Team:Penn State/Parts

From 2012.igem.org

(Difference between revisions)
(Prototype team page)
 
(31 intermediate revisions not shown)
Line 1: Line 1:
-
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
+
<html xmlns="http://www.w3.org/1999/xhtml" xml:lang="en" lang="en">
 +
<head>
 +
<meta http-equiv="content-type" content="text/html; charset=utf-8" />
 +
<meta name="description" content="your description goes here" />
 +
<meta name="keywords" content="your,keywords,goes,here" />
 +
<meta name="author" content="Your Name" />
 +
<title>Index</title>
 +
<style type="text/css">
 +
body {background:#e0e0e0; color:#303030; font:76% tahoma, verdana, sans-serif; margin:0; padding:15px 0 0;}
 +
#container {
 +
background: #fff;
 +
margin: 0 auto 20px;
 +
padding: 5px 20px 20px;
 +
width: 800px;
 +
}
-
<html>
+
/* Site title */
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
#logo {
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
margin: 15px 0 0;
-
This is a template page. READ THESE INSTRUCTIONS.
+
}
-
</div>
+
#logo h1 a {border:none; color:#505050;}
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
#logo h1 a:hover {border:none; color:#303030; text-decoration:none;}
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
+
#logo p {font-size:1.3em; color:#505050; margin:0 0 20px 0;}
-
</div>
+
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You <strong>MUST</strong>  have all of the pages listed in the menu below with the names specified.  PLEASE keep all of your pages within your teams namespace. 
+
-
</div>
+
-
</div>
+
-
</html>
+
-
<!-- *** End of the alert box *** -->
+
/* Tags */
 +
a {
 +
color: #303030;
 +
text-decoration: none;
 +
border-bottom-width: 1px;
 +
border-top-style: none;
 +
border-right-style: none;
 +
border-bottom-style: none;
 +
border-left-style: none;
 +
border-bottom-color: #505050;
 +
}
 +
a:hover {border-bottom:1px solid #505050; color:#303030; text-decoration:none;}
 +
a img {border:0;}
 +
a:visited {#666;}
 +
h1 {font-size:2.2em; font-weight:400; margin:0 0 5px; padding:0;}
 +
h2 {
 +
font-size: 24pt;
 +
font-weight: bolder;
 +
margin: 0 0 12px;
 +
font-family: "Lucida Sans Unicode", "Lucida Grande", sans-serif;
 +
font-style: normal;
 +
color: #356C80;
 +
text-align: center;
 +
}
 +
h3 {font-size:1.4em; font-weight:400; margin:0 0 10px;}
 +
p {
 +
line-height: 1.5em;
 +
margin: 0 0 15px;
 +
text-align: left;
 +
color: #000;
 +
}
 +
ul,ol {margin:0 0 15px 15px; padding:0;}
 +
li {margin:0 0 3px 0;}
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
 
-
!align="center"|[[Team:Penn_State|Home]]
 
-
!align="center"|[[Team:Penn_State/Team|Team]]
 
-
!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=Penn_State Official Team Profile]
 
-
!align="center"|[[Team:Penn_State/Project|Project]]
 
-
!align="center"|[[Team:Penn_State/Parts|Parts Submitted to the Registry]]
 
-
!align="center"|[[Team:Penn_State/Modeling|Modeling]]
 
-
!align="center"|[[Team:Penn_State/Notebook|Notebook]]
 
-
!align="center"|[[Team:Penn_State/Safety|Safety]]
 
-
!align="center"|[[Team:Penn_State/Attributions|Attributions]]
 
