Team:Slovenia/ModelingInteractiveSimulations

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Next: <a href='https://2012.igem.org/Team:Slovenia/Parts'>Parts >></a>
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Revision as of 19:36, 26 September 2012


Quantitative model interactive simulations


Based on experimental data, we constructed what we refer to as a quantitative model. Please see Quantitative and stability model for details.



Mutual repressor switch

INPUT VECTORS DOSAGE

.
ConstructDosage
[A]_pCMV_TAL-B:KRAB_BFP ng
[B]_pCMV_TAL-A:KRAB_mCitrine ng
pCMV_[PIR]_TAL-B:KRAB ng
pCMV_[ETR]_TAL-A:KRAB ng
pCMV_PIP:KRAB ng
pCMV_E:KRAB ng
(Enter dosages for corresponding plasmids.)


TRANSCRIPTION FACTORS
Equal transcription factors
(Transcription factors will alter transcription with equal rates)


INDUCERS
Pristinamycin
Input times [min]
Durations [min]





Erithromycin
Input times [min]
Durations [min]
(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )

SIMULATION

Time: min
(Simulation running time)


(The results will be shown in a new browser window/tab after few seconds. Have fun.)


Positive feedback loop switch

INPUT VECTORS DOSAGE

.
ConstructDosage
[A]_pCMV_TAL-B:KRAB_TAL-A:VP16_BFP ng
[B]_pCMV_TAL-A:KRAB_TAL-B:VP16_mCitrine ng
pCMV_[PIR]_TAL-B:KRAB_TAL-A:VP16 ng
pCMV_[ETR]_TAL-A:KRAB_TAL-B:VP16 ng
pCMV_PIP:KRAB_E:KRAB ng
(Enter dosages for corresponding plasmids.)


TRANSCRIPTION FACTORS
Equal transcription factors
(Transcription factors will alter transcription with equal rates)


INDUCERS
Pristinamycin
Input times [min]
Durations [min]





Erithromycin
Input times [min]
Durations [min]
(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )


SIMULATION
Time: min
(Simulation running time)


(The results will be shown in a new browser window/tab after few seconds. Have fun.)

Next: Parts >>