Team:Slovenia/Notebook

From 2012.igem.org

(Difference between revisions)
Line 814: Line 814:
<p>In the rapamycin system the gene of interest is under the control of a minimal promoter. The gene's transcription rate is regulated by two proteins that consist of a drug binding domain and either a DNA binding domain or an activation domain. When rapamycin is added both drug binding domains bind to it, consequently joining the activation domain with the DNA binding domain, resulting in a functional transcription factor, which activates the gene of interest. Instead of rapamycin a rapamycin analogue (rapalogue), which is a 1000-fold less imunosupressive than rapamycin, but activates the inducible system like rapamycin, is usualy used as the inducer. (Pollock et al., 2002) </p>
<p>In the rapamycin system the gene of interest is under the control of a minimal promoter. The gene's transcription rate is regulated by two proteins that consist of a drug binding domain and either a DNA binding domain or an activation domain. When rapamycin is added both drug binding domains bind to it, consequently joining the activation domain with the DNA binding domain, resulting in a functional transcription factor, which activates the gene of interest. Instead of rapamycin a rapamycin analogue (rapalogue), which is a 1000-fold less imunosupressive than rapamycin, but activates the inducible system like rapamycin, is usualy used as the inducer. (Pollock et al., 2002) </p>
   
   
-
  <p>TABLE</p>
+
  <!-- table -->
 +
<table class="normal" style="font-size:90%; width:90%; text-align:center;">
 +
<thead class="normal">
 +
<tr class="normal">
 +
 
 +
<th> Regulating vector </th>
 +
 
 +
<th> HetAct </th>
 +
 
 +
</tr>
 +
</thead>
 +
 
 +
 
 +
<tbody  class="normal">
 +
<tr class="normal"><td class="normal"> DNA sequence </td>
 +
                                  <td class="normal"> ZFHD </td>
 +
                  </tr>
 +
 
 +
<tr class="normal"><td class="normal"> Constructs </td>
 +
                                  <td class="normal"> ZFHD_pMIN_TAL-A:KRAB </td>
 +
                  </tr>
 +
 
 +
 
 +
<tr class="normal"><td class="normal"> </td>
 +
                                  <td class="normal"> ZFHD_pMIN_TAL-B:VP16</td>
 +
                  </tr>
 +
 
 +
</tbody>
 +
</table>  

Revision as of 21:17, 26 September 2012