-
|}
 
-
An important aspect of the iGEM competition is the use and creation of standard  biological parts. Each team will make new parts during iGEM and will place them in the [http://partsregistry.org Registry of Standard Biological Parts]. The iGEM software provides an easy way to present the parts your team has created . The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
+
/* Toptabs menu */
 +
#toptabs {font-size:.8em; margin:10px auto -13px; width:760px;}
 +
#toptabs p {color:gray; text-align:right;}
 +
.toptab {background:#f0f0f0 url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #e0e0e0; color:gray; margin:0 0 0 4px; padding:4px 5px 3px; text-decoration:none;}
 +
.toptab:hover {background:#fafafa url(https://2012.igem.org/File:Corner2.gif) top right no-repeat; border-bottom:1px solid #eaeaea; color:#505050; text-decoration:none;}
 +
.activetoptab {
 +
background: #fff url(https://2012.igem.org/File:Corner2.gif) top right no-repeat;
 +
border-bottom: 1px solid #fff;
 +
margin: 0 0 0 4px;
 +
padding: 4px 5px 3px;
 +
text-decoration: none;
 +
color: #282C2D;
 +
}
 +
.activetoptab:hover {border-bottom:1px solid #fff; text-decoration:none;}
-
Remember that the goal of proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for  users who wish to know more.
+
/* Navitabs menu */
 +
#navitabs {clear:both;}
 +
.navitab {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; border-left:1px solid #fff; font-size:1.1em; margin:0 0 4px; padding:4px 8px; text-decoration:none;}
 +
.navitab:hover {background:#d0d0d0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border-bottom:1px solid #fff; text-decoration:none;}
 +
.activenavitab {background:#505050 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; color:#fff; font-size:1.1em; margin:0 0 5px; padding:4px 8px 5px; text-decoration:none;}
 +
.activenavitab:hover {color:#fff; text-decoration:none;}
 +
/* Header description area */
 +
#desc {
 +
clear: both;
 +
color: #fff; /* height:200px; */
 +
margin: 5px 0 15px;
 +
padding: 0 0 5px 0;
 +
background-image: url(https://static.igem.org/mediawiki/2012/a/aa/Nav3.jpg);
 +
background-color: black;
 +
background-repeat: no-repeat;
 +
background-position: left bottom;
 +
text-align: left;
 +
font-size: 18pt;
 +
}
 +
#desc p {
 +
font-size: 10pt;
 +
line-height: 1.3em;
 +
padding: 0 0 0 15px;
 +
width: 325px;
 +
color: #FFF;
 +
}
 +
#desc h3 {
 +
color: #fff;
 +
padding: 15px 15px 0;
 +
text-align: left;
 +
font-size: 18pt;
 +
        text-decoration: none;
 +
        padding-bottom: 10px;
 +
}
 +
#desc a {border-color:#fff; color:#fff; text-decoration:none;}
-
<groupparts>iGEM012 Penn_State</groupparts>
+
/* Main content */
 +
#main {
 +
border-right: 1px solid #d8d8d8;
 +
float: left;
 +
width: 620px;
 +
margin-top: 0;
 +
margin-right: 5;
 +
margin-bottom: 0;
 +
margin-left: 5;
 +
padding-top: 0;
 +
padding-right: 15px;
 +
padding-bottom: 0;
 +
padding-left: 0;
 +
}
 +
 
 +
 
 +
/* Sidebar */
 +
#sidebar {float:right; width:150px;}
 +
#sidebar p {font-size:0.9em; line-height:1.3em; margin:0 0 15px;}
 +
#sidebar ul {margin:0 0 15px 0; padding:0;}
 +
#sidebar li {list-style:none;}
 +
ul.sidelink li {list-style:none; margin:0 0 3px; padding:0;}
 +
ul.sidelink li a{background:#f0f0f0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; display:block; margin:5px 10px 5px 0; padding:3px 4px 3px 8px; text-align:left; width:140px;}
 +
ul.sidelink li a:hover {background:#e0e0e0 url(https://static.igem.org/mediawiki/2012/7/72/Corner.gif) top right no-repeat; border:none; text-decoration:none;}
 +
 
 +
/* Footer */
 +
#footer {background:#fff; border-top:1px solid #d8d8d8; clear:both; margin:0; padding:0;}
 +
#footer a {color:gray;}
 +
#footer a:hover {color:#303030;}
 +
#footer p {color:gray; font-size:1.1em; line-height:1.3em; margin:15px 0 0; padding:0;}
 +
 
