Team:Slovenia/Notebook

From 2012.igem.org

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<p>In the rapamycin system the gene of interest is under the control of a minimal promoter. The gene's transcription rate is regulated by two proteins that consist of a drug binding domain and either a DNA binding domain or an activation domain. When rapamycin is added both drug binding domains bind to it, consequently joining the activation domain with the DNA binding domain, resulting in a functional transcription factor, which activates the gene of interest. Instead of rapamycin a rapamycin analogue (rapalogue), which is a 1000-fold less imunosupressive than rapamycin, but activates the inducible system like rapamycin, is usualy used as the inducer. (Pollock et al., 2002) </p>
<p>In the rapamycin system the gene of interest is under the control of a minimal promoter. The gene's transcription rate is regulated by two proteins that consist of a drug binding domain and either a DNA binding domain or an activation domain. When rapamycin is added both drug binding domains bind to it, consequently joining the activation domain with the DNA binding domain, resulting in a functional transcription factor, which activates the gene of interest. Instead of rapamycin a rapamycin analogue (rapalogue), which is a 1000-fold less imunosupressive than rapamycin, but activates the inducible system like rapamycin, is usualy used as the inducer. (Pollock et al., 2002) </p>
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  <p>TABLE</p>
  <p>TABLE</p>
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The induction systems are described <a href="https://2012.igem.org/Team:Slovenia/Parts">here</a>.
 
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Deuschle, U., Meyer, W.K. and Thiesen, R. (1995) Tetracycline-reversible silencing of eukaryotic promoters. <i>Mol. Cell. Biol. </i> <b>15</b>, 1907–1914.<br/><br/>
Deuschle, U., Meyer, W.K. and Thiesen, R. (1995) Tetracycline-reversible silencing of eukaryotic promoters. <i>Mol. Cell. Biol. </i> <b>15</b>, 1907–1914.<br/><br/>
Kramer, B.P., Fischer, C. and Fussenegger, M. (2004) BioLogic gates enable logical transcription control in mammalian cells. <i> Biotech. Bioeng.</i> <b>87</b>, 478–484.<br/><br/>
Kramer, B.P., Fischer, C. and Fussenegger, M. (2004) BioLogic gates enable logical transcription control in mammalian cells. <i> Biotech. Bioeng.</i> <b>87</b>, 478–484.<br/><br/>
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Pollock, R. and Clackson, T. (2002) Dimerizer-regulated gene expression. <i> Curr. Opin. Biotech. i> <b>13</b>, 459–467.<br/><br/>
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Pollock, R. and Clackson, T. (2002) Dimerizer-regulated gene expression. <i> Curr. Opin. Biotech. </i> <b>13</b>, 459–467.<br/><br/>
Gibson, D.G., Young, L., Chuang, R., Venter J.C., Hutchison III, C. A. and Smith, H.O. (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. <i>Nature methods.</i> <b>6</b>, 343–345.
Gibson, D.G., Young, L., Chuang, R., Venter J.C., Hutchison III, C. A. and Smith, H.O. (2009) Enzymatic assembly of DNA molecules up to several hundred kilobases. <i>Nature methods.</i> <b>6</b>, 343–345.
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Revision as of 20:57, 26 September 2012