclick="window.location = 'https://2012.igem.org/Team:Slovenia/ModelingQuantitativeModel';" </ins>class="newtable"><tr class="newtable"><td class="newtable"><span>Experimental model</span></td><td class="newtable"><img style="margin-right:-15px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingInteractiveSimulations'><table class="newtable"><tr class="newtable"><td class="newtable"><span>Interactive simulations</span></td><td class="newtable"><img style="margin-right:-15px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingInteractiveSimulations'><table <ins class="diffchange diffchange-inline">onclick="window.location = 'https://2012.igem.org/Team:Slovenia/ModelingInteractiveSimulations';" </ins>class="newtable"><tr class="newtable"><td class="newtable"><span>Interactive simulations</span></td><td class="newtable"><img style="margin-right:-15px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/Notebook'><span>Experimental methods</span></a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/Notebook'><span>Experimental methods</span></a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/NotebookLablog'><table class="newtable"><tr class="newtable"><td class="newtable"><span>Lablog</span></td><td class="newtable"><img style="margin-right:-90px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/NotebookLablog'><table <ins class="diffchange diffchange-inline">onclick="window.location = 'https://2012.igem.org/Team:Slovenia/NotebookLablog';" </ins>class="newtable"><tr class="newtable"><td class="newtable"><span>Lablog</span></td><td class="newtable"><img style="margin-right:-90px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/NotebookLabSafety'><span>Lab safety</span></a></li> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/NotebookLabSafety'><span>Lab safety</span></a></li> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td></tr>
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</table>Strazkosannhttp://2012.igem.org/wiki/index.php?title=Team:Slovenia/SafetyMechanismsEscapeTag&diff=283683&oldid=prevMiha Jerala at 12:12, 26 October 20122012-10-26T12:12:19Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> their dissemination through the body we designed a <b>safety mechanism</b> that would <b>enhance</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> their dissemination through the body we designed a <b>safety mechanism</b> that would <b>enhance</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> the recognition and destruction</b> of the escaped therapeutic cells by the innate immune system.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> the recognition and destruction</b> of the escaped therapeutic cells by the innate immune system.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>We introduced an escape tag that labels the cells with a surface protein that alerts natural killer cells of the host organism to recognize and destroy cells.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>We introduced an escape tag that labels the cells with <ins class="diffchange diffchange-inline">MICA, </ins>a surface protein that alerts natural killer cells of the host organism to recognize and destroy cells.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>HEK293 cells expressing MICA protein were efficiently killed by human NK cells.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>HEK293 cells expressing MICA protein <ins class="diffchange diffchange-inline">on the surface </ins>were efficiently killed by human NK cells.</p></div></td></tr>
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</table>Miha Jeralahttp://2012.igem.org/wiki/index.php?title=Team:Slovenia/SafetyMechanismsEscapeTag&diff=280020&oldid=prevDusanv at 22:02, 25 October 20122012-10-25T22:02:31Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <li><a href='https://2012.igem.org/Team:Slovenia/TeamCollaborations'><table class="newtable"><tr class="newtable"><td class="newtable"><span>Collaborations</span></td><td class="newtable"><img style="margin-right:-20px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li></ins></div></td></tr>
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</table>Dusanvhttp://2012.igem.org/wiki/index.php?title=Team:Slovenia/SafetyMechanismsEscapeTag&diff=279051&oldid=prevStrazkosann at 17:16, 25 October 20122012-10-25T17:16:34Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><a style="position:absolute; top:0px; left:490px;" href="https://2012.igem.org/Main_Page"><b>iGEM 2012</b></a></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/TheSwitchDesignedTALregulators'><span>Designed TAL regulators</span></a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/TheSwitchDesignedTALregulators'><span>Designed TAL regulators</span></a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/TheSwitchMutualRepressorSwitch'><span>Mutual repressor switch</span></a></li> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/TheSwitchMutualRepressorSwitch'><span>Mutual repressor switch</span></a></li> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/TheSwitchPositiveFeedbackLoopSwitch'><span>Positive feedback loop switch</span></a></li> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/TheSwitchPositiveFeedbackLoopSwitch'<ins class="diffchange diffchange-inline">><table class="newtable"><tr class="newtable"><td class="newtable"</ins>><span>Positive feedback loop switch</span<ins class="diffchange diffchange-inline">></td><td class="newtable"><img style="margin-right:-15px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/SafetyMechanismsEscapeTag'><span>Escape