Team:Slovenia/Notebook

From 2012.igem.org

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<h3>Restriction digest</h3>
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<ol>
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<li>To digest the desired DNA restriction reactions were prepared as follows: </li>
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<span style="margin-left: 30px;><b>for analysis of cloned DNA</b>
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<li>2µl of the appropriate restriction buffer (10X) </li>
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<li>0.5 µL restriction enzyme </li>
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<li>Bring volume to 20 µL with nuclease-free water. </li>
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<li>OR</li>
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<span style="margin-left: 30px;><b>for isolation of specific DNA</b>
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<li>2µl of the appropriate restriction buffer (10X) </li>
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<li>up to 2 µL restriction enzyme </li>
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<li>Bring volume to 50 µL with nuclease-free water. </li></span>
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<li>The sample was incubated at optimal temperature for the restriction enzymes.</li>
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<li>Analysis of fragmented DNA was done by gel electrophoreses. </li>
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<li>Desired DNA fragment was excised and purified using suitable DNA purification kit. </li>
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</ol>
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Revision as of 16:21, 26 September 2012