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Team:Peking/Modeling/Ring
From 2012.igem.org
Background
Genetic engineering circuits in E.coli enable cells to perform programmably; however, more complex functions are limited by leakage of the gene expression. Consider that cells are able to detect environmental signals such as explosives (e.g., RDX and TNT), toxins, metals, salinity, pH, temperature and light, cell-cell communication-based multicellular networks provide an extended vista for synthetic biology.
As a hallmark of coordinated cellular behavior, pattern formation typically required cell-cell communication and intracellular signal processing. For more site-specific signaling and pattern formation, light may be more appropriate alternative. Due to the high sensitivity of our Luminesensor, it is possible to construct a ring-like pattern based on light-communication, previously done by AHL.
Circuit Design
Figure 1 illustrates the design of the synthetic multicellular system. There are two kinds of E. coli cells on the plate [1]:
Figure 1. Genetic Circuit of Ring Pattern Formation.
Reference
- 1. Subhayu Basu et al.(2005), A synthetic multicellular system for programmed pattern formation. Nature, vol.434: 1130: 1134