tag</span></a></li> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/SafetyMechanismsEscapeTag'><span>Escape tag</span></a></li> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/SafetyMechanismsTermination'><span>Termination</span></a></li> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/SafetyMechanismsTermination'><span>Termination</span></a></li> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><li><a href='https://2012.igem.org/Team:Slovenia/SafetyMechanismsMicrocapsuleDegradation'><span>Microcapsule degradation</span></a></li> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><li><a href='https://2012.igem.org/Team:Slovenia/SafetyMechanismsMicrocapsuleDegradation'<ins class="diffchange diffchange-inline">><table class="newtable"><tr class="newtable"><td class="newtable"</ins>><span>Microcapsule degradation</span<ins class="diffchange diffchange-inline">></td><td class="newtable"><img style="margin-right:-15px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table</ins>></a></li> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td></tr>
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<td colspan="2" class="diff-lineno">Line 342:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ImplementationHepatitisC'><span>Hepatitis C</span></a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ImplementationHepatitisC'><span>Hepatitis C</span></a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ImplementationIschaemicHeartDisease'><span>Ischaemic heart disease</span></a></li> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ImplementationIschaemicHeartDisease'><span>Ischaemic heart disease</span></a></li> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"> <li><a href='https://2012.igem.org/Team:Slovenia/ImplementationImpact'><table class="newtable"><tr class="newtable"><td class="newtable"><span>Impact</span></td><td class="newtable"><img style="margin-right:-86px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table></a></li> </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td></tr>
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<td colspan="2" class="diff-lineno">Line 350:</td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/Modeling'><span>Overview</span></a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/Modeling'><span>Overview</span></a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><li><a href='https://2012.igem.org/Team:Slovenia/ModelingPK'><span>Pharmacokinetics</span></a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><li><a href='https://2012.igem.org/Team:Slovenia/ModelingPK'<ins class="diffchange diffchange-inline">><table class="newtable"><tr class="newtable"><td class="newtable"</ins>><span>Pharmacokinetics</span<ins class="diffchange diffchange-inline">></td><td class="newtable"><img style="margin-right:-15px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table</ins>></a></li> <ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingMethods'><span>Modeling methods</span></a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingMethods'><span>Modeling methods</span></a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingMutualRepressorSwitch'><span>Mutual repressor switch</span></a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingMutualRepressorSwitch'><span>Mutual repressor switch</span></a></li></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingPositiveFeedbackLoopSwitch'><span>Positive feedback loop switch</span></a></li> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingPositiveFeedbackLoopSwitch'><span>Positive feedback loop switch</span></a></li> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingQuantitativeModel'><span><del class="diffchange diffchange-inline">Quantitative and stability </del>model</span></a></li> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/ModelingQuantitativeModel'<ins class="diffchange diffchange-inline">><table class="newtable"><tr class="newtable"><td class="newtable"</ins>><span><ins class="diffchange diffchange-inline">Experimental </ins>model</span<ins class="diffchange diffchange-inline">></td><td class="newtable"><img style="margin-right:-15px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table</ins>></a></li> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><li><a href='https://2012.igem.org/Team:Slovenia/ModelingInteractiveSimulations'><span>Interactive simulations</span></a></li> <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><li><a href='https://2012.igem.org/Team:Slovenia/ModelingInteractiveSimulations'<ins class="diffchange diffchange-inline">><table class="newtable"><tr class="newtable"><td class="newtable"</ins>><span>Interactive simulations</span<ins class="diffchange diffchange-inline">></td><td class="newtable"><img style="margin-right:-15px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table</ins>></a></li> <ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </li></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <ul></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/Notebook'><span>Experimental methods</span></a></li></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/Notebook'><span>Experimental methods</span></a></li></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del><li><a href='https://2012.igem.org/Team:Slovenia/NotebookLablog'><span>Lablog</span></a></li></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins><li><a href='https://2012.igem.org/Team:Slovenia/NotebookLablog'<ins class="diffchange diffchange-inline">><table class="newtable"><tr class="newtable"><td class="newtable"</ins>><span>Lablog</span<ins class="diffchange diffchange-inline">></td><td class="newtable"><img style="margin-right:-90px;" width="25px" src="https://static.igem.org/mediawiki/2012/e/ee/Svn12_hp_new.png"></img></td></tr></table</ins>></a></li> <ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/NotebookLabSafety'><span>Lab safety</span></a></li> </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <li><a href='https://2012.igem.org/Team:Slovenia/NotebookLabSafety'><span>Lab safety</span></a></li> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> </ul></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><a href="https://2012.igem.org/Main_Page"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><div id="dummy" style="background-color:white; position:absolute; left:870px; top:25px; width:115px; height:80px; z-index:100; opacity:0.0;"></ins></div></td></tr>
