http://2012.igem.org/wiki/index.php?title=Team:Penn/Parts&feed=atom&action=historyTeam:Penn/Parts - Revision history2024-03-28T23:11:42ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Penn/Parts&diff=298540&oldid=prevAvinv at 03:59, 27 October 20122012-10-27T03:59:37Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name" align="center">Parts Submitted</div></b><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name" align="center">Parts Submitted</div></b><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><b><div class="name">INPNC-MCS (Multiple Cloning Site)</div></b></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><b><div class="name">INPNC-MCS (Multiple Cloning Site)<ins class="diffchange diffchange-inline">: Winner of "Best BioBrick, Engineered" at Americas East</ins></div></b></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A multiple cloning site version of Ice Nucleation Protein that can be used for surface display experiments. The associated MCS and GS short amino acid linker allows for rapid generation of fusion proteins for surface display. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811005"> BBa_K811005 </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A multiple cloning site version of Ice Nucleation Protein that can be used for surface display experiments. The associated MCS and GS short amino acid linker allows for rapid generation of fusion proteins for surface display. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811005"> BBa_K811005 </a></p></div></td></tr>
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</table>Avinvhttp://2012.igem.org/wiki/index.php?title=Team:Penn/Parts&diff=296893&oldid=prevQiaop at 03:06, 27 October 20122012-10-27T03:06:36Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name" align="center">Parts Submitted</div></b><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name" align="center">Parts Submitted</div></b><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><b><div class="name">INPNC-MCS (Multiple Cloning Site)</div></b></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">A multiple cloning site version of Ice Nucleation Protein that can be used for surface display experiments. The associated MCS and GS short amino acid linker allows for rapid generation of fusion proteins for surface display. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811005"> BBa_K811005 </a></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">ClyA</div></b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">ClyA</div></b></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See <a href="http://partsregistry.org/Part:BBa_K811000">BBa_K811000</a> </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See <a href="http://partsregistry.org/Part:BBa_K811000">BBa_K811000</a> </p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811004"> BBa_K811004 </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811004"> BBa_K811004 </a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><b><div class="name">INPNC-MCS (Multiple Cloning Site)</div></b></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">A multiple cloning site version of Ice Nucleation Protein that can be used for surface display experiments. The associated MCS and GS short amino acid linker allows for rapid generation of fusion proteins for surface display. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811005"> BBa_K811005 </a></p></del></div></td><td colspan="2"> </td></tr>
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</table>Qiaophttp://2012.igem.org/wiki/index.php?title=Team:Penn/Parts&diff=283054&oldid=prevAmurthur at 10:31, 26 October 20122012-10-26T10:31:20Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">INPNC-HA</div></b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">INPNC-HA</div></b></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part<del class="diffchange diffchange-inline">=</del>BBa_K811004"> BBa_K811004 </a></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part<ins class="diffchange diffchange-inline">:</ins>BBa_K811004"> BBa_K811004 </a></p></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">INPNC-MCS (Multiple Cloning Site)</div></b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">INPNC-MCS (Multiple Cloning Site)</div></b></div></td></tr>
</table>Amurthurhttp://2012.igem.org/wiki/index.php?title=Team:Penn/Parts&diff=274891&oldid=prevAmurthur at 17:52, 23 October 20122012-10-23T17:52:55Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">ClyA</div></b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">ClyA</div></b></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See <a href="http://partsregistry.org/Part:BBa_K811000">BBa_K811000</a> </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See <a href="http://partsregistry.org/Part:BBa_K811000">BBa_K811000</a> </p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><b><div class="name">His-ClyA</div></b></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><b><div class="name">His-ClyA</div></b></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A N-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811001"> BBa_K811001 </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A N-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811001"> BBa_K811001 </a></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><b><div class="name">ClyA-His</div></b></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><b><div class="name">ClyA-His</div></b></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811002"> BBa_K811002 </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811002"> BBa_K811002 </a></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"><b><div class="name">INPNC</div></b></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><b><div class="name">INPNC</div></b></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A truncated N-C terminus version of Ice Nucleation Protein that can be used for surface display experiments. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811003"> BBa_K811003 </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A truncated N-C terminus version of Ice Nucleation Protein that can be used for surface display experiments. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811003"> BBa_K811003 </a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><br></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">INPNC-HA</div></b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">INPNC-HA</div></b></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part=BBa_K811004"> BBa_K811004 </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part=BBa_K811004"> BBa_K811004 </a></p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">INPNC-MCS (Multiple Cloning Site)</div></b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">INPNC-MCS (Multiple Cloning Site)</div></b></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A multiple cloning site version of Ice Nucleation Protein that can be used for surface display experiments. The associated MCS and GS short amino acid linker allows for rapid generation of fusion proteins for surface display. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811005"> BBa_K811005 </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A multiple cloning site version of Ice Nucleation Protein that can be used for surface display experiments. The associated MCS and GS short amino acid linker allows for rapid generation of fusion proteins for surface display. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811005"> BBa_K811005 </a></p></div></td></tr>
</table>Amurthurhttp://2012.igem.org/wiki/index.php?title=Team:Penn/Parts&diff=274889&oldid=prevAmurthur at 17:52, 23 October 20122012-10-23T17:52:19Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See <a href="http://partsregistry.org/Part:BBa_K811000">BBa_K811000</a> </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See <a href="http://partsregistry.org/Part:BBa_K811000">BBa_K811000</a> </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">His-ClyA</div></b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">His-ClyA</div></b></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A N-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811001"> BBa_K811001 </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A N-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811001"> BBa_K811001 </a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">ClyA-His</div></b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">ClyA-His</div></b></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811002"> BBa_K811002 </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811002"> BBa_K811002 </a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">INPNC</div></b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">INPNC</div></b></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A truncated N-C terminus version of Ice Nucleation Protein that can be used for surface display experiments. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811003"> BBa_K811003 </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A truncated N-C terminus version of Ice Nucleation Protein that can be used for surface display experiments. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811003"> BBa_K811003 </a></p></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">INPNC-HA</div></b></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><b><div class="name">INPNC-HA</div></b></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part=BBa_K811004"> BBa_K811004 </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part=BBa_K811004"> BBa_K811004 </a></p></div></td></tr>
</table>Amurthurhttp://2012.igem.org/wiki/index.php?title=Team:Penn/Parts&diff=274878&oldid=prevAmurthur at 17:46, 23 October 20122012-10-23T17:46:03Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></style></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><br /></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">!-----------------------------------------------------------------------------------------------------</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">div class="bigbox"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><b><div class="name" align="center">Parts Submitted</div></b><br</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">div style="background-color:white;padding:20px 60px 20px 60px; text-align:justify;"</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">b</ins>><<ins class="diffchange diffchange-inline">div class</ins>="<ins class="diffchange diffchange-inline">name</ins>"><ins class="diffchange diffchange-inline">ClyA</ins></<ins class="diffchange diffchange-inline">div</ins>></<ins class="diffchange diffchange-inline">b</ins>></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">h1 align</del>="<del class="diffchange diffchange-inline">center</del>"><del class="diffchange diffchange-inline">Parts Submitted</del></<del class="diffchange diffchange-inline">h1</del>><del class="diffchange diffchange-inline"><br></del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><h2>ClyA</del></<del class="diffchange diffchange-inline">h2</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See <a href="http://partsregistry.org/Part:BBa_K811000">BBa_K811000</a> </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See <a href="http://partsregistry.org/Part:BBa_K811000">BBa_K811000</a> </p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">h2</del>>His-ClyA</<del class="diffchange diffchange-inline">h2</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">b><div class="name"</ins>>His-ClyA</<ins class="diffchange diffchange-inline">div></b</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A N-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811001"> BBa_K811001 </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A N-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811001"> BBa_K811001 </a></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">h2</del>>ClyA-His</<del class="diffchange diffchange-inline">h2</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">b><div class="name"</ins>>ClyA-His</<ins class="diffchange diffchange-inline">div></b</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811002"> BBa_K811002 </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/Part:BBa_K811002"> BBa_K811002 </a></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">h2</del>>INPNC</<del class="diffchange diffchange-inline">h2</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">b><div class="name"</ins>>INPNC</<ins class="diffchange diffchange-inline">div></b</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A truncated N-C terminus version of Ice Nucleation Protein that can be used for surface display experiments. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811003"> BBa_K811003 </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A truncated N-C terminus version of Ice Nucleation Protein that can be used for surface display experiments. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811003"> BBa_K811003 </a></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">h2</del>>INPNC-HA</<del class="diffchange diffchange-inline">h2</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">b><div class="name"</ins>>INPNC-HA</<ins class="diffchange diffchange-inline">div></b</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part=BBa_K811004"> BBa_K811004 </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part=BBa_K811004"> BBa_K811004 </a></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">h2</del>>INPNC-MCS (Multiple Cloning Site)</<del class="diffchange diffchange-inline">h2</del>></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><<ins class="diffchange diffchange-inline">b><div class="name"</ins>>INPNC-MCS (Multiple Cloning Site)</<ins class="diffchange diffchange-inline">div></b</ins>></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A multiple cloning site version of Ice Nucleation Protein that can be used for surface display experiments. The associated MCS and GS short amino acid linker allows for rapid generation of fusion proteins for surface display. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811005"> BBa_K811005 </a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>A multiple cloning site version of Ice Nucleation Protein that can be used for surface display experiments. The associated MCS and GS short amino acid linker allows for rapid generation of fusion proteins for surface display. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/Part:BBa_K811005"> BBa_K811005 </a></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;"></del></div></td><td colspan="2"> </td></tr>
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</table>Amurthurhttp://2012.igem.org/wiki/index.php?title=Team:Penn/Parts&diff=273788&oldid=prevAmurthur at 13:55, 22 October 20122012-10-22T13:55:14Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><h1>Parts Submitted</h1></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><h1 <ins class="diffchange diffchange-inline">align="center"</ins>>Parts Submitted</h1<ins class="diffchange diffchange-inline">><br</ins>></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>ClyA</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>ClyA</h2></div></td></tr>
</table>Amurthurhttp://2012.igem.org/wiki/index.php?title=Team:Penn/Parts&diff=273787&oldid=prevAmurthur at 13:54, 22 October 20122012-10-22T13:54:35Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><div style="background-color:white;padding<del class="diffchange diffchange-inline">-left</del>:20px; <del class="diffchange diffchange-inline">padding</del>-<del class="diffchange diffchange-inline">right</del>:<del class="diffchange diffchange-inline">20px</del>;"></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><div style="background-color:white;padding:20px <ins class="diffchange diffchange-inline">60px 20px 60px</ins>; <ins class="diffchange diffchange-inline">text</ins>-<ins class="diffchange diffchange-inline">align</ins>:<ins class="diffchange diffchange-inline">justify</ins>;"></div></td></tr>
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</table>Amurthurhttp://2012.igem.org/wiki/index.php?title=Team:Penn/Parts&diff=269637&oldid=prevAmurthur at 08:44, 15 October 20122012-10-15T08:44:59Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>ClyA</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>ClyA</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="color:black"> Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See <a href="http://partsregistry.org/<del class="diffchange diffchange-inline">partsdb/edit_seq.cgi?part=</del>BBa_K811000">BBa_K811000</a> </p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="color:black"> Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See <a href="http://partsregistry.org/<ins class="diffchange diffchange-inline">Part:</ins>BBa_K811000">BBa_K811000</a> </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>His-ClyA</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>His-ClyA</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="color:black"> A N-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/<del class="diffchange diffchange-inline">partsdb/edit_seq.cgi?part=</del>BBa_K811001"> BBa_K811001 </a></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="color:black"> A N-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/<ins class="diffchange diffchange-inline">Part:</ins>BBa_K811001"> BBa_K811001 </a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>ClyA-His</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>ClyA-His</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/<del class="diffchange diffchange-inline">partsdb/edit_seq.cgi?part=</del>BBa_K811002"> BBa_K811002 </a></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus his-tagged version of clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/<ins class="diffchange diffchange-inline">Part:</ins>BBa_K811002"> BBa_K811002 </a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>INPNC</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>INPNC</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="color:black"> A truncated N-C terminus version of Ice Nucleation Protein that can be used for surface display experiments. