Revision as of 20:04, 25 September 2012 by Dusanv (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Modeling - mutual repressor switch

  1. Deterministic model
  2. Stochastic model
  3. C#Sim model

Deterministic model of the mutual repressor switch

Deterministic analysis demonstrated that while theoretical conditions under which the mutual repressor switch would exhibit bistability exist, they are unlikely to occur in a realistic experimental setting. This was because:

  • high transcription factor cooperativity of value 2 or above was required for high-level bistability;
  • even for high cooperativity values, the switch was highly intolerant to even very low leaky gene expression, which is always present to a certain extent in an actual cellular environment.

Increase in cooperativity lead to more robust behavior – higher expression levels of stable states were reached and higher – but still low - tolerance to leaky expression was observed.

Experimental results confirmed that the mutual repressor switch did not exhibit bistability.