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</table>Strazkosannhttp://2012.igem.org/wiki/index.php?title=Team:Slovenia/SafetyMechanismsEscapeTag&diff=209055&oldid=prevAljaO at 18:27, 26 September 20122012-09-26T18:27:47Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Though our microencapsulation system is designed in such a way that the capsules are permeable only to nutrients, signalling molecules and produced protein therapeutics, we cannot completely exclude the possibility that some cells could escape from the microcapsules, e.g. due to mechanical damage. We designed <b>a safety mechanism that would ensure that the escaped therapeutic cells could not survive outside microcapsules</b> because of <b>enhanced recognition by the innate immune system</b>.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Though our microencapsulation system is designed in such a way that the capsules are permeable only to nutrients, signalling molecules and produced protein therapeutics, we cannot completely exclude the possibility that some cells could escape from the microcapsules, e.g. due to mechanical damage. We designed <b>a safety mechanism that would ensure that the escaped therapeutic cells could not survive outside microcapsules</b> because of <b>enhanced recognition by the innate immune system</b>.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Innate immune cells, such as natural killer cells (NK cells) and the γδT cells, patrol the tissues of the organism and recognize foreign, infected or damaged cells and destroy them without inflicting dammage to the surrounding tissue. One of the proteins that marks cells for destruction is <b>MICA</b>, a distant homolog of the major histocompatibility complex class I. It has been demonstrated that some cancer cells downregulate MICA to escape recognition (Salih et al., 2002) and that the overexpression of this surface protein marks them for destruction (Groh et al.,1999). MICA is recognized by the activating receptor NKG2D, which is expressed on most natural killer cells, CD8+ T cells, and <del class="diffchange diffchange-inline">??T </del>cells (Bauer et al., 1999), and recognition activates the cytolytic response of NK cells (Steinle et al., 2001) (Figure 1).</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Innate immune cells, such as natural killer cells (NK cells) and the γδT cells, patrol the tissues of the organism and recognize foreign, infected or damaged cells and destroy them without inflicting dammage to the surrounding tissue. One of the proteins that marks cells for destruction is <b>MICA</b>, a distant homolog of the major histocompatibility complex class I. It has been demonstrated that some cancer cells downregulate MICA to escape recognition (Salih et al., 2002) and that the overexpression of this surface protein marks them for destruction (Groh et al.,1999). MICA is recognized by the activating receptor NKG2D, which is expressed on most natural killer cells, CD8+ T cells, and <ins class="diffchange diffchange-inline">γδT </ins>cells (Bauer et al., 1999), and recognition activates the cytolytic response of NK cells (Steinle et al., 2001) (Figure 1).</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our cells should therefore express an abundant amount of MICA protein (an equivalent role could also be fufilled by MICB), which has strong affinity for the NKG2D receptor expressed by NK cells that would recognize and induce apoptosis of the escaped therapeutic cells.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our cells should therefore express an abundant amount of MICA protein (an equivalent role could also be fufilled by MICB), which has strong affinity for the NKG2D receptor expressed by NK cells that would recognize and induce apoptosis of the escaped therapeutic cells.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>AljaOhttp://2012.igem.org/wiki/index.php?title=Team:Slovenia/SafetyMechanismsEscapeTag&diff=209031&oldid=prevAljaO at 18:27, 26 September 20122012-09-26T18:27:14Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Though our microencapsulation system is designed in such a way that the capsules are permeable only to nutrients, signalling molecules and produced protein therapeutics, we cannot completely exclude the possibility that some cells could escape from the microcapsules, e.g. due to mechanical damage. We designed <b>a safety mechanism that would ensure that the escaped therapeutic cells could not survive outside microcapsules</b> because of <b>enhanced recognition by the innate immune system</b>.