 +
/* Additional classes */
 +
.photo {background-color:#f0f0f0; border:1px solid #d8d8d8; margin:0 0 15px; padding:2px;}
 +
.timestamp {font-size:1.2em; color:#606060; margin:-12px 0 12px 0;}
 +
.right {margin:-8px 0 8px 0; text-align:right;}
 +
.block {
 +
padding: 10px;
 +
background-color: #EBEBEB;
 +
font-size: 12px;
 +
}
 +
.block a: {color: #000;
 +
border: none;
 +
text-decoration:none;
 +
}
 +
.block a:hover {
 +
color: #090;
 +
border: none;
 +
cursor: pointer;
 +
text-decoration:none
 +
}
 +
.hide {display:none;
 +
color: #000;
 +
}
 +
 
 +
#blenderAni {
 +
height: 250px;
 +
width: 400px;
 +
background-color: #999;
 +
margin-left: 135px;
 +
}
 +
 
 +
 
 +
tr {
 +
font-family: Tahoma, Geneva, sans-serif;
 +
color: #333;
 +
}
 +
tr a {
 +
font-family: Tahoma, Geneva, sans-serif;
 +
color: #000;
 +
text-decoration: none;
 +
}
 +
tr a:hover {
 +
cursor: pointer;
 +
color: #066;
 +
font-family: Tahoma, Geneva, sans-serif;
 +
text-decoration: none;
 +
}
 +
#logoiGEM {
 +
height: 56px;
 +
width: 140px;
 +
float: right;
 +
background-image: url(https://static.igem.org/mediawiki/2012/e/e9/Sweatshirt_Logofin.jpg);
 +
margin-bottom: 15px;
 +
background-repeat: no-repeat;
 +
}
 +
#psuLogo {
 +
        height: 65px;
 +
        width: 116;
 +
        float: right;
 +
        padding-top: 11px;
 +
}
 +
</style>
 +
</head>
 +
 
 +
 
 +
<body>
 +
<div id="container">
 +
  <div id="logoiGEM"></div>
 +
  <div id="psuLogo"><img src="https://static.igem.org/mediawiki/2012/f/f9/PsuLogo.jpeg"></div>
 +
  <div id="navitabs">
 +
    <h2 class="hide">Sample navigation menu:</h2>
 +
<a class="navitab" href="https://2012.igem.org/Team:Penn_State">Home</a><span class="hide"> | </span>
 +
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Team">Team</a><span class="hide"> | </span>
 +
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Project">Projects</a><span class="hide"> | </span>
 +
<a class="activenavitab" href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a><span class="hide"> | </span>
 +
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Modeling">Main Results</a><span class="hide"> | </span>
 +
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Notebook">Notebook</a><span class="hide"> | </span>
 +
<a class="navitab" href="https://2012.igem.org/Team:Penn_State/Other">Other</a>
 +
</div>
 +
 +
<div id="desc">
 +
<h3>Parts Submitted to the Registry</h3>
 +
<p>Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!</p>
 +
<p class="right"><a href="http://partsregistry.org/Main_Page">Registry of Biological Parts</a></p>
 +
</div>
 +
 
 +
<div id="main">
 +
<h2>Submitted Parts</h2>
 +
<table width="600" border="1">
 +
  <tr>
 +
    <th scope="col">Name</th>
 +
    <th scope="col">Type</th>
 +
    <th scope="col">Description</th>
 +
    <th scope="col">Symbolic Model</th>
 +
    <th scope="col">Length (bp)</th>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933000">BBa_K933000</a></td>
 +
    <td>device</td>
 +
    <td>multiple start codons coding for RFP and sfGFP respectively</td>
 +
    <td><a href="https://static.igem.org/mediawiki/2012/5/54/Msc3.jpg">design</a></td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933001">BBa_K933001</a></td>
 +
    <td>device</td>
 +
    <td>repeating threonine 6-codon sequence with mCherry and GFP reporters</td>
 +
    <td><a href="https://static.igem.org/mediawiki/2012/8/87/Codopt1.jpg">design</a></td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K933002">BBa_K933002</a></td>
 +
    <td>device</td>
 +
    <td>AHL leader with mCherry and GFP reporters</td>
 +
    <td><a href="https://static.igem.org/mediawiki/2012/2/27/Codopt2.jpg">design</a></td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
</table>
 +
</br>
 +
 