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/<del class="diffchange diffchange-inline">partsdb/edit_seq.cgi?part=</del>BBa_K811003"> BBa_K811003 </a></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="color:black"> A truncated N-C terminus version of Ice Nucleation Protein that can be used for surface display experiments. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/<ins class="diffchange diffchange-inline">Part:</ins>BBa_K811003"> BBa_K811003 </a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>INPNC-HA</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>INPNC-HA</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/<del class="diffchange diffchange-inline">partsdb/edit_seq.cgi?part</del>=BBa_K811004"> BBa_K811004 </a></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/<ins class="diffchange diffchange-inline">Part</ins>=BBa_K811004"> BBa_K811004 </a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>INPNC-MCS (Multiple Cloning Site)</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>INPNC-MCS (Multiple Cloning Site)</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>A multiple cloning site version of Ice Nucleation Protein that can be used for surface display experiments. The associated MCS and GS short amino acid linker allows for rapid generation of fusion proteins for surface display. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/<del class="diffchange diffchange-inline">partsdb/edit_seq.cgi?part=BBa_K811004</del>"> <del class="diffchange diffchange-inline">BBa_K811004 </del></a></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>A multiple cloning site version of Ice Nucleation Protein that can be used for surface display experiments. The associated MCS and GS short amino acid linker allows for rapid generation of fusion proteins for surface display. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/<ins class="diffchange diffchange-inline">Part:BBa_K811005</ins>"> <ins class="diffchange diffchange-inline">BBa_K811005 </ins></a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
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</table>Amurthurhttp://2012.igem.org/wiki/index.php?title=Team:Penn/Parts&diff=265918&oldid=prevQiaop at 02:55, 4 October 20122012-10-04T02:55:11Z<p></p>
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<td colspan='2' style="background-color: white; color:black;">← Older revision</td>
<td colspan='2' style="background-color: white; color:black;">Revision as of 02:55, 4 October 2012</td>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See <a href="http://partsregistry.org/partsdb/edit_seq.cgi?part=BBa_K811000">BBa_K811000</a> </p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="color:black"> Cytolysin A is a 34kDa protein that is capable of inducing rapid cell lysis in mammalian cells through the formation of a pore complex in the plasma membrane. See <a href="http://partsregistry.org/partsdb/edit_seq.cgi?part=BBa_K811000">BBa_K811000</a> </p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>His-ClyA</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>His-ClyA</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="color:black"> A N-terminus his-tagged version clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/partsdb/edit_seq.cgi?part=BBa_K811001"> BBa_K811001 </a></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="color:black"> A N-terminus his-tagged version <ins class="diffchange diffchange-inline">of </ins>clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/partsdb/edit_seq.cgi?part=BBa_K811001"> BBa_K811001 </a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>ClyA-His</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>ClyA-His</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus his-tagged version clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/partsdb/edit_seq.cgi?part=BBa_K811002"> BBa_K811002 </a></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="color:black"> A C-terminus his-tagged version <ins class="diffchange diffchange-inline">of </ins>clyA that is ideal for IMAC protein purification and western blotting. See <a href="http://partsregistry.org/partsdb/edit_seq.cgi?part=BBa_K811002"> BBa_K811002 </a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>INPNC</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>INPNC</h2></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p style="color:black"> A truncated N-C terminus version of Ice Nucleation Protein that can be used for surface display experiments. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/partsdb/edit_seq.cgi?part=BBa_K811003"> BBa_K811003 </a></p></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>INPNC-HA</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>INPNC-HA</h2></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><p style="color:black"> A C-terminus HA-tagged version of Ice Nucleation Protein that can be used for surface display experiments. The HA tag allows for visualization of INPNC localization through immunocytochemistry and western blotting. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/partsdb/edit_seq.cgi?part=BBa_K811004"> BBa_K811004 </a></p></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>INPNC-MCS (Multiple Cloning Site)</h2></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><h2>INPNC-MCS (Multiple Cloning Site)</h2></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">A multiple cloning site version of Ice Nucleation Protein that can be used for surface display experiments. The associated MCS and GS short amino acid linker allows for rapid generation of fusion proteins for surface display. Expression of INPNC fusion proteins will traffic to the cell membrane. See <a href="http://partsregistry.org/partsdb/edit_seq.cgi?part=BBa_K811004"> BBa_K811004 </a></p></ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Qiaop