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Team:Penn State/Parts
From 2012.igem.org
(Difference between revisions)
| Line 273: | Line 273: | ||
<td>theonine 9-repeat codon_1</td> | <td>theonine 9-repeat codon_1</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | |||
</tr> | </tr> | ||
| Line 279: | Line 278: | ||
<td>theonine 9-repeat codon_2</td> | <td>theonine 9-repeat codon_2</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
| Line 285: | Line 284: | ||
<td>theonine 9-repeat codon_3</td> | <td>theonine 9-repeat codon_3</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
| Line 291: | Line 290: | ||
<td>theonine 9-repeat codon_alternating</td> | <td>theonine 9-repeat codon_alternating</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
| Line 297: | Line 296: | ||
<td>alanine 9-repeat codon_1</td> | <td>alanine 9-repeat codon_1</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
| Line 303: | Line 302: | ||
<td>alanine 9-repeat codon_2</td> | <td>alanine 9-repeat codon_2</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | |||
</tr> | </tr> | ||
| Line 309: | Line 307: | ||
<td>alanine 9-repeat codon_3</td> | <td>alanine 9-repeat codon_3</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | |||
</tr> | </tr> | ||
| Line 315: | Line 312: | ||
<td>alanine 9-repeat codon_alternating</td> | <td>alanine 9-repeat codon_alternating</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>theonine 6-repeat codon_1</td> | <td>theonine 6-repeat codon_1</td> | ||
| - | <td>complete</td> | + | <td>complete and sequenced</td> |
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>theonine 6-repeat codon_2</td> | <td>theonine 6-repeat codon_2</td> | ||
| - | <td>complete</td> | + | <td>complete and sequenced</td> |
<td>yes</td> | <td>yes</td> | ||
| Line 333: | Line 330: | ||
<td>theonine 6-repeat codon_3</td> | <td>theonine 6-repeat codon_3</td> | ||
<td>complete</td> | <td>complete</td> | ||
| - | + | ||
</tr> | </tr> | ||
| Line 339: | Line 336: | ||
<td>theonine 6-repeat codon_alternating</td> | <td>theonine 6-repeat codon_alternating</td> | ||
<td>complete</td> | <td>complete</td> | ||
| - | + | ||
</tr> | </tr> | ||
| Line 345: | Line 342: | ||
<td>alanine 6-repeat codon_1</td> | <td>alanine 6-repeat codon_1</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
| Line 351: | Line 348: | ||
<td>alanine 6-repeat codon_2</td> | <td>alanine 6-repeat codon_2</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
| Line 357: | Line 354: | ||
<td>alanine 6-repeat codon_3</td> | <td>alanine 6-repeat codon_3</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
| Line 363: | Line 360: | ||
<td>alanine 6-repeat codon_alternating</td> | <td>alanine 6-repeat codon_alternating</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
| Line 369: | Line 366: | ||
<td>threonine 3-repeat codon_1</td> | <td>threonine 3-repeat codon_1</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
| Line 375: | Line 372: | ||
<td>threonine 3-repeat codon_2</td> | <td>threonine 3-repeat codon_2</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
| Line 381: | Line 378: | ||
<td>threonine 3-repeat codon_3</td> | <td>threonine 3-repeat codon_3</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
| Line 446: | Line 443: | ||
<td>RBS1strong_RBS2weak small length</td> | <td>RBS1strong_RBS2weak small length</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1weak_RBS2strong small length</td> | <td>RBS1weak_RBS2strong small length</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1strong_RBS2strong small length</td> | <td>RBS1strong_RBS2strong small length</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1strong_RBS2weak med length</td> | <td>RBS1strong_RBS2weak med length</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1weak_RBS2strong med length</td> | <td>RBS1weak_RBS2strong med length</td> | ||
| - | <td>complete</td> | + | <td>complete and sequenced</td> |
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1strong_RBS2strong med length</td> | <td>RBS1strong_RBS2strong med length</td> | ||
<td>order oligos</td> | <td>order oligos</td> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1strong_RBS2weak med length</td> | <td>RBS1strong_RBS2weak med length</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1weak_RBS2strong med length</td> | <td>RBS1weak_RBS2strong med length</td> | ||
<td>oligos annealed</td> | <td>oligos annealed</td> | ||
| - | + | ||
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>RBS1strong_RBS2strong med length</td> | <td>RBS1strong_RBS2strong med length</td> | ||
<td>order oligos</td> | <td>order oligos</td> | ||
| - | + | ||
</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 02:57, 27 October 2012
Parts Submitted to the Registry
Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!
Submitted Parts
| Name | Type | Description | Symbolic Model | Length (bp) |
|---|---|---|---|---|
| BBa_K933000 | device | multiple start codons coding for RFP and sfGFP respectively | design | |
| BBa_K933001 | device | repeating threonine 6-codon sequence with mCherry and GFP reporters | design | |
| BBa_K933002 | device | AHL leader with mCherry and GFP reporters | design |
Continuing Research
Codon Optimization
| Sequence Name | Status | Construct Sequenced |
|---|---|---|
| theonine 9-repeat codon_1 | oligos annealed | |
| theonine 9-repeat codon_2 | oligos annealed | |
| theonine 9-repeat codon_3 | oligos annealed | |
| theonine 9-repeat codon_alternating | oligos annealed | |
| alanine 9-repeat codon_1 | oligos annealed | |
| alanine 9-repeat codon_2 | oligos annealed | |
| alanine 9-repeat codon_3 | oligos annealed | |
| alanine 9-repeat codon_alternating | oligos annealed | |
| theonine 6-repeat codon_1 | complete and sequenced | |
| theonine 6-repeat codon_2 | complete and sequenced | yes |
| theonine 6-repeat codon_3 | complete | |
| theonine 6-repeat codon_alternating | complete | |
| alanine 6-repeat codon_1 | oligos annealed | |
| alanine 6-repeat codon_2 | oligos annealed | |
| alanine 6-repeat codon_3 | oligos annealed | |
| alanine 6-repeat codon_alternating | oligos annealed | |
| threonine 3-repeat codon_1 | oligos annealed | |
| threonine 3-repeat codon_2 | oligos annealed | |
| threonine 3-repeat codon_3 | oligos annealed | |
| threonine 3-repeat codon_alternating | oligos annealed |
Bidirectional Promoters
| Sequence Name | Status | Part Sequenced |
|---|---|---|
| BBa_K933004 | complete | yes |
| BBa_K933005 | complete and added to Registry | yes |
| BBa_K933006 | complete and added to Registry | yes |
| BBa_K933003 | complete and added to Registry | yes |
| testing promoter BBa_J23103 | oligos annealed | |
| testing promoter BBa_J23114 | oligos annealed |
Multiple Start Codons
| Sequence Name | Status | Part Sequenced |
|---|---|---|
| RBS1strong_RBS2weak small length | oligos annealed | |
| RBS1weak_RBS2strong small length | oligos annealed | |
| RBS1strong_RBS2strong small length | oligos annealed | |
| RBS1strong_RBS2weak med length | oligos annealed | |
| RBS1weak_RBS2strong med length | complete and sequenced | |
| RBS1strong_RBS2strong med length | order oligos | |
| RBS1strong_RBS2weak med length | oligos annealed | |
| RBS1weak_RBS2strong med length | oligos annealed | |
| RBS1strong_RBS2strong med length | order oligos |
