Team:Queens Canada/Notebook/Week15

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Latest revision as of 22:18, 26 October 2012

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Notebook - Week 15

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Date	Protocol	People	DNA (if relevant)	Quantities and Parameters (if relevant)
08/10/2012	Fast Digestion	Victor	ppFGFP + nFFGFP
08/10/2012	Gel Extraction	Victor	nFFGFP + pFFGFP
08/10/2012	Digestions	Kevin	J48200 w/ pSB1AT3 J13601 Lac operator w/ pSB1A3 746007 Antigen 43 w/ pSB1C3
08/10/2012	digestion	Faisal and David	SmtA and FMT with X and S
08/10/2012	Gel Extraction	Phillip	IPTG (SP), T7 promotor (SP), mCerFC (1,3,4 - 2 trials each)
08/10/2012	Digestion	Phillip	GE IPTG SP [X], GE T7 Prom SP [X]
08/10/2012	Gel Electrophoresis	Phillip		1. ladder 2. GE mCerFC 1,2 3. GE mCerFC 1,1 4. GE mCerFC 3,1 5. GE mCerFC 3,2 6. GE mCerFC 4,1 7. GE mCerFC 4,2 8. DIG GE DIG IPTG SP [X] T1 9. DIG GE DIG IPTG SP [X] T2 10. ladder 11. DIG GE DIG T7 Prom SP [X] T1 12. DIG GE DIG T7 Prom SP [X] T1 13. DIG nFFGFP XP 14. DIG nFFGFP 2 XP 15. DIG I15601 (pSB1A3) XP 16. DIG J45200 Banana Odour XP (pSB1AT3) 17. DIG 346002 Antigen 43 XP (pSB1C3) 18. Ladder
08/10/2012	Fast Digestion	Victor	nFFGFP GE 2, ppFGFP GE 1, MPP Bio-timer k088006, MPP I13601, MPP Bis0A K123000, MPP Antigen 43 K346007	denatured for 14 mins at 37 degrees. Heat inactivated at 80 degrees for 20 mins
08/10/2012	Gel Extraction	Victor and Kevin		1. ladder 2. nFFGFP DIG XP 3. ppFGFP DIG XP 4. DIG Bio-timer k088006 XP 5. MPP Bis0A K123000 XP T2 6. DIG Antigen 43 K346007 XP 7. DIG KI13601 XP
08/10/2012	PCR	Phillip	nFFGFP 1+2, ppFGFP 1+2	25 cycles, 2ul of dna used
08/10/2012	PCR	Beini	mCerFC	25 cycles, 10uL of dna used
08/10/2012	Gel	Kevin		1. ladder 7. J04500 SP dig 8. nFFGFP dig Xp
08/10/2012	Digestion	Phillip	J04500 SP
08/10/2012	Digestion	Beini	GE nFFGFP 1 [XP]
08/10/2012	Gel	Beini	1C3 XP GE, 1A3 BisdA XP GE, I13601 1A2 XP GE, J04500 SP GE, K088006 Timer XP GE pSB1A2, GE pFF GFP XP
08/10/2012	Gel	Beini	mCer FC C, nFF GFP C, T7 prom GE X, IPTG SP GE, DIG GE IPTG SP X, GE T7 prom SP
08/10//2012	Gel extraction	Beini, Kevin	mCer FC, pFF GFP, nFF GFP GE XP, pFF GFP XS	loaded 4 uL loading dye : 16 uL DNA; for digests with FD green, loaded all 30 uL

Given that the vast majority of our work now is in the lab, we will only be updating the "Labwork" section of the Notebook.