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Though our microencapsulation system is designed in such a way that the capsules are permeable only to nutrients, signalling molecules and produced protein therapeutics, we cannot completely exclude the possibility that some cells could escape from the microcapsules, e.g. due to mechanical damage. We designed <b>a safety mechanism that would ensure that the escaped therapeutic cells could not survive outside microcapsules</b> because of <b>enhanced recognition by the innate immune system</b>.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Innate immune cells, such as natural killer cells (NK cells) and the <del class="diffchange diffchange-inline">??T </del>cells, patrol the tissues of the organism and recognize foreign, infected or damaged cells and destroy them without inflicting dammage to the surrounding tissue. One of the proteins that marks cells for destruction is <b>MICA</b>, a distant homolog of the major histocompatibility complex class I. It has been demonstrated that some cancer cells downregulate MICA to escape recognition (Salih et al., 2002) and that the overexpression of this surface protein marks them for destruction (Groh et al.,1999). MICA is recognized by the activating receptor NKG2D, which is expressed on most natural killer cells, CD8+ T cells, and ??T cells (Bauer et al., 1999), and recognition activates the cytolytic response of NK cells (Steinle et al., 2001) (Figure 1).</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Innate immune cells, such as natural killer cells (NK cells) and the <ins class="diffchange diffchange-inline">γδT </ins>cells, patrol the tissues of the organism and recognize foreign, infected or damaged cells and destroy them without inflicting dammage to the surrounding tissue. One of the proteins that marks cells for destruction is <b>MICA</b>, a distant homolog of the major histocompatibility complex class I. It has been demonstrated that some cancer cells downregulate MICA to escape recognition (Salih et al., 2002) and that the overexpression of this surface protein marks them for destruction (Groh et al.,1999). MICA is recognized by the activating receptor NKG2D, which is expressed on most natural killer cells, CD8+ T cells, and ??T cells (Bauer et al., 1999), and recognition activates the cytolytic response of NK cells (Steinle et al., 2001) (Figure 1).</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our cells should therefore express an abundant amount of MICA protein (an equivalent role could also be fufilled by MICB), which has strong affinity for the NKG2D receptor expressed by NK cells that would recognize and induce apoptosis of the escaped therapeutic cells.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our cells should therefore express an abundant amount of MICA protein (an equivalent role could also be fufilled by MICB), which has strong affinity for the NKG2D receptor expressed by NK cells that would recognize and induce apoptosis of the escaped therapeutic cells.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>AljaOhttp://2012.igem.org/wiki/index.php?title=Team:Slovenia/SafetyMechanismsEscapeTag&diff=201479&oldid=prevStrazkosann at 15:20, 26 September 20122012-09-26T15:20:09Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!-- VSEBINA OD TU DALJE --></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><!-- VSEBINA OD TU DALJE --></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Escape tag</h1></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h1>Escape tag</h1></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>In the unlikely case that our therapeutic cells escape from the capsules and to prevent their dissemination through the body we designed a safety mechanism that would enhance the recognition and destruction of the escaped therapeutic cells by the innate immune system.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><table class="summary"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><tbody class="summary"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><tr class="summary"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><td class="summary"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>In the unlikely case that our therapeutic cells escape from the capsules and to prevent</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>their dissemination through the body we designed a <ins class="diffchange diffchange-inline"><b></ins>safety mechanism<ins class="diffchange diffchange-inline"></b> </ins>that would <ins class="diffchange diffchange-inline"><b></ins>enhance</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"> </ins>the recognition and destruction<ins class="diffchange diffchange-inline"></b> </ins>of the escaped therapeutic cells by the innate immune system.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We introduced an escape tag that labels the cells with a surface protein that alerts natural killer cells of the host organism to recognize and destroy cells.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We introduced an escape tag that labels the cells with a surface protein that alerts natural killer cells of the host organism to recognize and destroy cells.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>HEK293 cells expressing MICA protein were efficiently killed by human NK cells.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>HEK293 cells expressing MICA protein were efficiently killed by human NK cells.