Team:Peking/Project/Phototaxis

Introduction

Bacterial phototaxis refers to the ability of bacteria to sense light changes in their extracellular environment and to bias their motility towards or away from the light. Phototactic responses are observed in many organisms such as Serratia marcescens, Tetrahymena, and Euglena. The behavior of phototaxis is the directed movement up a gradient to an increasing amount of light. This is analogous to positive chemotaxis： the usage of light as the attractant rather than chemicals.

Figure 2. The simplified scheme of protein-protein interactions during chemotaxis of bacteria.

Among those proteins, CheY have the direct attachment to the flagella motors. Phosphorylated CheY diffuses through the cytoplasm to the motors，binding to FliM, a component of the C ring of the flagellar motor, and induces CW rotation of the motor. CheZ can dephosphorylate CheY, resulting in CCW rotations which leads to the smooth swimming. CheZ mutants result into swarming while CheY mutants are smooth-swimming.

CheZ is commonly used to affect bacterial motion in synthetic biology. Experiments shows that different levels of CheZ induced by arabinose can change the diameter of the swarming colony, combination between the quorum sensing part and CheZ can make bacteria form a stripe pattern. So we suppose combination between CheZ and our Luminesensor must have an effect on bacteria in motion. As modeling reveals, it does have a phototaxis phenomenon where light is a negative stimuli, showing that it is very promising to build a ultra-sensitive, effective, promising phototaxis design.