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-<p>Notebook - Week 1</p>
+<p>Notebook - Week 15</p>
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-         <div id="protocolcontent">Protocol Content</div>
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+<tr class="tableizer-firstrow"><th>Date</th><th>Protocol</th><th>People</th><th>DNA (if relevant)</th><th>Quantities and Parameters (if relevant)</th></tr> <tr><td>08/10/2012</td><td>Fast Digestion</td><td>Victor</td><td>ppFGFP + nFFGFP</td><td>&nbsp;</td></tr> <tr><td>08/10/2012</td><td>Gel Extraction</td><td>Victor</td><td>nFFGFP + pFFGFP</td><td>&nbsp;</td></tr> <tr><td>08/10/2012</td><td>Digestions </td><td>Kevin</td><td>J48200 w/ pSB1AT3 J13601 Lac operator w/ pSB1A3 746007 Antigen 43 w/ pSB1C3</td><td>&nbsp;</td></tr> <tr><td>08/10/2012</td><td>digestion</td><td>Faisal and David</td><td>SmtA and FMT with X and S</td><td>&nbsp;</td></tr> <tr><td>08/10/2012</td><td>Gel Extraction</td><td>Phillip</td><td>IPTG (SP), T7 promotor (SP), mCerFC (1,3,4 - 2 trials each)</td><td>&nbsp;</td></tr> <tr><td>08/10/2012</td><td>Digestion</td><td>Phillip</td><td>GE IPTG SP [X], GE T7 Prom SP [X]</td><td>&nbsp;</td></tr> <tr><td>08/10/2012</td><td>Gel Electrophoresis </td><td>Phillip</td><td> </td><td>1. ladder 2. GE mCerFC 1,2 3. GE mCerFC 1,1 4. GE mCerFC 3,1 5. GE mCerFC 3,2 6. GE mCerFC 4,1 7. GE mCerFC 4,2 8. DIG GE DIG IPTG SP [X] T1 9. DIG GE DIG IPTG SP [X] T2 10. ladder 11. DIG GE DIG T7 Prom SP [X] T1 12. DIG GE DIG T7 Prom SP [X] T1  13. DIG nFFGFP XP 14. DIG nFFGFP 2 XP 15. DIG I15601 (pSB1A3) XP 16. DIG J45200 Banana Odour XP (pSB1AT3) 17. DIG 346002 Antigen 43 XP (pSB1C3) 18. Ladder</td></tr> <tr><td>08/10/2012</td><td>Fast Digestion</td><td>Victor</td><td>nFFGFP GE 2, ppFGFP GE 1, MPP Bio-timer k088006, MPP I13601, MPP Bis0A K123000, MPP Antigen 43 K346007</td><td>denatured for 14 mins at 37 degrees. Heat inactivated at 80 degrees for 20 mins</td></tr> <tr><td>08/10/2012</td><td>Gel Extraction</td><td>Victor and Kevin</td><td>&nbsp;</td><td>1. ladder 2. nFFGFP DIG XP 3. ppFGFP DIG XP 4. DIG Bio-timer k088006 XP 5. MPP Bis0A K123000 XP T2 6. DIG Antigen 43 K346007 XP 7. DIG KI13601 XP</td></tr> <tr><td>08/10/2012</td><td>PCR</td><td>Phillip</td><td>nFFGFP 1+2, ppFGFP 1+2</td><td>25 cycles, 2ul of dna used</td></tr> <tr><td>08/10/2012</td><td>PCR</td><td>Beini</td><td>mCerFC</td><td>25 cycles, 10uL of dna used</td></tr> <tr><td>08/10/2012</td><td>Gel</td><td>Kevin</td><td>&nbsp;</td><td>1. ladder 7. J04500 SP dig 8. nFFGFP dig Xp </td></tr> <tr><td>08/10/2012</td><td>Digestion</td><td>Phillip</td><td>J04500 SP</td><td>&nbsp;</td></tr> <tr><td>08/10/2012</td><td>Digestion</td><td>Beini</td><td>GE nFFGFP 1 [XP]</td><td>&nbsp;</td></tr> <tr><td>08/10/2012</td><td>Gel</td><td>Beini </td><td>1C3 XP GE, 1A3 BisdA XP GE, I13601 1A2 XP GE, J04500 SP GE, K088006 Timer XP GE pSB1A2, GE pFF GFP XP</td><td>&nbsp;</td></tr> <tr><td>08/10/2012</td><td>Gel  </td><td>Beini</td><td>mCer FC C, nFF GFP C, T7 prom GE X, IPTG SP GE, DIG GE IPTG SP X, GE T7 prom SP</td><td>&nbsp;</td></tr> <tr><td>08/10//2012</td><td>Gel extraction</td><td>Beini, Kevin</td><td>mCer FC, pFF GFP, nFF GFP GE XP, pFF GFP XS</td><td>loaded 4 uL loading dye : 16 uL DNA; for digests with FD green, loaded all 30 uL</td></tr></table>
+</div>
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-<h1> Monday, April 30 </h1>
+<p style="font-size: 2em; text-align:middle'"> Given that the vast majority of our work now is in the lab, we will only be updating the "Labwork" section of the Notebook. </p>
-<h2> <p> The first day! After some introductions and icebreaker activities, Kevin (our Team Manager) gave us a brief introduction of the team structure and showed us how to make our iGEM accounts. After that, we started off our iGEM adventure by researching some past iGEM teams and we each gave a short presentations on the iGEM team that we chose, just to get everyone up to speed with iGEM and get some really cool ideas from past iGEM teams! </p>
-<p> In the afternoon, we continued brainstorming from a list that we had come up with during the school year. We also came up with some new project ideas. To finish off the day, Kevin showed us an old iGEM jamboree presentation. </p> </h2>
-<h1> Tuesday, May 1 </h1>
-<h2> <p> In the morning, we continued brainstorming and went through all our ideas page-by-page. In the afternoon one of our past team members came in to give a presentation about sponsorship, and get us off on the right foot. </p> </h2>
-<h1> Wednesday, May 2 </h1>
-<h2> <p> Today we focused more on brainstorming, and went through our ideas to try and narrow them down to only the most awesome ones. We also started our research into possible sources of sponsorship. </p> </h2>
-<h1> Thursday, May 3 </h1>
-<h2> <p> In the morning we spent some time looking at past posters and wikis by various teams and brainstormed some ideas for our own. Also, one of our advisors, Dr. Chin-Sang, dropped by and we chatted about recent synbio headlines, including making 4-letter codons in E. coli. That night we had our first QGEM social: trivia night at the Grad Club! We didn't win anything, but our team (named C. elegance FTW) was awesome and we had a great time. </p> </h2>
-<h1> Friday, May 4 </h1>
-<h2> <p> Today was spent focused on one thing: narrowing down our list of potential projects. After long hours of debate and many pros and cons charts we finally narrowed it down to 6 possible projects to research in further detail next week. </p> </h2>
+</div>
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