</p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></td></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></tr></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></tbody></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></table></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We considered several variants to ensure destruction of escaped cells such as:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We considered several variants to ensure destruction of escaped cells such as:</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Though our microencapsulation system is designed in such a way that the capsules are permeable only to nutrients, signalling molecules and produced protein therapeutics, we cannot completely exclude the possibility that some cells could escape from the microcapsules, e.g. due to mechanical damage. We designed <b>a safety mechanism that would ensure that the escaped therapeutic cells could not survive outside microcapsules</b> because of <b>enhanced recognition by the innate immune system</b>.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Though our microencapsulation system is designed in such a way that the capsules are permeable only to nutrients, signalling molecules and produced protein therapeutics, we cannot completely exclude the possibility that some cells could escape from the microcapsules, e.g. due to mechanical damage. We designed <b>a safety mechanism that would ensure that the escaped therapeutic cells could not survive outside microcapsules</b> because of <b>enhanced recognition by the innate immune system</b>.</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Innate immune cells, such as natural killer cells (NK cells) and the <del class="diffchange diffchange-inline">γδT </del>cells, patrol the tissues of the organism and recognize foreign, infected or damaged cells and destroy them without inflicting dammage to the surrounding tissue. One of the proteins that marks cells for destruction is <b>MICA</b>, a distant homolog of the major histocompatibility complex class I. It has been demonstrated that some cancer cells downregulate MICA to escape recognition (Salih et al., 2002) and that the overexpression of this surface protein marks them for destruction (Groh et al.,1999). MICA is recognized by the activating receptor NKG2D, which is expressed on most natural killer cells, CD8+ T cells, and <del class="diffchange diffchange-inline">γδT </del>cells (Bauer et al., 1999), and recognition activates the cytolytic response of NK cells (Steinle et al., 2001) (Figure 1).</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Innate immune cells, such as natural killer cells (NK cells) and the <ins class="diffchange diffchange-inline">??T </ins>cells, patrol the tissues of the organism and recognize foreign, infected or damaged cells and destroy them without inflicting dammage to the surrounding tissue. One of the proteins that marks cells for destruction is <b>MICA</b>, a distant homolog of the major histocompatibility complex class I. It has been demonstrated that some cancer cells downregulate MICA to escape recognition (Salih et al., 2002) and that the overexpression of this surface protein marks them for destruction (Groh et al.,1999). MICA is recognized by the activating receptor NKG2D, which is expressed on most natural killer cells, CD8+ T cells, and <ins class="diffchange diffchange-inline">??T </ins>cells (Bauer et al., 1999), and recognition activates the cytolytic response of NK cells (Steinle et al., 2001) (Figure 1).</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our cells should therefore express an abundant amount of MICA protein (an equivalent role could also be fufilled by MICB), which has strong affinity for the NKG2D receptor expressed by NK cells that would recognize and induce apoptosis of the escaped therapeutic cells.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our cells should therefore express an abundant amount of MICA protein (an equivalent role could also be fufilled by MICB), which has strong affinity for the NKG2D receptor expressed by NK cells that would recognize and induce apoptosis of the escaped therapeutic cells.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><table class="invisible" style="width:<del class="diffchange diffchange-inline">90</del>%; text-align:<del class="diffchange diffchange-inline">center</del>;"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><table class="invisible" style="width:<ins class="diffchange diffchange-inline">88</ins>%; text-align:<ins class="diffchange diffchange-inline">justify</ins>;"></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:grey;"></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:grey;"></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Bauer, S., Groh, V., Wu, J., Steinle, A., Phillips, J.H., Lanier, L.L., Spies, T. (1999) Activation of NK Cells and T Cells by NKG2D, a receptor for Stress-Inducible MICA. <i>Science</i> <b>285</b>, 727-729.<br/><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Bauer, S., Groh, V., Wu, J., Steinle, A., Phillips, J.H., Lanier, L.L., Spies, T. (1999) Activation of NK Cells and T Cells by NKG2D, a receptor for Stress-Inducible MICA. <i>Science</i> <b>285</b>, 727-729.<br/><br/></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Borrego, F., Kabat, J., Kim, D.K., Lieto, L., Maasho, K., <del class="diffchange diffchange-inline">Peña</del>, J., Solana, R., Coligan J.E. (2001) Structure and function of major histocompatibility complex (MHC) class I specific receptors expressed on human natural killer (NK) cells. <i>Mol. Immunol.</i> <b>38</b>, 637-660.<br/><br/></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Borrego, F., Kabat, J., Kim, D.K., Lieto, L., Maasho, K., <ins class="diffchange diffchange-inline">Pena</ins>, J., Solana, R., Coligan J.E. (2001) Structure and function of major histocompatibility complex (MHC) class I specific receptors expressed on human natural killer (NK) cells. <i>Mol. Immunol.