 +
<h2>Continuing Research</h2>
 +
<h3><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></h3>
 +
<table width="600" border="1">
 +
  <tr>
 +
    <th scope="col">Sequence Name</th>
 +
    <th scope="col">Status</th>
 +
 
 +
   
 +
  <tr>
 +
    <td>theonine 9-repeat codon_1</td>
 +
    <td>oligos annealed</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>theonine 9-repeat codon_2</td>
 +
    <td>oligos annealed</td>
 +
 
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>theonine 9-repeat codon_3</td>
 +
    <td>oligos annealed</td>
 +
 
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>theonine 9-repeat codon_alternating</td>
 +
    <td>oligos annealed</td>
 +
 
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>alanine 9-repeat codon_1</td>
 +
    <td>oligos annealed</td>
 +
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>alanine 9-repeat codon_2</td>
 +
    <td>oligos annealed</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>alanine 9-repeat codon_3</td>
 +
    <td>oligos annealed</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>alanine 9-repeat codon_alternating</td>
 +
    <td>oligos annealed</td>
 +
 
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>theonine 6-repeat codon_1</td>
 +
    <td>complete and sequenced</td>
 +
 
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>theonine 6-repeat codon_2</td>
 +
    <td>complete and sequenced</td>
 +
    <td>yes</td>
 +
  </tr>
 +
  <tr>
 +
    <td>theonine 6-repeat codon_3</td>
 +
    <td>complete</td>
 +
 
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>theonine 6-repeat codon_alternating</td>
 +
    <td>complete</td>
 +
 
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>alanine 6-repeat codon_1</td>
 +
    <td>oligos annealed</td>
 +
 
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>alanine 6-repeat codon_2</td>
 +
    <td>oligos annealed</td>
 +
 
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>alanine 6-repeat codon_3</td>
 +
    <td>oligos annealed</td>
 +
 
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>alanine 6-repeat codon_alternating</td>
 +
    <td>oligos annealed</td>
 +
 
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>threonine 3-repeat codon_1</td>
 +
    <td>oligos annealed</td>
 +
 
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>threonine 3-repeat codon_2</td>
 +
    <td>oligos annealed</td>
 +
 
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>threonine 3-repeat codon_3</td>
 +
    <td>oligos annealed</td>
 +
 
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>threonine 3-repeat codon_alternating</td>
 +
    <td>oligos annealed</td>
 +
    <td>&nbsp;</td>
 +
 
 +
  </tr>
 +
 
 +
</table>
 +
<br/>
 +
<h3><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters
 +
">Bidirectional Promoters</a></h3>
 +
 
 +
<table width="600" border="1">
 +
  <tr>
 +
    <th scope="col">Sequence Name</th>
 +
    <th scope="col">Status</th>
 +
    <th scope="col">Part Sequenced</th>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://partsregistry.org/Part:BBa_K933004">BBa_K933004</a></td>
 +
    <td>complete</td>
 +
    <td>yes</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://partsregistry.org/Part:BBa_K933005">BBa_K933005</a></td>
 +
    <td>complete and added to Registry</td>
 +
    <td>yes</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://partsregistry.org/Part:BBa_K933006">BBa_K933006</a></td>
 +
    <td>complete and added to Registry</td>
 +
    <td>yes</td>
 +
  </tr>
 +
  <tr>
 +
    <td><a href="http://partsregistry.org/Part:BBa_K933003">BBa_K933003</a></td>
 +
    <td>complete and added to Registry</td>
 +
    <td>yes</td>
 +
  </tr>
 +
  <tr>
 +
    <td>testing promoter BBa_J23103</td>
 +
    <td>oligos annealed</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
  <tr>
 +
    <td>testing promoter BBa_J23114</td>
 +
    <td>oligos annealed</td>
 +
    <td>&nbsp;</td>
 +
  </tr>
 +
</table>
 +
 
