Team:Queens Canada/Notebook/Week5


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Notebook - Week 5

Protocol Content

Monday May 28

Throughout the day, we mostly worked again on sponsorship, but we also got some time in the lab and we performed the plasmid dehydration and growing colonies on plates for three different biobrick parts. Essentially the next few days in the lab is going to consist of isolating plasmids from different biobrick parts. The parts we incubated today are BBa_K190019 (fmT), BBa_K346003 (RBS(B0032)+MBP) and BBa_K346001 (MerR). On the wiki side of things, Phillip edited the wiki so that the notebook section is nearly ready to go up!

Tuesday May 29

In the morning, we used GENtle 2 to analyze the D1 and D2 domains for the FliC gene in order to determine the primer sequence for digestion and PCR. We also spent

Wednesday May 30

Thursday May 31

Friday June 1