</i> <b>38</b>, 637-660.<br/><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Groh, V., Rhinehart, R., Secrist, H., Bauer., S., Grabstein, K.H., Spies, T. (1999) Broad tumor-associated expression and recognition by tumor-derived gd T cells of MICA and MICB. <i>Proc. Natl. Acad. Sci.</i> <b>96</b>, 6879–6884.<br/><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Groh, V., Rhinehart, R., Secrist, H., Bauer., S., Grabstein, K.H., Spies, T. (1999) Broad tumor-associated expression and recognition by tumor-derived gd T cells of MICA and MICB. <i>Proc. Natl. Acad. Sci.</i> <b>96</b>, 6879–6884.<br/><br/></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Salih, H.R., Rammensee, H.G., Steinle, A. (2002) Cutting Edge: Down-Regulation of MICA on Human Tumors by Proteolytic Shedding. <i>J Immunol.</i> <b>169</b>, 4098-4102.<br/><br/></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Salih, H.R., Rammensee, H.G., Steinle, A. (2002) Cutting Edge: Down-Regulation of MICA on Human Tumors by Proteolytic Shedding. <i>J Immunol.</i> <b>169</b>, 4098-4102.<br/><br/></div></td></tr>
</table>Strazkosannhttp://2012.igem.org/wiki/index.php?title=Team:Slovenia/SafetyMechanismsEscapeTag&diff=200526&oldid=prevBostjanP at 14:55, 26 September 20122012-09-26T14:55:14Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Though our microencapsulation system is designed in such a way that the capsules are permeable only to nutrients, signalling molecules and produced protein therapeutics, we cannot completely exclude the possibility that some cells could escape from the microcapsules, e.g. due to mechanical damage. We designed <b>a safety mechanism that would ensure that the escaped therapeutic cells could not survive outside microcapsules</b> because of <b>enhanced recognition by the innate immune system</b>.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Though our microencapsulation system is designed in such a way that the capsules are permeable only to nutrients, signalling molecules and produced protein therapeutics, we cannot completely exclude the possibility that some cells could escape from the microcapsules, e.g. due to mechanical damage. We designed <b>a safety mechanism that would ensure that the escaped therapeutic cells could not survive outside microcapsules</b> because of <b>enhanced recognition by the innate immune system</b>.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Innate immune cells, such as natural killer cells (NK cells) and the γδT cells, patrol the tissues of the organism and recognize foreign, infected or damaged cells and destroy them without inflicting dammage to the surrounding tissue. One of the proteins that marks cells for destruction is <b>MICA</b>, a distant homolog of the major histocompatibility complex class I. It has been demonstrated that some cancer cells downregulate MICA to escape recognition (Salih et al., 2002) and that the overexpression of this surface protein marks them for destruction (Groh et al.,1999). MICA is recognized by the activating receptor NKG2D, which is expressed on most natural killer cells, CD8+ T cells, and γδT cells (Bauer et al., 1999), and recognition activates the cytolytic response of NK cells (Steinle et al., 2001) (Figure 1).</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Innate immune cells, such as natural killer cells (NK cells) and the γδT cells, patrol the tissues of the organism and recognize foreign, infected or damaged cells and destroy them without inflicting dammage to the surrounding tissue. One of the proteins that marks cells for destruction is <b>MICA</b>, a distant homolog of the major histocompatibility complex class I. It has been demonstrated that some cancer cells downregulate MICA to escape recognition (Salih et al., 2002) and that the overexpression of this surface protein marks them for destruction (Groh et al.,1999). MICA is recognized by the activating receptor NKG2D, which is expressed on most natural killer cells, CD8+ T cells, and γδT cells (Bauer et al., 1999), and recognition activates the cytolytic response of NK cells (Steinle et al., 2001) (Figure 1).</p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Our cells should therefore express an abundant amount of MICA protein (equivalent role could also be fufilled by MICB), which has strong affinity for the NKG2D receptor expressed by NK cells that would recognize and induce apoptosis of the escaped therapeutic cells.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Our cells should therefore express an abundant amount of MICA protein (<ins class="diffchange diffchange-inline">an </ins>equivalent role could also be fufilled by MICB), which has strong affinity for the NKG2D receptor expressed by NK cells that would recognize and induce apoptosis of the escaped therapeutic cells.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Results</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Results</h2></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Cells <del class="diffchange diffchange-inline">have to </del>express MICA on their surface constitutively since we don't know when a cell might escape. We therefore put MICA under the control of <del class="diffchange diffchange-inline">the </del>constitutive promoter. We transfected HEK293T cells with DNA encoding MICA and BFP and incubated them <del class="diffchange diffchange-inline">with </del>different ratios (1:1, 1:5, 1:10) <del class="diffchange diffchange-inline">of </del>NK-92 cells. Flow cytometry analysis allowed us to quantify the efficiency of recognition and killing of HEK293T expressing MICA escape tag by NK-92 cells.