Team:Slovenia/Notebook

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<li>Afterwards the glycerol stock was stored at -80 °C. </li>
<li>Afterwards the glycerol stock was stored at -80 °C. </li>
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  <h2><a name="cellcultures"> Cell cultures </a></h2>
  <h2><a name="cellcultures"> Cell cultures </a></h2>
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<h2><a name="inductionsystems"> Induction systems </a></h2>
<h2><a name="inductionsystems"> Induction systems </a></h2>
The induction systems are described <a href="https://2012.igem.org/Team:Slovenia/Parts">here</a>.  
The induction systems are described <a href="https://2012.igem.org/Team:Slovenia/Parts">here</a>.  
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<h2><a name="effectors"> Effectors </a></h2>
<h2><a name="effectors"> Effectors </a></h2>
<h3>Biological assay-anakinra </h3>
<h3>Biological assay-anakinra </h3>
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<h2><a name="microscopy"> Microscopy </a></h2>
<h2><a name="microscopy"> Microscopy </a></h2>
<p>For spatial and temporal imaging of samples a Leica TCS SP5 laser scanning microscope mounted on a Leica DMI 6000 CS inverted microscope (Leica Microsystems, Germany) with a 10× and 20× dry objective and an HCX plan apo 63× oil (NA 1.4) oil immersion objective was used. For image analysis we used ImageJ (Image Processing and Analysis in Java) software (http://rsbweb.nih.gov/ij/) measuring the mean grey values of each cell containing the promoter of interest. </p>
<p>For spatial and temporal imaging of samples a Leica TCS SP5 laser scanning microscope mounted on a Leica DMI 6000 CS inverted microscope (Leica Microsystems, Germany) with a 10× and 20× dry objective and an HCX plan apo 63× oil (NA 1.4) oil immersion objective was used. For image analysis we used ImageJ (Image Processing and Analysis in Java) software (http://rsbweb.nih.gov/ij/) measuring the mean grey values of each cell containing the promoter of interest. </p>
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<h2><a name="flowcytometry"> Flow cytometry </a></h2>
<h2><a name="flowcytometry"> Flow cytometry </a></h2>
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<h2><a name="microencapsulation"> Microencapsulation </a></h2>
<h2><a name="microencapsulation"> Microencapsulation </a></h2>
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<br><b>1,5% alginate</b> (low viscosity) in MOPS buffer (pH = 7,2)</p>
<br><b>1,5% alginate</b> (low viscosity) in MOPS buffer (pH = 7,2)</p>
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<h2><a name="proteindetection"> Protein detection </a></h2>
<h2><a name="proteindetection"> Protein detection </a></h2>

Revision as of 20:18, 26 September 2012