 +
<br/>
 +
<h3><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></h3>
 +
 
 +
<table width="600" border="1">
 +
  <tr>
 +
    <th scope="col">Sequence Name</th>
 +
    <th scope="col">Status</th>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1strong_RBS2weak small length</td>
 +
    <td>oligos annealed</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1weak_RBS2strong small length</td>
 +
    <td>oligos annealed</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1strong_RBS2strong small length</td>
 +
    <td>oligos annealed</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1strong_RBS2weak med length</td>
 +
    <td>oligos annealed</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1weak_RBS2strong med length</td>
 +
    <td>complete and sequenced</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1strong_RBS2strong med length</td>
 +
    <td>order oligos</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1strong_RBS2weak med length</td>
 +
    <td>oligos annealed</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1weak_RBS2strong med length</td>
 +
    <td>oligos annealed</td>
 +
 
 +
  </tr>
 +
  <tr>
 +
    <td>RBS1strong_RBS2strong med length</td>
 +
    <td>order oligos</td>
 +
 
 +
  </tr>
 +
</table>
 +
</div>
 +
 
 +
<div id="sidebar">
 +
<h3>Check these Out!</h3>
 +
<ul class="sidelink">
 +
<li><a href="https://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li>
 +
<li><a href="https://2012.igem.org/Team:Penn_State/Parts">Parts</a></li>
 +
<li><a href="https://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li>
 +
</ul>
 +
 
 +
 +
<h3>Projects</h3>
 +
    <ul>
 +
      <li><a href="https://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li>
 +
      <li><a href="https://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">Bidirectional Promoters</a></li>
 +
      <li><a href="https://2012.igem.org/Team:Penn_State/Codon_Optimization">Codon Optimization</a></li>
 +
    </ul>
 +
    </div>
 +
<div id="footer">
 +
<p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p>
 +
</div>
 +
 
 +
</div>
 +
</body>
 +
</html>

Latest revision as of 03:01, 27 October 2012

Index

Parts Submitted to the Registry

Check out the parts we have submitted to the Registry of Standard Biological Parts! This year's parts include two of our main project constructs, as well as one successful test construct. Be on the lookout for many more to come!

Registry of Biological Parts

Submitted Parts

Name Type Description Symbolic Model Length (bp)
BBa_K933000 device multiple start codons coding for RFP and sfGFP respectively design  
BBa_K933001 device repeating threonine 6-codon sequence with mCherry and GFP reporters design  
BBa_K933002 device AHL leader with mCherry and GFP reporters design  

Continuing Research

Codon Optimization

Sequence Name Status
theonine 9-repeat codon_1 oligos annealed
theonine 9-repeat codon_2 oligos annealed
theonine 9-repeat codon_3 oligos annealed
theonine 9-repeat codon_alternating oligos annealed
alanine 9-repeat codon_1 oligos annealed
alanine 9-repeat codon_2 oligos annealed
alanine 9-repeat codon_3 oligos annealed
alanine 9-repeat codon_alternating oligos annealed
theonine 6-repeat codon_1 complete and sequenced
theonine 6-repeat codon_2 complete and sequenced yes
theonine 6-repeat codon_3 complete
theonine 6-repeat codon_alternating complete
alanine 6-repeat codon_1 oligos annealed
alanine 6-repeat codon_2 oligos annealed
alanine 6-repeat codon_3 oligos annealed
alanine 6-repeat codon_alternating oligos annealed
threonine 3-repeat codon_1 oligos annealed
threonine 3-repeat codon_2 oligos annealed
threonine 3-repeat codon_3 oligos annealed
threonine 3-repeat codon_alternating oligos annealed  

Bidirectional Promoters

Sequence Name Status Part Sequenced
BBa_K933004 complete yes
BBa_K933005 complete and added to Registry yes
BBa_K933006 complete and added to Registry yes
BBa_K933003 complete and added to Registry yes
testing promoter BBa_J23103 oligos annealed  
testing promoter BBa_J23114 oligos annealed  

Multiple Start Codons

Sequence Name Status
RBS1strong_RBS2weak small length oligos annealed
RBS1weak_RBS2strong small length oligos annealed
RBS1strong_RBS2strong small length oligos annealed
RBS1strong_RBS2weak med length oligos annealed
RBS1weak_RBS2strong med length complete and sequenced
RBS1strong_RBS2strong med length order oligos
RBS1strong_RBS2weak med length oligos annealed
RBS1weak_RBS2strong med length oligos annealed
RBS1strong_RBS2strong med length order oligos

Retrieved from "http://2012.igem.org/Team:Penn_State/Parts"