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Cells <ins class="diffchange diffchange-inline">should </ins>express MICA on their surface constitutively since we don't know when a cell might escape. We therefore put MICA under the control of <ins class="diffchange diffchange-inline">a </ins>constitutive promoter. We transfected HEK293T cells with DNA encoding MICA and BFP and incubated them <ins class="diffchange diffchange-inline">in </ins>different ratios (1:1, 1:5, 1:10) <ins class="diffchange diffchange-inline">with </ins>NK-92 cells. Flow cytometry analysis allowed us to quantify the efficiency of recognition and killing of HEK293T expressing MICA escape tag by NK-92 cells.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p>Results demonstrate that HEK293T cells tagged with MICA are destroyed as efficiently as the standard target cells, which are used as a control for the killing assay (Figure 2). This demonstrates that the escape tag is appropriate as an additional safety mechanism to prevent the spreading of the therapeutic cells through the body. The removal of foregin cells would probably be even more efficient <i>in vivo</i>, since the immune system is comprised of several different immune cell types<del class="diffchange diffchange-inline">, </del>apart from NK cells that are capable of recognizing and <del class="diffchange diffchange-inline">destructing </del>foreign cells. However for the real therapeutic application, <i>in vivo</i> tests for the detection of any remaining cells will be neccessary.</p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p>Results demonstrate that HEK293T cells tagged with MICA are destroyed as efficiently as the standard target cells, which are used as a control for the killing assay (Figure 2). This demonstrates that the escape tag is appropriate as an additional safety mechanism to prevent the spreading of the therapeutic cells through the body. The removal of foregin cells would probably be even more efficient <i>in vivo</i>, since the immune system is comprised of several different immune cell types apart from NK cells that are capable of recognizing and <ins class="diffchange diffchange-inline">destroying </ins>foreign cells. However for the real therapeutic application, <i>in vivo</i> tests for the detection of any remaining cells will be neccessary.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2 style="color:grey;">References</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2 style="color:grey;">References</h2></div></td></tr>
</table>BostjanPhttp://2012.igem.org/wiki/index.php?title=Team:Slovenia/SafetyMechanismsEscapeTag&diff=195409&oldid=prevDusanv at 12:36, 26 September 20122012-09-26T12:36:48Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Cells have to express MICA on their surface constitutively since we don't know when a cell might escape. We therefore put MICA under the control of the constitutive promoter. We transfected HEK293T cells with DNA encoding MICA and BFP and incubated them with different ratios (1:1, 1:5, 1:10) of NK-92 cells. Flow cytometry analysis allowed us to quantify the efficiency of recognition and killing of HEK293T expressing MICA escape tag by NK-92 cells.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Cells have to express MICA on their surface constitutively since we don't know when a cell might escape. We therefore put MICA under the control of the constitutive promoter. We transfected HEK293T cells with DNA encoding MICA and BFP and incubated them with different ratios (1:1, 1:5, 1:10) of NK-92 cells. Flow cytometry analysis allowed us to quantify the efficiency of recognition and killing of HEK293T expressing MICA escape tag by NK-92 cells.</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">p</del>><b>Figure 2. The efficiency of NK-92 mediated cell killing.</b> HEK293T cells were transfected with MICA tag. HEK293T cells and NK target cells K562, which were used as a positive control, were stained with CFSE. Target cells (HEK293T and K562) were mixed with NK-92 cells and incubated for 4 h. Cells were then stained with propidium iodide and flow cytometry was used to determine the percentage of specific lysis.</<del class="diffchange diffchange-inline">p</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b>Figure 2. The efficiency of NK-92 mediated cell killing.</b> HEK293T cells were transfected with MICA tag. HEK293T cells and NK target cells K562, which were used as a positive control, were stained with CFSE. Target cells (HEK293T and K562) were mixed with NK-92 cells and incubated for 4 h. Cells were then stained with propidium iodide and flow cytometry was used to determine the percentage of specific lysis.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Results demonstrate that HEK293T cells tagged with MICA are destroyed as efficiently as the standard target cells, which are used as a control for the killing assay (Figure 2). This demonstrates that the escape tag is appropriate as an additional safety mechanism to prevent the spreading of the therapeutic cells through the body. The removal of foregin cells would probably be even more efficient <i>in vivo</i>, since the immune system is comprised of several different immune cell types, apart from NK cells that are capable of recognizing and destructing foreign cells. However for the real therapeutic application, <i>in vivo</i> tests for the detection of any remaining cells will be neccessary.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Results demonstrate that HEK293T cells tagged with MICA are destroyed as efficiently as the standard target cells, which are used as a control for the killing assay (Figure 2). This demonstrates that the escape tag is appropriate as an additional safety mechanism to prevent the spreading of the therapeutic cells through the body. The removal of foregin cells would probably be even more efficient <i>in vivo</i>, since the immune system is comprised of several different immune cell types, apart from NK cells that are capable of recognizing and destructing foreign cells. However for the real therapeutic application, <i>in vivo</i> tests for the detection of any remaining cells will be neccessary.</p></div></td></tr>
