Team:Slovenia/Notebook

From 2012.igem.org

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<p><b>buffers and solutions</b></p>
<p><b>buffers and solutions</b></p>
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<p><b>10 mM MOPS buffer</b> (pH = 7,2) </p>
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<p><b>10 mM MOPS buffer</b> (pH = 7,2)
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<p><b>MOPS buffer with NaCl</b> (pH = 7,2):    10 mM MOPS,    0,85% NaCl</p>
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<p><b>MOPS buffer with NaCl</b> (pH = 7,2):    10 mM MOPS,    0,85% NaCl
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<p><b>Polymerisation solution</b> (pH = 7,2):    10 mM MOPS,    100 mM CaCl2</p>
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<p><b>Polymerisation solution</b> (pH = 7,2):    10 mM MOPS,    100 mM CaCl2
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<p><b>Depolymerisation solution</b> (pH = 7,2):    10 mM MOPS,    50 mM Na3-citrate,    0,45% NaCl</p>
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<p><b>Depolymerisation solution</b> (pH = 7,2):    10 mM MOPS,    50 mM Na3-citrate,    0,45% NaCl
<p><b>0,05% poly-L-lysine</b> (15-30 kDa) in MOPS buffer (pH = 7,3) </p>
<p><b>0,05% poly-L-lysine</b> (15-30 kDa) in MOPS buffer (pH = 7,3) </p>
<p><b>0,03% alginate</b> in MOPS buffer (pH = 7,2) </p>
<p><b>0,03% alginate</b> in MOPS buffer (pH = 7,2) </p>

Revision as of 19:58, 26 September 2012