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</table>Dusanvhttp://2012.igem.org/wiki/index.php?title=Team:Slovenia/SafetyMechanismsEscapeTag&diff=195300&oldid=prevDusanv at 12:33, 26 September 20122012-09-26T12:33:41Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">Revision as of 12:33, 26 September 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We introduced an escape tag that labels the cells with a surface protein that alerts natural killer cells of the host organism to recognize and destroy cells.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We introduced an escape tag that labels the cells with a surface protein that alerts natural killer cells of the host organism to recognize and destroy cells.</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>HEK293 cells expressing MICA protein were efficiently killed by human NK cells.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>HEK293 cells expressing MICA protein were efficiently killed by human NK cells.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We considered several variants to ensure destruction of escaped cells such as:</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>We considered several variants to ensure destruction of escaped cells such as:</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>After considering many factors we selected the immuno-tagging variant because of its elegant simplicity and since it seems less sensitive to the spontaneous loss of the constructs and leaky apoptosis than other options.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>After considering many factors we selected the immuno-tagging variant because of its elegant simplicity and since it seems less sensitive to the spontaneous loss of the constructs and leaky apoptosis than other options.</p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>The MICA/NKG2D system</h3></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h3>The MICA/NKG2D system</h3></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Innate immune cells, such as natural killer cells (NK cells) and the γδT cells, patrol the tissues of the organism and recognize foreign, infected or damaged cells and destroy them without inflicting dammage to the surrounding tissue. One of the proteins that marks cells for destruction is <b>MICA</b>, a distant homolog of the major histocompatibility complex class I. It has been demonstrated that some cancer cells downregulate MICA to escape recognition (Salih et al., 2002) and that the overexpression of this surface protein marks them for destruction (Groh et al.,1999). MICA is recognized by the activating receptor NKG2D, which is expressed on most natural killer cells, CD8+ T cells, and γδT cells (Bauer et al., 1999), and recognition activates the cytolytic response of NK cells (Steinle et al., 2001) (Figure 1).</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Innate immune cells, such as natural killer cells (NK cells) and the γδT cells, patrol the tissues of the organism and recognize foreign, infected or damaged cells and destroy them without inflicting dammage to the surrounding tissue. One of the proteins that marks cells for destruction is <b>MICA</b>, a distant homolog of the major histocompatibility complex class I. It has been demonstrated that some cancer cells downregulate MICA to escape recognition (Salih et al., 2002) and that the overexpression of this surface protein marks them for destruction (Groh et al.,1999). MICA is recognized by the activating receptor NKG2D, which is expressed on most natural killer cells, CD8+ T cells, and γδT cells (Bauer et al., 1999), and recognition activates the cytolytic response of NK cells (Steinle et al., 2001) (Figure 1).</p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our cells should therefore express an abundant amount of MICA protein (equivalent role could also be fufilled by MICB), which has strong affinity for the NKG2D receptor expressed by NK cells that would recognize and induce apoptosis of the escaped therapeutic cells.</p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p>Our cells should therefore express an abundant amount of MICA protein (equivalent role could also be fufilled by MICB), which has strong affinity for the NKG2D receptor expressed by NK cells that would recognize and induce apoptosis of the escaped therapeutic cells.</p></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">p</del>><b>Figure 1. The principle of our "Escape-tag" safety mechanism.</b></<del class="diffchange diffchange-inline">p</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b>Figure 1. The principle of our "Escape-tag" safety mechanism.</b></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Results</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>Results</h2></div></td></tr>
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</table>Dusanv